Restriction map for
pFN21AE2013
(Linear) (Six-base) MAPSORT of: KIEE2013.seq Check: 3588 from: 1 to: 1773
KIEE2013.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 580 1773
Size: 580 1193
AccI GT'mk_AC
Cuts at: 0 1175 1773
Size: 1175 598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1736 1773
Size: 937 799 37
Fragments arranged by size:
937 799 37
AflIII A'CryG_T
Cuts at: 0 223 1773
Size: 223 1550
AleI CACnn'nnGTG
Cuts at: 0 1255 1773
Size: 1255 518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 172 612 1773
Size: 172 440 1161
Fragments arranged by size:
1161 440 172
AlwNI CAG_nnn'CTG
Cuts at: 0 1042 1326 1476 1747 1773
Size: 1042 284 150 271 26
Fragments arranged by size:
1042 284 271 150 26
ApaI G_GGCC'C
Cuts at: 0 565 1773
Size: 565 1208
ApaLI G'TGCA_C
Cuts at: 0 380 1773
Size: 380 1393
ApoI r'AATT_y
Cuts at: 0 6 537 682 1773
Size: 6 531 145 1091
Fragments arranged by size:
1091 531 145 6
BamHI G'GATC_C
Cuts at: 0 1582 1773
Size: 1582 191
BanI G'GyrC_C
Cuts at: 0 30 1159 1773
Size: 30 1129 614
Fragments arranged by size:
1129 614 30
BanII G_rGCy'C
Cuts at: 0 565 1773
Size: 565 1208
BbeI G_GCGC'C
Cuts at: 0 34 1773
Size: 34 1739
BbsI GAAGACnn'nnnn_
Cuts at: 0 329 1655 1683 1773
Size: 329 1326 28 90
Fragments arranged by size:
1326 329 90 28
BclI T'GATC_A
Cuts at: 0 573 1773
Size: 573 1200
BglII A'GATC_T
Cuts at: 0 1002 1773
Size: 1002 771
Bme1580I G_kGCm'C
Cuts at: 0 384 565 1773
Size: 384 181 1208
Fragments arranged by size:
1208 384 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 516 559 1773
Size: 516 43 1214
Fragments arranged by size:
1214 516 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 253 997 1498 1773
Size: 72 181 744 501 275
Fragments arranged by size:
744 501 275 181 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 1611 1773
Size: 434 1177 162
Fragments arranged by size:
1177 434 162
BsaHI Gr'CG_yC
Cuts at: 0 31 1773
Size: 31 1742
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1349 1379 1393 1423 1526 1556 1773
Size: 1349 30 14 30 103 30 217
Fragments arranged by size:
1349 217 103 30 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1115 1298 1495 1706 1773
Size: 1115 183 197 211 67
Fragments arranged by size:
1115 211 197 183 67
BseYI C'CCAG_C
Cuts at: 0 1377 1773
Size: 1377 396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1773
Size: 1387 386
BsiHKAI G_wGCw'C
Cuts at: 0 384 1301 1518 1773
Size: 384 917 217 255
Fragments arranged by size:
917 384 255 217
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1352 1773
Size: 1352 421
Bsp1286I G_dGCh'C
Cuts at: 0 384 565 1301 1518 1773
Size: 384 181 736 217 255
Fragments arranged by size:
736 384 255 217 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 580 1773
Size: 580 1193
BsrDI GCAATG_nn'
Cuts at: 0 426 732 1773
Size: 426 306 1041
Fragments arranged by size:
1041 426 306
BsrFI r'CCGG_y
Cuts at: 0 33 1773
Size: 33 1740
BssSI C'ACGA_G
Cuts at: 0 621 1773
Size: 621 1152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1531 1773
Size: 1531 242
BstYI r'GATC_y
Cuts at: 0 1002 1582 1773
Size: 1002 580 191
Fragments arranged by size:
1002 580 191
BtgI C'CryG_G
Cuts at: 0 285 1091 1253 1773
Size: 285 806 162 520
Fragments arranged by size:
806 520 285 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 375 1773
Size: 375 1398
BtsI GCAGTG_nn'
Cuts at: 0 1705 1773
Size: 1705 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 417 452 1416 1451 1773
Size: 417 35 964 35 322
Fragments arranged by size:
964 417 322 35 35
DraI TTT'AAA
Cuts at: 0 774 1149 1773
Size: 774 375 624
Fragments arranged by size:
774 624 375
DraIII CAC_nnn'GTG
Cuts at: 0 1561 1773
Size: 1561 212
DrdI GACnn_nn'nnGTC
Cuts at: 0 316 1270 1773
Size: 316 954 503
Fragments arranged by size:
954 503 316
EaeI y'GGCC_r
Cuts at: 0 524 1773
Size: 524 1249
EarI CTCTTCn'nnn_
Cuts at: 0 1012 1163 1773
Size: 1012 151 610
Fragments arranged by size:
1012 610 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1351 1773
Size: 1351 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 253 937 997 1498 1736 1773
Size: 72 181 684 60 501 238 37
Fragments arranged by size:
684 501 238 181 72 60 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1345 1773
Size: 1345 428
EcoO109I rG'GnC_Cy
Cuts at: 0 561 1022 1744 1773
Size: 561 461 722 29
Fragments arranged by size:
722 561 461 29
EcoRI G'AATT_C
Cuts at: 0 6 682 1773
Size: 6 676 1091
Fragments arranged by size:
1091 676 6
HaeII r_GCGC'y
Cuts at: 0 34 1773
Size: 34 1739
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 688 720 1526 1558 1773
Size: 688 32 806 32 215
Fragments arranged by size:
806 688 215 32 32
HincII GTy'rAC
Cuts at: 0 129 1773
Size: 129 1644
KasI G'GCGC_C
Cuts at: 0 30 1773
Size: 30 1743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1560 1773
Size: 1560 213
MscI TGG'CCA
Cuts at: 0 526 1773
Size: 526 1247
MslI CAynn'nnrTG
Cuts at: 0 1130 1255 1773
Size: 1130 125 518
Fragments arranged by size:
1130 518 125
NarI GG'CG_CC
Cuts at: 0 31 1773
Size: 31 1742
NcoI C'CATG_G
Cuts at: 0 285 1091 1253 1773
Size: 285 806 162 520
Fragments arranged by size:
806 520 285 162
NspI r_CATG'y
Cuts at: 0 227 883 1773
Size: 227 656 890
Fragments arranged by size:
890 656 227
PasI CC'CwG_GG
Cuts at: 0 63 1773
Size: 63 1710
PciI A'CATG_T
Cuts at: 0 223 1773
Size: 223 1550
PflMI CCAn_nnn'nTGG
Cuts at: 0 17 1773
Size: 17 1756
PfoI T'CCnGG_A
Cuts at: 0 10 1773
Size: 10 1763
PpuMI rG'GwC_Cy
Cuts at: 0 1022 1773
Size: 1022 751
PshAI GACnn'nnGTC
Cuts at: 0 233 1773
Size: 233 1540
PsiI TTA'TAA
Cuts at: 0 476 1773
Size: 476 1297
PspOMI G'GGCC_C
Cuts at: 0 561 1773
Size: 561 1212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1722 1754 1773
Size: 1722 32 19
Fragments arranged by size:
1722 32 19
SanDI GG'GwC_CC
Cuts at: 0 1022 1773
Size: 1022 751
SfcI C'TryA_G
Cuts at: 0 462 1196 1773
Size: 462 734 577
Fragments arranged by size:
734 577 462
SfoI GGC'GCC
Cuts at: 0 32 1773
Size: 32 1741
SgrAI Cr'CCGG_yG
Cuts at: 0 33 1773
Size: 33 1740
SpeI A'CTAG_T
Cuts at: 0 1416 1773
Size: 1416 357
SphI G_CATG'C
Cuts at: 0 883 1773
Size: 883 890
SspI AAT'ATT
Cuts at: 0 788 1773
Size: 788 985
StuI AGG'CCT
Cuts at: 0 1592 1773
Size: 1592 181
StyI C'CwwG_G
Cuts at: 0 285 669 1091 1253 1524 1587 1773
Size: 285 384 422 162 271 63 186
Fragments arranged by size:
422 384 285 271 186 162 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1177 1773
Size: 1177 596
TatI w'GTAC_w
Cuts at: 0 980 1370 1773
Size: 980 390 403
Fragments arranged by size:
980 403 390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 210 242 1349 1381 1773
Size: 210 32 1107 32 392
Fragments arranged by size:
1107 392 210 32 32
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI BamHI BanI BanII BbeI BbsI BclI
BglII Bme1580I BmrI BpmI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII KasI MmeI MscI MslI
NarI NcoI NspI PasI PciI PflMI PfoI PpuMI
PshAI PsiI PspOMI PsrI SanDI SfcI SfoI SgrAI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI
BciVI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI EagI EcoICRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SmaI SmlI SnaBI SrfI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI