Restriction map for
pFN21AE2012
(Linear) (Six-base) MAPSORT of: KIEE2012.seq Check: 1725 from: 1 to: 1671
KIEE2012.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 37 350 1671
Size: 37 313 1321
Fragments arranged by size:
1321 313 37
AflIII A'CryG_T
Cuts at: 0 1307 1671
Size: 1307 364
AhdI GACnn_n'nnGTC
Cuts at: 0 33 1671
Size: 33 1638
AleI CACnn'nnGTG
Cuts at: 0 1320 1671
Size: 1320 351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 814 846 1671
Size: 814 32 825
Fragments arranged by size:
825 814 32
ApaI G_GGCC'C
Cuts at: 0 528 587 878 1671
Size: 528 59 291 793
Fragments arranged by size:
793 528 291 59
ApaLI G'TGCA_C
Cuts at: 0 1226 1471 1671
Size: 1226 245 200
Fragments arranged by size:
1226 245 200
AvaI C'yCGr_G
Cuts at: 0 326 886 1033 1337 1564 1671
Size: 326 560 147 304 227 107
Fragments arranged by size:
560 326 304 227 147 107
BamHI G'GATC_C
Cuts at: 0 1072 1671
Size: 1072 599
BanI G'GyrC_C
Cuts at: 0 1028 1205 1609 1671
Size: 1028 177 404 62
Fragments arranged by size:
1028 404 177 62
BanII G_rGCy'C
Cuts at: 0 528 535 587 679 878 1671
Size: 528 7 52 92 199 793
Fragments arranged by size:
793 528 199 92 52 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 853 1027 1671
Size: 853 174 644
Fragments arranged by size:
853 644 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1422 1456 1671
Size: 1422 34 215
Fragments arranged by size:
1422 215 34
BglI GCCn_nnn'nGGC
Cuts at: 0 22 1671
Size: 22 1649
BlpI GC'TnA_GC
Cuts at: 0 380 1671
Size: 380 1291
Bme1580I G_kGCm'C
Cuts at: 0 528 587 686 878 1230 1475 1612 1671
Size: 528 59 99 192 352 245 137 59
Fragments arranged by size:
528 352 245 192 137 99 59 59
BmgBI CAC'GTC
Cuts at: 0 348 407 636 1025 1202 1671
Size: 348 59 229 389 177 469
Fragments arranged by size:
469 389 348 229 177 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 638 1671
Size: 638 1033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 519 1671
Size: 519 1152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 649 691 1369 1671
Size: 252 397 42 678 302
Fragments arranged by size:
678 397 302 252 42
Bpu10I CC'TnA_GC
Cuts at: 0 529 605 1671
Size: 529 76 1066
Fragments arranged by size:
1066 529 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1576 1642 1671
Size: 489 1087 66 29
Fragments arranged by size:
1087 489 66 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 1671
Size: 76 1595
BsaHI Gr'CG_yC
Cuts at: 0 14 1671
Size: 14 1657
BsaWI w'CCGG_w
Cuts at: 0 1196 1671
Size: 1196 475
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1120 1150 1671
Size: 1120 30 521
Fragments arranged by size:
1120 521 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 352 355 768 1078 1671
Size: 94 258 3 413 310 593
Fragments arranged by size:
593 413 310 258 94 3
BseYI C'CCAG_C
Cuts at: 0 211 580 685 909 939 1671
Size: 211 369 105 224 30 732
Fragments arranged by size:
732 369 224 211 105 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1032 1671
Size: 1032 639
BsiEI CG_ry'CG
Cuts at: 0 438 1530 1671
Size: 438 1092 141
Fragments arranged by size:
1092 438 141
BsiHKAI G_wGCw'C
Cuts at: 0 325 347 406 679 1040 1230 1448 1475
Size: 325 22 59 273 361 190 218 27
Cuts at: 1475 1571 1671
Size: 96 100
Fragments arranged by size:
361 325 273 218 190 100 96 59
27 22
BsmI GAATG_Cn'
Cuts at: 0 1414 1671
Size: 1414 257
Bsp1286I G_dGCh'C
Cuts at: 0 325 347 406 528 535 587 679 686
Size: 325 22 59 122 7 52 92 7
Cuts at: 686 878 1040 1230 1448 1475 1571 1612 1671
Size: 192 162 190 218 27 96 41 59
Fragments arranged by size:
325 218 192 190 162 122 96 92
59 59 52 41 27 22 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 990 1671
Size: 990 681
BsrBI CCG'CTC
Cuts at: 0 1092 1485 1671
Size: 1092 393 186
Fragments arranged by size:
1092 393 186
BsrFI r'CCGG_y
Cuts at: 0 1525 1671
Size: 1525 146
BsrGI T'GTAC_A
Cuts at: 0 462 1671
Size: 462 1209
BssSI C'ACGA_G
Cuts at: 0 1264 1480 1671
Size: 1264 216 191
Fragments arranged by size:
1264 216 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1055 1671
Size: 1055 616
BstXI CCAn_nnnn'nTGG
Cuts at: 0 795 1671
Size: 795 876
BstYI r'GATC_y
Cuts at: 0 1072 1671
Size: 1072 599
Bsu36I CC'TnA_GG
Cuts at: 0 299 1671
Size: 299 1372
BtgI C'CryG_G
Cuts at: 0 1013 1544 1671
Size: 1013 531 127
Fragments arranged by size:
1013 531 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 975 1671
Size: 975 696
BtsI GCAGTG_nn'
Cuts at: 0 1053 1671
Size: 1053 618
DraIII CAC_nnn'GTG
Cuts at: 0 1234 1379 1671
Size: 1234 145 292
Fragments arranged by size:
1234 292 145
EaeI y'GGCC_r
Cuts at: 0 1527 1547 1671
Size: 1527 20 124
Fragments arranged by size:
1527 124 20
EagI C'GGCC_G
Cuts at: 0 1527 1671
Size: 1527 144
EarI CTCTTCn'nnn_
Cuts at: 0 1138 1669 1671
Size: 1138 531 2
Fragments arranged by size:
1138 531 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 1671
Size: 40 1631
EcoICRI GAG'CTC
Cuts at: 0 677 1671
Size: 677 994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 649 691 1369 1671
Size: 252 397 42 678 302
Fragments arranged by size:
678 397 302 252 42
EcoO109I rG'GnC_Cy
Cuts at: 0 168 524 525 584 624 874 875 974
Size: 168 356 1 59 40 250 1 99
Cuts at: 974 1671
Size: 697
Fragments arranged by size:
697 356 250 168 99 59 40 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1317 1629 1671
Size: 1317 312 42
Fragments arranged by size:
1317 312 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 1057 1089 1671
Size: 19 32 1006 32 582
Fragments arranged by size:
1006 582 32 32 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 317 800 1671
Size: 56 261 483 871
Fragments arranged by size:
871 483 261 56
MscI TGG'CCA
Cuts at: 0 1549 1671
Size: 1549 122
MslI CAynn'nnrTG
Cuts at: 0 397 706 1320 1543 1671
Size: 397 309 614 223 128
Fragments arranged by size:
614 397 309 223 128
MspA1I CmG'CkG
Cuts at: 0 215 379 897 1532 1671
Size: 215 164 518 635 139
Fragments arranged by size:
635 518 215 164 139
NcoI C'CATG_G
Cuts at: 0 1013 1544 1671
Size: 1013 531 127
Fragments arranged by size:
1013 531 127
NspI r_CATG'y
Cuts at: 0 909 1311 1671
Size: 909 402 360
Fragments arranged by size:
909 402 360
PasI CC'CwG_GG
Cuts at: 0 411 840 868 1671
Size: 411 429 28 803
Fragments arranged by size:
803 429 411 28
PciI A'CATG_T
Cuts at: 0 1307 1671
Size: 1307 364
PfoI T'CCnGG_A
Cuts at: 0 1075 1169 1346 1671
Size: 1075 94 177 325
Fragments arranged by size:
1075 325 177 94
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 1671
Size: 1345 32 294
Fragments arranged by size:
1345 294 32
PspOMI G'GGCC_C
Cuts at: 0 524 583 874 1671
Size: 524 59 291 797
Fragments arranged by size:
797 524 291 59
PstI C_TGCA'G
Cuts at: 0 492 923 1671
Size: 492 431 748
Fragments arranged by size:
748 492 431
PvuII CAG'CTG
Cuts at: 0 379 897 1671
Size: 379 518 774
Fragments arranged by size:
774 518 379
SacI G_AGCT'C
Cuts at: 0 679 1671
Size: 679 992
SapI GCTCTTCn'nnn_
Cuts at: 0 1669 1671
Size: 1669 2
SfcI C'TryA_G
Cuts at: 0 351 488 919 1671
Size: 351 137 431 752
Fragments arranged by size:
752 431 351 137
SmaI CCC'GGG
Cuts at: 0 888 1339 1671
Size: 888 451 332
Fragments arranged by size:
888 451 332
SmlI C'TyrA_G
Cuts at: 0 504 1591 1657 1671
Size: 504 1087 66 14
Fragments arranged by size:
1087 504 66 14
SphI G_CATG'C
Cuts at: 0 909 1671
Size: 909 762
SrfI GCCC'GGGC
Cuts at: 0 1339 1671
Size: 1339 332
StuI AGG'CCT
Cuts at: 0 1282 1671
Size: 1282 389
StyI C'CwwG_G
Cuts at: 0 566 1013 1544 1671
Size: 566 447 531 127
Fragments arranged by size:
566 531 447 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 405 766 1066 1332 1671
Size: 322 83 361 300 266 339
Fragments arranged by size:
361 339 322 300 266 83
TatI w'GTAC_w
Cuts at: 0 462 1671
Size: 462 1209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 1671
Size: 128 32 1511
Fragments arranged by size:
1511 128 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1671
Size: 792 879
XmaI C'CCGG_G
Cuts at: 0 886 1337 1671
Size: 886 451 334
Fragments arranged by size:
886 451 334
XmnI GAAnn'nnTTC
Cuts at: 0 1084 1651 1671
Size: 1084 567 20
Fragments arranged by size:
1084 567 20
Enzymes that do cut:
AccI AflIII AhdI AleI AloI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI Hin4I MmeI
MscI MslI MspA1I NcoI NspI PasI PciI PfoI
PpiI PspOMI PstI PvuII SacI SapI SfcI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BglII BmtI BsaAI
BsaBI BsiWI BsmBI BspEI BspHI BsrDI BssHII BstBI
BstEII BstZ17I ClaI CspCI DraI DrdI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI
ZraI