Restriction map for
pFN21AE2011
(Linear) (Six-base) MAPSORT of: KIEE2011.seq Check: 6662 from: 1 to: 1770
KIEE2011.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 923 1770
Size: 923 847
Acc65I G'GTAC_C
Cuts at: 0 1591 1770
Size: 1591 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 832 1326 1770
Size: 121 711 494 444
Fragments arranged by size:
711 494 444 121
AfeI AGC'GCT
Cuts at: 0 646 1363 1770
Size: 646 717 407
Fragments arranged by size:
717 646 407
AflIII A'CryG_T
Cuts at: 0 1574 1770
Size: 1574 196
AgeI A'CCGG_T
Cuts at: 0 537 1770
Size: 537 1233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1282 1770
Size: 1282 488
AlwNI CAG_nnn'CTG
Cuts at: 0 249 1293 1770
Size: 249 1044 477
Fragments arranged by size:
1044 477 249
ApaLI G'TGCA_C
Cuts at: 0 434 1770
Size: 434 1336
AvaI C'yCGr_G
Cuts at: 0 712 1009 1770
Size: 712 297 761
Fragments arranged by size:
761 712 297
BanI G'GyrC_C
Cuts at: 0 1591 1770
Size: 1591 179
BanII G_rGCy'C
Cuts at: 0 1096 1516 1770
Size: 1096 420 254
Fragments arranged by size:
1096 420 254
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 905 1770
Size: 134 771 865
Fragments arranged by size:
865 771 134
BglI GCCn_nnn'nGGC
Cuts at: 0 1037 1770
Size: 1037 733
BglII A'GATC_T
Cuts at: 0 930 1605 1770
Size: 930 675 165
Fragments arranged by size:
930 675 165
Bme1580I G_kGCm'C
Cuts at: 0 438 1042 1770
Size: 438 604 728
Fragments arranged by size:
728 604 438
BmgBI CAC'GTC
Cuts at: 0 1428 1770
Size: 1428 342
BmrI ACTGGGnnnn_n'
Cuts at: 0 301 1113 1770
Size: 301 812 657
Fragments arranged by size:
812 657 301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 1464 1770
Size: 1239 225 306
Fragments arranged by size:
1239 306 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 263 496 1043 1225 1636 1770
Size: 250 13 233 547 182 411 134
Fragments arranged by size:
547 411 250 233 182 134 13
Bpu10I CC'TnA_GC
Cuts at: 0 1090 1770
Size: 1090 680
BsaI GGTCTCn'nnnn_
Cuts at: 0 381 754 1010 1770
Size: 381 373 256 760
Fragments arranged by size:
760 381 373 256
BsaHI Gr'CG_yC
Cuts at: 0 1463 1770
Size: 1463 307
BsaWI w'CCGG_w
Cuts at: 0 537 1626 1759 1770
Size: 537 1089 133 11
Fragments arranged by size:
1089 537 133 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 1204 1219 1234 1249 1264 1480
Size: 361 30 813 15 15 15 15 216
Cuts at: 1480 1510 1770
Size: 30 260
Fragments arranged by size:
813 361 260 216 30 30 15 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 865 868 1198 1228 1255 1321 1770
Size: 403 462 3 330 30 27 66 449
Fragments arranged by size:
462 449 403 330 66 30 27 3
BseYI C'CCAG_C
Cuts at: 0 1293 1699 1770
Size: 1293 406 71
Fragments arranged by size:
1293 406 71
BsiEI CG_ry'CG
Cuts at: 0 1032 1161 1770
Size: 1032 129 609
Fragments arranged by size:
1032 609 129
BsiHKAI G_wGCw'C
Cuts at: 0 438 1770
Size: 438 1332
BsmI GAATG_Cn'
Cuts at: 0 1561 1770
Size: 1561 209
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1201 1770
Size: 1201 569
Bsp1286I G_dGCh'C
Cuts at: 0 438 1042 1096 1516 1770
Size: 438 604 54 420 254
Fragments arranged by size:
604 438 420 254 54
BspEI T'CCGG_A
Cuts at: 0 1626 1770
Size: 1626 144
BspHI T'CATG_A
Cuts at: 0 794 1770
Size: 794 976
BsrBI CCG'CTC
Cuts at: 0 858 1770
Size: 858 912
BsrFI r'CCGG_y
Cuts at: 0 537 1770
Size: 537 1233
BssSI C'ACGA_G
Cuts at: 0 448 1770
Size: 448 1322
BstEII G'GTnAC_C
Cuts at: 0 540 805 1770
Size: 540 265 965
Fragments arranged by size:
965 540 265
BstYI r'GATC_y
Cuts at: 0 707 881 930 977 1004 1605 1770
Size: 707 174 49 47 27 601 165
Fragments arranged by size:
707 601 174 165 49 47 27
Bsu36I CC'TnA_GG
Cuts at: 0 457 1770
Size: 457 1313
BtgI C'CryG_G
Cuts at: 0 257 620 686 1026 1155 1508 1528 1770
Size: 257 363 66 340 129 353 20 242
Fragments arranged by size:
363 353 340 257 242 129 66 20
DrdI GACnn_nn'nnGTC
Cuts at: 0 1198 1770
Size: 1198 572
EaeI y'GGCC_r
Cuts at: 0 20 363 966 1029 1158 1770
Size: 20 343 603 63 129 612
Fragments arranged by size:
612 603 343 129 63 20
EagI C'GGCC_G
Cuts at: 0 1029 1158 1770
Size: 1029 129 612
Fragments arranged by size:
1029 612 129
EarI CTCTTCn'nnn_
Cuts at: 0 652 1666 1770
Size: 652 1014 104
Fragments arranged by size:
1014 652 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 71 1770
Size: 71 1699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 250 263 496 832 1043 1225 1326
Size: 121 129 13 233 336 211 182 101
Cuts at: 1326 1636 1770
Size: 310 134
Fragments arranged by size:
336 310 233 211 182 134 129 121
101 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 715 1341 1770
Size: 715 626 429
Fragments arranged by size:
715 626 429
EcoO109I rG'GnC_Cy
Cuts at: 0 1065 1107 1200 1770
Size: 1065 42 93 570
Fragments arranged by size:
1065 570 93 42
HaeII r_GCGC'y
Cuts at: 0 648 1365 1770
Size: 648 717 405
Fragments arranged by size:
717 648 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 1007 1039 1129 1161 1234 1266
Size: 361 32 614 32 90 32 73 32
Cuts at: 1266 1480 1512 1770
Size: 214 32 258
Fragments arranged by size:
614 361 258 214 90 73 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 723 741 1770
Size: 723 18 1029
Fragments arranged by size:
1029 723 18
KpnI G_GTAC'C
Cuts at: 0 1595 1770
Size: 1595 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1361 1770
Size: 506 855 409
Fragments arranged by size:
855 506 409
MscI TGG'CCA
Cuts at: 0 22 365 968 1770
Size: 22 343 603 802
Fragments arranged by size:
802 603 343 22
MspA1I CmG'CkG
Cuts at: 0 1028 1157 1770
Size: 1028 129 613
Fragments arranged by size:
1028 613 129
NcoI C'CATG_G
Cuts at: 0 686 1528 1770
Size: 686 842 242
Fragments arranged by size:
842 686 242
NotI GC'GGCC_GC
Cuts at: 0 1158 1770
Size: 1158 612
NspI r_CATG'y
Cuts at: 0 495 1770
Size: 495 1275
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 1529 1770
Size: 372 1157 241
Fragments arranged by size:
1157 372 241
PfoI T'CCnGG_A
Cuts at: 0 248 1202 1770
Size: 248 954 568
Fragments arranged by size:
954 568 248
PpuMI rG'GwC_Cy
Cuts at: 0 1065 1200 1770
Size: 1065 135 570
Fragments arranged by size:
1065 570 135
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 907 939 1770
Size: 907 32 831
Fragments arranged by size:
907 831 32
PstI C_TGCA'G
Cuts at: 0 163 1320 1770
Size: 163 1157 450
Fragments arranged by size:
1157 450 163
SacII CC_GC'GG
Cuts at: 0 1029 1158 1770
Size: 1029 129 612
Fragments arranged by size:
1029 612 129
SexAI A'CCwGG_T
Cuts at: 0 371 1770
Size: 371 1399
SfcI C'TryA_G
Cuts at: 0 159 400 1316 1770
Size: 159 241 916 454
Fragments arranged by size:
916 454 241 159
SmlI C'TyrA_G
Cuts at: 0 712 1770
Size: 712 1058
StuI AGG'CCT
Cuts at: 0 222 1770
Size: 222 1548
StyI C'CwwG_G
Cuts at: 0 686 764 1110 1528 1770
Size: 686 78 346 418 242
Fragments arranged by size:
686 418 346 242 78
TatI w'GTAC_w
Cuts at: 0 1220 1415 1770
Size: 1220 195 355
Fragments arranged by size:
1220 355 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 428 460 760 792 1219 1251 1770
Size: 428 32 300 32 427 32 519
Fragments arranged by size:
519 428 427 300 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 373 1770
Size: 373 1397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 1770
Size: 225 1545
XhoI C'TCGA_G
Cuts at: 0 712 1770
Size: 712 1058
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AgeI AlfI AlwNI
ApaLI AvaI BanI BanII BbsI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrFI BssSI BstEII BstYI Bsu36I
BtgI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MspA1I
NcoI NotI NspI PflMI PfoI PpuMI PsrI PstI
SacII SexAI SfcI SmlI StuI StyI TatI TstI
Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AclI AflII AhdI AleI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BpuEI
BsaAI BsaBI BsgI BsiWI BspMI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NheI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII XbaI XmaI XmnI
ZraI