Restriction map for
pFN21AE1992
(Linear) (Six-base) MAPSORT of: KIEE1992.seq Check: 7620 from: 1 to: 1713
KIEE1992.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
Acc65I G'GTAC_C
Cuts at: 0 1283 1713
Size: 1283 430
AclI AA'CG_TT
Cuts at: 0 1214 1713
Size: 1214 499
AflII C'TTAA_G
Cuts at: 0 478 1713
Size: 478 1235
AflIII A'CryG_T
Cuts at: 0 65 1074 1076 1100 1713
Size: 65 1009 2 24 613
Fragments arranged by size:
1009 613 65 24 2
AleI CACnn'nnGTG
Cuts at: 0 415 1713
Size: 415 1298
AlwNI CAG_nnn'CTG
Cuts at: 0 677 1713
Size: 677 1036
ApoI r'AATT_y
Cuts at: 0 1179 1713
Size: 1179 534
AseI AT'TA_AT
Cuts at: 0 767 1713
Size: 767 946
AvaI C'yCGr_G
Cuts at: 0 72 737 1107 1713
Size: 72 665 370 606
Fragments arranged by size:
665 606 370 72
BanI G'GyrC_C
Cuts at: 0 112 1283 1506 1713
Size: 112 1171 223 207
Fragments arranged by size:
1171 223 207 112
BanII G_rGCy'C
Cuts at: 0 555 707 1516 1713
Size: 555 152 809 197
Fragments arranged by size:
809 555 197 152
BbeI G_GCGC'C
Cuts at: 0 116 1713
Size: 116 1597
BbvCI CC'TCA_GC
Cuts at: 0 1591 1713
Size: 1591 122
BclI T'GATC_A
Cuts at: 0 422 1713
Size: 422 1291
BglI GCCn_nnn'nGGC
Cuts at: 0 448 1713
Size: 448 1265
BglII A'GATC_T
Cuts at: 0 1010 1713
Size: 1010 703
Bme1580I G_kGCm'C
Cuts at: 0 1509 1713
Size: 1509 204
BmrI ACTGGGnnnn_n'
Cuts at: 0 883 970 1713
Size: 883 87 743
Fragments arranged by size:
883 743 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 625 1599 1713
Size: 625 974 114
Fragments arranged by size:
974 625 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 721 1066 1713
Size: 200 521 345 647
Fragments arranged by size:
647 521 345 200
Bpu10I CC'TnA_GC
Cuts at: 0 860 1249 1591 1648 1713
Size: 860 389 342 57 65
Fragments arranged by size:
860 389 342 65 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 1713
Size: 199 1514
BsaAI yAC'GTr
Cuts at: 0 1077 1599 1713
Size: 1077 522 114
Fragments arranged by size:
1077 522 114
BsaHI Gr'CG_yC
Cuts at: 0 113 856 1713
Size: 113 743 857
Fragments arranged by size:
857 743 113
BsaWI w'CCGG_w
Cuts at: 0 1082 1713
Size: 1082 631
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 198 228 1713
Size: 198 30 1485
Fragments arranged by size:
1485 198 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1010 1713
Size: 1010 703
BseYI C'CCAG_C
Cuts at: 0 48 1426 1713
Size: 48 1378 287
Fragments arranged by size:
1378 287 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1080 1713
Size: 1080 633
BsiHKAI G_wGCw'C
Cuts at: 0 695 1713
Size: 695 1018
Bsp1286I G_dGCh'C
Cuts at: 0 555 695 707 1509 1516 1713
Size: 555 140 12 802 7 197
Fragments arranged by size:
802 555 197 140 12 7
BspEI T'CCGG_A
Cuts at: 0 1082 1713
Size: 1082 631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1712 1713
Size: 1712 1
BsrBI CCG'CTC
Cuts at: 0 278 1640 1713
Size: 278 1362 73
Fragments arranged by size:
1362 278 73
BsrDI GCAATG_nn'
Cuts at: 0 183 540 1713
Size: 183 357 1173
Fragments arranged by size:
1173 357 183
BsrFI r'CCGG_y
Cuts at: 0 115 405 1713
Size: 115 290 1308
Fragments arranged by size:
1308 290 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1613 1713
Size: 1613 100
BstBI TT'CG_AA
Cuts at: 0 336 1713
Size: 336 1377
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 1713
Size: 417 1296
BstYI r'GATC_y
Cuts at: 0 399 1010 1713
Size: 399 611 703
Fragments arranged by size:
703 611 399
Bsu36I CC'TnA_GG
Cuts at: 0 542 1713
Size: 542 1171
BtgI C'CryG_G
Cuts at: 0 464 1713
Size: 464 1249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 1713
Size: 39 1674
BtsI GCAGTG_nn'
Cuts at: 0 394 675 1036 1122 1202 1690 1713
Size: 394 281 361 86 80 488 23
Fragments arranged by size:
488 394 361 281 86 80 23
ClaI AT'CG_AT
Cuts at: 0 758 1713
Size: 758 955
EaeI y'GGCC_r
Cuts at: 0 92 232 407 1631 1713
Size: 92 140 175 1224 82
Fragments arranged by size:
1224 175 140 92 82
EarI CTCTTCn'nnn_
Cuts at: 0 179 1713
Size: 179 1534
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 739 1713
Size: 739 974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 721 1066 1713
Size: 200 521 345 647
Fragments arranged by size:
647 521 345 200
EcoO109I rG'GnC_Cy
Cuts at: 0 288 654 872 1343 1644 1713
Size: 288 366 218 471 301 69
Fragments arranged by size:
471 366 301 288 218 69
HaeII r_GCGC'y
Cuts at: 0 12 116 1713
Size: 12 104 1597
Fragments arranged by size:
1597 104 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 198 230 1713
Size: 198 32 1483
Fragments arranged by size:
1483 198 32
HindIII A'AGCT_T
Cuts at: 0 251 331 1713
Size: 251 80 1382
Fragments arranged by size:
1382 251 80
KasI G'GCGC_C
Cuts at: 0 112 1713
Size: 112 1601
KpnI G_GTAC'C
Cuts at: 0 1287 1713
Size: 1287 426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1383 1713
Size: 240 1143 330
Fragments arranged by size:
1143 330 240
MscI TGG'CCA
Cuts at: 0 234 1713
Size: 234 1479
MslI CAynn'nnrTG
Cuts at: 0 176 415 517 769 979 1713
Size: 176 239 102 252 210 734
Fragments arranged by size:
734 252 239 210 176 102
MspA1I CmG'CkG
Cuts at: 0 381 1265 1713
Size: 381 884 448
Fragments arranged by size:
884 448 381
NaeI GCC'GGC
Cuts at: 0 117 407 1713
Size: 117 290 1306
Fragments arranged by size:
1306 290 117
NarI GG'CG_CC
Cuts at: 0 113 1713
Size: 113 1600
NgoMIV G'CCGG_C
Cuts at: 0 115 405 1713
Size: 115 290 1308
Fragments arranged by size:
1308 290 115
NspI r_CATG'y
Cuts at: 0 69 1713
Size: 69 1644
PciI A'CATG_T
Cuts at: 0 65 1713
Size: 65 1648
PfoI T'CCnGG_A
Cuts at: 0 665 710 1713
Size: 665 45 1003
Fragments arranged by size:
1003 665 45
PmlI CAC'GTG
Cuts at: 0 1077 1599 1713
Size: 1077 522 114
Fragments arranged by size:
1077 522 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1713
Size: 1000 32 681
Fragments arranged by size:
1000 681 32
PpuMI rG'GwC_Cy
Cuts at: 0 288 654 872 1713
Size: 288 366 218 841
Fragments arranged by size:
841 366 288 218
PshAI GACnn'nnGTC
Cuts at: 0 271 374 466 1713
Size: 271 103 92 1247
Fragments arranged by size:
1247 271 103 92
PsiI TTA'TAA
Cuts at: 0 1522 1713
Size: 1522 191
PstI C_TGCA'G
Cuts at: 0 1532 1709 1713
Size: 1532 177 4
Fragments arranged by size:
1532 177 4
PvuII CAG'CTG
Cuts at: 0 1265 1713
Size: 1265 448
SexAI A'CCwGG_T
Cuts at: 0 314 1713
Size: 314 1399
SfcI C'TryA_G
Cuts at: 0 528 1528 1705 1713
Size: 528 1000 177 8
Fragments arranged by size:
1000 528 177 8
SfoI GGC'GCC
Cuts at: 0 114 1713
Size: 114 1599
SmaI CCC'GGG
Cuts at: 0 1109 1713
Size: 1109 604
SmlI C'TyrA_G
Cuts at: 0 478 1713
Size: 478 1235
SspI AAT'ATT
Cuts at: 0 1230 1713
Size: 1230 483
StuI AGG'CCT
Cuts at: 0 608 1713
Size: 608 1105
StyI C'CwwG_G
Cuts at: 0 650 1713
Size: 650 1063
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 765 1713
Size: 33 732 948
Fragments arranged by size:
948 732 33
TatI w'GTAC_w
Cuts at: 0 68 1713
Size: 68 1645
XmaI C'CCGG_G
Cuts at: 0 1107 1713
Size: 1107 606
Enzymes that do cut:
Acc65I AclI AflII AflIII AleI AlwNI ApoI AseI
AvaI BanI BanII BbeI BbvCI BclI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmlI PpiI PpuMI PshAI PsiI PstI PvuII
SexAI SfcI SfoI SmaI SmlI SspI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AcuI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BfrBI BlpI BmgBI BmtI BpuEI
BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssHII
BssSI BstEII BstZ17I CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI