(Linear) (Six-base) MAPSORT of: KIEE1990.seq Check: 2994 from: 1 to: 1701 KIEE1990.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AccI GT'mk_AC Cuts at: 0 1031 1701 Size: 1031 670 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 463 658 1701 Size: 274 189 195 1043 Fragments arranged by size: 1043 274 195 189 AfeI AGC'GCT Cuts at: 0 304 1701 Size: 304 1397 AflIII A'CryG_T Cuts at: 0 458 1701 Size: 458 1243 AlwNI CAG_nnn'CTG Cuts at: 0 390 1275 1317 1701 Size: 390 885 42 384 Fragments arranged by size: 885 390 384 42 ApaI G_GGCC'C Cuts at: 0 89 195 1542 1616 1701 Size: 89 106 1347 74 85 Fragments arranged by size: 1347 106 89 85 74 ApoI r'AATT_y Cuts at: 0 1588 1701 Size: 1588 113 AvaI C'yCGr_G Cuts at: 0 643 1701 Size: 643 1058 BamHI G'GATC_C Cuts at: 0 1669 1701 Size: 1669 32 BanI G'GyrC_C Cuts at: 0 1455 1701 Size: 1455 246 BanII G_rGCy'C Cuts at: 0 89 195 397 449 787 1234 1300 1542 Size: 89 106 202 52 338 447 66 242 Cuts at: 1542 1616 1701 Size: 74 85 Fragments arranged by size: 447 338 242 202 106 89 85 74 66 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 527 1701 Size: 493 34 1174 Fragments arranged by size: 1174 493 34 BglI GCCn_nnn'nGGC Cuts at: 0 190 943 1701 Size: 190 753 758 Fragments arranged by size: 758 753 190 BglII A'GATC_T Cuts at: 0 482 1346 1701 Size: 482 864 355 Fragments arranged by size: 864 482 355 BlpI GC'TnA_GC Cuts at: 0 70 391 1701 Size: 70 321 1310 Fragments arranged by size: 1310 321 70 Bme1580I G_kGCm'C Cuts at: 0 89 195 471 1460 1542 1616 1701 Size: 89 106 276 989 82 74 85 Fragments arranged by size: 989 276 106 89 85 82 74 BmrI ACTGGGnnnn_n' Cuts at: 0 134 391 1433 1454 1701 Size: 134 257 1042 21 247 Fragments arranged by size: 1042 257 247 134 21 BmtI G_CTAG'C Cuts at: 0 343 556 1701 Size: 343 213 1145 Fragments arranged by size: 1145 343 213 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1153 1168 1234 1412 1701 Size: 1153 15 66 178 289 Fragments arranged by size: 1153 289 178 66 15 Bpu10I CC'TnA_GC Cuts at: 0 344 1701 Size: 344 1357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1248 1701 Size: 1248 453 BsaI GGTCTCn'nnnn_ Cuts at: 0 1621 1701 Size: 1621 80 BsaHI Gr'CG_yC Cuts at: 0 26 570 614 1701 Size: 26 544 44 1087 Fragments arranged by size: 1087 544 44 26 BsaWI w'CCGG_w Cuts at: 0 854 1701 Size: 854 847 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 361 391 1701 Size: 361 30 1310 Fragments arranged by size: 1310 361 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 184 445 582 685 786 1639 1701 Size: 184 261 137 103 101 853 62 Fragments arranged by size: 853 261 184 137 103 101 62 BseYI C'CCAG_C Cuts at: 0 148 188 225 332 660 741 1615 1655 Size: 148 40 37 107 328 81 874 40 Cuts at: 1655 1701 Size: 46 Fragments arranged by size: 874 328 148 107 81 46 40 40 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1062 1234 1546 1701 Size: 1062 172 312 155 Fragments arranged by size: 1062 312 172 155 BsiEI CG_ry'CG Cuts at: 0 411 454 1701 Size: 411 43 1247 Fragments arranged by size: 1247 411 43 BsiHKAI G_wGCw'C Cuts at: 0 874 1300 1701 Size: 874 426 401 Fragments arranged by size: 874 426 401 Bsp1286I G_dGCh'C Cuts at: 0 89 195 397 449 471 787 874 1234 Size: 89 106 202 52 22 316 87 360 Cuts at: 1234 1300 1460 1542 1616 1701 Size: 66 160 82 74 85 Fragments arranged by size: 360 316 202 160 106 89 87 85 82 74 66 52 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 929 1701 Size: 929 772 BsrBI CCG'CTC Cuts at: 0 207 1701 Size: 207 1494 BsrDI GCAATG_nn' Cuts at: 0 1050 1701 Size: 1050 651 BsrFI r'CCGG_y Cuts at: 0 893 1253 1701 Size: 893 360 448 Fragments arranged by size: 893 448 360 BssHII G'CGCG_C Cuts at: 0 514 1701 Size: 514 1187 BssSI C'ACGA_G Cuts at: 0 618 1701 Size: 618 1083 BstXI CCAn_nnnn'nTGG Cuts at: 0 21 1446 1701 Size: 21 1425 255 Fragments arranged by size: 1425 255 21 BstYI r'GATC_y Cuts at: 0 314 482 989 1278 1346 1669 1701 Size: 314 168 507 289 68 323 32 Fragments arranged by size: 507 323 314 289 168 68 32 BtgI C'CryG_G Cuts at: 0 108 1701 Size: 108 1593 DrdI GACnn_nn'nnGTC Cuts at: 0 1027 1701 Size: 1027 674 EaeI y'GGCC_r Cuts at: 0 182 320 440 451 895 950 1255 1701 Size: 182 138 120 11 444 55 305 446 Fragments arranged by size: 446 444 305 182 138 120 55 11 EagI C'GGCC_G Cuts at: 0 451 1701 Size: 451 1250 EarI CTCTTCn'nnn_ Cuts at: 0 853 1337 1701 Size: 853 484 364 Fragments arranged by size: 853 484 364 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 721 789 1105 1701 Size: 721 68 316 596 Fragments arranged by size: 721 596 316 68 EcoICRI GAG'CTC Cuts at: 0 1298 1701 Size: 1298 403 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 463 658 1153 1168 1234 1412 1701 Size: 274 189 195 495 15 66 178 289 Fragments arranged by size: 495 289 274 195 189 178 66 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 256 1069 1701 Size: 256 813 632 Fragments arranged by size: 813 632 256 EcoO109I rG'GnC_Cy Cuts at: 0 85 86 192 219 700 1056 1538 1554 Size: 85 1 106 27 481 356 482 16 Cuts at: 1554 1572 1612 1701 Size: 18 40 89 Fragments arranged by size: 482 481 356 106 89 85 40 27 18 16 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1242 1701 Size: 1242 459 HaeII r_GCGC'y Cuts at: 0 306 391 1147 1201 1701 Size: 306 85 756 54 500 Fragments arranged by size: 756 500 306 85 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 361 393 947 979 1701 Size: 361 32 554 32 722 Fragments arranged by size: 722 554 361 32 32 MscI TGG'CCA Cuts at: 0 184 952 1701 Size: 184 768 749 Fragments arranged by size: 768 749 184 MspA1I CmG'CkG Cuts at: 0 110 188 225 534 660 684 1535 1599 Size: 110 78 37 309 126 24 851 64 Cuts at: 1599 1701 Size: 102 Fragments arranged by size: 851 309 126 110 102 78 64 37 24 NaeI GCC'GGC Cuts at: 0 895 1701 Size: 895 806 NgoMIV G'CCGG_C Cuts at: 0 893 1701 Size: 893 808 NheI G'CTAG_C Cuts at: 0 339 552 1701 Size: 339 213 1149 Fragments arranged by size: 1149 339 213 NspI r_CATG'y Cuts at: 0 462 981 1701 Size: 462 519 720 Fragments arranged by size: 720 519 462 PasI CC'CwG_GG Cuts at: 0 90 1701 Size: 90 1611 PciI A'CATG_T Cuts at: 0 458 1701 Size: 458 1243 PflMI CCAn_nnn'nTGG Cuts at: 0 1317 1701 Size: 1317 384 PfoI T'CCnGG_A Cuts at: 0 1064 1701 Size: 1064 637 PpuMI rG'GwC_Cy Cuts at: 0 219 700 1572 1701 Size: 219 481 872 129 Fragments arranged by size: 872 481 219 129 PspOMI G'GGCC_C Cuts at: 0 85 191 1538 1612 1701 Size: 85 106 1347 74 89 Fragments arranged by size: 1347 106 89 85 74 PstI C_TGCA'G Cuts at: 0 1193 1205 1598 1701 Size: 1193 12 393 103 Fragments arranged by size: 1193 393 103 12 PvuII CAG'CTG Cuts at: 0 188 684 1599 1701 Size: 188 496 915 102 Fragments arranged by size: 915 496 188 102 SacI G_AGCT'C Cuts at: 0 1300 1701 Size: 1300 401 SacII CC_GC'GG Cuts at: 0 111 1701 Size: 111 1590 SapI GCTCTTCn'nnn_ Cuts at: 0 853 1701 Size: 853 848 ScaI AGT'ACT Cuts at: 0 907 1701 Size: 907 794 SexAI A'CCwGG_T Cuts at: 0 1034 1574 1701 Size: 1034 540 127 Fragments arranged by size: 1034 540 127 SfcI C'TryA_G Cuts at: 0 1189 1201 1594 1701 Size: 1189 12 393 107 Fragments arranged by size: 1189 393 107 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 190 1701 Size: 190 1511 SmaI CCC'GGG Cuts at: 0 645 1701 Size: 645 1056 SmlI C'TyrA_G Cuts at: 0 1227 1701 Size: 1227 474 StuI AGG'CCT Cuts at: 0 295 418 1007 1502 1701 Size: 295 123 589 495 199 Fragments arranged by size: 589 495 295 199 123 StyI C'CwwG_G Cuts at: 0 813 864 941 1608 1701 Size: 813 51 77 667 93 Fragments arranged by size: 813 667 93 77 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 652 1432 1701 Size: 652 780 269 Fragments arranged by size: 780 652 269 TatI w'GTAC_w Cuts at: 0 905 1701 Size: 905 796 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 39 1701 Size: 39 1662 XmaI C'CCGG_G Cuts at: 0 643 1701 Size: 643 1058 XmnI GAAnn'nnTTC Cuts at: 0 262 1701 Size: 262 1439 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApaI ApoI AvaI BamHI BanI BanII BcgI BglI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MscI MspA1I NaeI NgoMIV NheI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfiI SmaI SmlI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI BmgBI BplI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NarI NcoI NdeI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI ZraI