Restriction map for
pFN21AE1984
(Linear) (Six-base) MAPSORT of: KIEE1984.seq Check: 1537 from: 1 to: 1677
KIEE1984.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1386 1677
Size: 988 398 291
Fragments arranged by size:
988 398 291
AflII C'TTAA_G
Cuts at: 0 246 1677
Size: 246 1431
AflIII A'CryG_T
Cuts at: 0 1573 1677
Size: 1573 104
AleI CACnn'nnGTG
Cuts at: 0 653 1632 1677
Size: 653 979 45
Fragments arranged by size:
979 653 45
ApoI r'AATT_y
Cuts at: 0 163 532 841 918 1677
Size: 163 369 309 77 759
Fragments arranged by size:
759 369 309 163 77
BamHI G'GATC_C
Cuts at: 0 53 1677
Size: 53 1624
BanII G_rGCy'C
Cuts at: 0 718 1677
Size: 718 959
BbsI GAAGACnn'nnnn_
Cuts at: 0 284 1677
Size: 284 1393
BciVI GTATCCnnnnn_n'
Cuts at: 0 1521 1677
Size: 1521 156
BclI T'GATC_A
Cuts at: 0 180 345 1677
Size: 180 165 1332
Fragments arranged by size:
1332 180 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1610 1677
Size: 1610 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 140 395 1677
Size: 29 111 255 1282
Fragments arranged by size:
1282 255 111 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1084 1580 1677
Size: 1055 29 496 97
Fragments arranged by size:
1055 496 97 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 995 1324 1677
Size: 995 329 353
Fragments arranged by size:
995 353 329
BsaBI GATnn'nnATC
Cuts at: 0 227 1677
Size: 227 1450
Bsp1286I G_dGCh'C
Cuts at: 0 718 1677
Size: 718 959
BspHI T'CATG_A
Cuts at: 0 348 1677
Size: 348 1329
BsrDI GCAATG_nn'
Cuts at: 0 466 1394 1677
Size: 466 928 283
Fragments arranged by size:
928 466 283
BsrFI r'CCGG_y
Cuts at: 0 1350 1677
Size: 1350 327
BstYI r'GATC_y
Cuts at: 0 53 1677
Size: 53 1624
BtgI C'CryG_G
Cuts at: 0 1130 1182 1677
Size: 1130 52 495
Fragments arranged by size:
1130 495 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 464 1677
Size: 464 1213
BtsI GCAGTG_nn'
Cuts at: 0 1455 1677
Size: 1455 222
DraI TTT'AAA
Cuts at: 0 65 135 1677
Size: 65 70 1542
Fragments arranged by size:
1542 70 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 471 1209 1677
Size: 471 738 468
Fragments arranged by size:
738 471 468
EarI CTCTTCn'nnn_
Cuts at: 0 296 482 1677
Size: 296 186 1195
Fragments arranged by size:
1195 296 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 140 395 988 1386 1677
Size: 29 111 255 593 398 291
Fragments arranged by size:
593 398 291 255 111 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 921 924 953 955 987 1105 1137
Size: 892 29 3 29 2 32 118 32
Cuts at: 1137 1576 1608 1677
Size: 439 32 69
Fragments arranged by size:
892 439 118 69 32 32 32 29
29 3 2
HindIII A'AGCT_T
Cuts at: 0 236 1677
Size: 236 1441
MfeI C'AATT_G
Cuts at: 0 836 1677
Size: 836 841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 638 1618 1677
Size: 396 242 980 59
Fragments arranged by size:
980 396 242 59
MslI CAynn'nnrTG
Cuts at: 0 653 1480 1572 1632 1677
Size: 653 827 92 60 45
Fragments arranged by size:
827 653 92 60 45
NcoI C'CATG_G
Cuts at: 0 1130 1182 1677
Size: 1130 52 495
Fragments arranged by size:
1130 495 52
NdeI CA'TA_TG
Cuts at: 0 1421 1677
Size: 1421 256
NspI r_CATG'y
Cuts at: 0 1431 1577 1677
Size: 1431 146 100
Fragments arranged by size:
1431 146 100
PciI A'CATG_T
Cuts at: 0 1573 1677
Size: 1573 104
PfoI T'CCnGG_A
Cuts at: 0 45 549 636 1677
Size: 45 504 87 1041
Fragments arranged by size:
1041 504 87 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 1677
Size: 98 32 1547
Fragments arranged by size:
1547 98 32
SfcI C'TryA_G
Cuts at: 0 203 1313 1677
Size: 203 1110 364
Fragments arranged by size:
1110 364 203
SmlI C'TyrA_G
Cuts at: 0 246 1063 1070 1595 1677
Size: 246 817 7 525 82
Fragments arranged by size:
817 525 246 82 7
SpeI A'CTAG_T
Cuts at: 0 799 1677
Size: 799 878
SspI AAT'ATT
Cuts at: 0 1101 1521 1677
Size: 1101 420 156
Fragments arranged by size:
1101 420 156
StyI C'CwwG_G
Cuts at: 0 337 1130 1182 1677
Size: 337 793 52 495
Fragments arranged by size:
793 495 337 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 837 1677
Size: 837 840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 983 1677
Size: 983 694
XmnI GAAnn'nnTTC
Cuts at: 0 224 918 1677
Size: 224 694 759
Fragments arranged by size:
759 694 224
Enzymes that do cut:
AcuI AflII AflIII AleI ApoI BamHI BanII BbsI
BciVI BclI BplI BpmI BpuEI BsaI BsaBI Bsp1286I
BspHI BsrDI BsrFI BstYI BtgI BtgZI BtsI DraI
DrdI EarI Eco57MI Hin4I HindIII MfeI MmeI MslI
NcoI NdeI NspI PciI PfoI PpiI SfcI SmlI
SpeI SspI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI ZraI