Restriction map for
pFN21AE1982
(Linear) (Six-base) MAPSORT of: KIEE1982.seq Check: 3744 from: 1 to: 1674
KIEE1982.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1348 1674
Size: 1226 122 326
Fragments arranged by size:
1226 326 122
AfeI AGC'GCT
Cuts at: 0 23 1674
Size: 23 1651
AleI CACnn'nnGTG
Cuts at: 0 463 758 1674
Size: 463 295 916
Fragments arranged by size:
916 463 295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 676 1674
Size: 358 318 998
Fragments arranged by size:
998 358 318
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 1674
Size: 1237 437
ApaI G_GGCC'C
Cuts at: 0 47 99 411 1674
Size: 47 52 312 1263
Fragments arranged by size:
1263 312 52 47
ApaLI G'TGCA_C
Cuts at: 0 1516 1674
Size: 1516 158
ApoI r'AATT_y
Cuts at: 0 843 1429 1674
Size: 843 586 245
Fragments arranged by size:
843 586 245
AseI AT'TA_AT
Cuts at: 0 746 1674
Size: 746 928
AvaI C'yCGr_G
Cuts at: 0 35 98 272 1674
Size: 35 63 174 1402
Fragments arranged by size:
1402 174 63 35
BanI G'GyrC_C
Cuts at: 0 149 229 1210 1674
Size: 149 80 981 464
Fragments arranged by size:
981 464 149 80
BanII G_rGCy'C
Cuts at: 0 47 61 99 411 1674
Size: 47 14 38 312 1263
Fragments arranged by size:
1263 312 47 38 14
BbeI G_GCGC'C
Cuts at: 0 153 233 1674
Size: 153 80 1441
Fragments arranged by size:
1441 153 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 819 1052 1629 1674
Size: 819 233 577 45
Fragments arranged by size:
819 577 233 45
BciVI GTATCCnnnnn_n'
Cuts at: 0 457 1674
Size: 457 1217
BglI GCCn_nnn'nGGC
Cuts at: 0 76 1674
Size: 76 1598
BglII A'GATC_T
Cuts at: 0 1244 1278 1674
Size: 1244 34 396
Fragments arranged by size:
1244 396 34
Bme1580I G_kGCm'C
Cuts at: 0 47 99 411 737 1520 1674
Size: 47 52 312 326 783 154
Fragments arranged by size:
783 326 312 154 52 47
BmtI G_CTAG'C
Cuts at: 0 628 1674
Size: 628 1046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1214 1273 1316 1674
Size: 653 561 59 43 358
Fragments arranged by size:
653 561 358 59 43
BsaHI Gr'CG_yC
Cuts at: 0 64 150 230 1674
Size: 64 86 80 1444
Fragments arranged by size:
1444 86 80 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 1292 1322 1674
Size: 364 30 898 30 352
Fragments arranged by size:
898 364 352 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 1313 1674
Size: 298 1015 361
Fragments arranged by size:
1015 361 298
BseYI C'CCAG_C
Cuts at: 0 87 92 410 1140 1674
Size: 87 5 318 730 534
Fragments arranged by size:
730 534 318 87 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1674
Size: 536 1138
BsiEI CG_ry'CG
Cuts at: 0 80 177 225 1674
Size: 80 97 48 1449
Fragments arranged by size:
1449 97 80 48
BsiHKAI G_wGCw'C
Cuts at: 0 682 1520 1674
Size: 682 838 154
Fragments arranged by size:
838 682 154
BsmI GAATG_Cn'
Cuts at: 0 1296 1674
Size: 1296 378
Bsp1286I G_dGCh'C
Cuts at: 0 47 61 99 411 682 737 1520 1674
Size: 47 14 38 312 271 55 783 154
Fragments arranged by size:
783 312 271 154 55 47 38 14
BsrBI CCG'CTC
Cuts at: 0 690 1674
Size: 690 984
BsrDI GCAATG_nn'
Cuts at: 0 1199 1674
Size: 1199 475
BsrGI T'GTAC_A
Cuts at: 0 1646 1674
Size: 1646 28
BstAPI GCAn_nnn'nTGC
Cuts at: 0 434 1563 1674
Size: 434 1129 111
Fragments arranged by size:
1129 434 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 1674
Size: 240 1434
BstYI r'GATC_y
Cuts at: 0 1244 1278 1674
Size: 1244 34 396
Fragments arranged by size:
1244 396 34
Bsu36I CC'TnA_GG
Cuts at: 0 265 1674
Size: 265 1409
BtgI C'CryG_G
Cuts at: 0 617 795 819 831 1466 1674
Size: 617 178 24 12 635 208
Fragments arranged by size:
635 617 208 178 24 12
BtsI GCAGTG_nn'
Cuts at: 0 524 1674
Size: 524 1150
EaeI y'GGCC_r
Cuts at: 0 77 140 170 816 1353 1674
Size: 77 63 30 646 537 321
Fragments arranged by size:
646 537 321 77 63 30
EagI C'GGCC_G
Cuts at: 0 77 1674
Size: 77 1597
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 57 1674
Size: 57 1617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1214 1226 1273 1316 1348 1674
Size: 653 561 12 47 43 32 326
Fragments arranged by size:
653 561 326 47 43 32 12
EcoO109I rG'GnC_Cy
Cuts at: 0 261 269 1674
Size: 261 8 1405
Fragments arranged by size:
1405 261 8
EcoRV GAT'ATC
Cuts at: 0 1490 1674
Size: 1490 184
FspI TGC'GCA
Cuts at: 0 1567 1674
Size: 1567 107
FspAI rTGC'GCAy
Cuts at: 0 1567 1674
Size: 1567 107
HaeII r_GCGC'y
Cuts at: 0 25 153 233 1674
Size: 25 128 80 1441
Fragments arranged by size:
1441 128 80 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 1474 1506 1674
Size: 364 32 1078 32 168
Fragments arranged by size:
1078 364 168 32 32
HincII GTy'rAC
Cuts at: 0 1163 1674
Size: 1163 511
HindIII A'AGCT_T
Cuts at: 0 485 857 1674
Size: 485 372 817
Fragments arranged by size:
817 485 372
KasI G'GCGC_C
Cuts at: 0 149 229 1674
Size: 149 80 1445
Fragments arranged by size:
1445 149 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1674
Size: 1196 478
MslI CAynn'nnrTG
Cuts at: 0 463 510 658 758 1573 1674
Size: 463 47 148 100 815 101
Fragments arranged by size:
815 463 148 101 100 47
MspA1I CmG'CkG
Cuts at: 0 49 1144 1609 1674
Size: 49 1095 465 65
Fragments arranged by size:
1095 465 65 49
NarI GG'CG_CC
Cuts at: 0 150 230 1674
Size: 150 80 1444
Fragments arranged by size:
1444 150 80
NcoI C'CATG_G
Cuts at: 0 617 795 831 1674
Size: 617 178 36 843
Fragments arranged by size:
843 617 178 36
NheI G'CTAG_C
Cuts at: 0 624 1674
Size: 624 1050
PasI CC'CwG_GG
Cuts at: 0 54 1674
Size: 54 1620
PflMI CCAn_nnn'nTGG
Cuts at: 0 895 1674
Size: 895 779
PfoI T'CCnGG_A
Cuts at: 0 700 1250 1674
Size: 700 550 424
Fragments arranged by size:
700 550 424
PpuMI rG'GwC_Cy
Cuts at: 0 261 269 1674
Size: 261 8 1405
Fragments arranged by size:
1405 261 8
PspOMI G'GGCC_C
Cuts at: 0 43 95 407 1674
Size: 43 52 312 1267
Fragments arranged by size:
1267 312 52 43
PvuI CG_AT'CG
Cuts at: 0 225 1674
Size: 225 1449
PvuII CAG'CTG
Cuts at: 0 1144 1609 1674
Size: 1144 465 65
Fragments arranged by size:
1144 465 65
SfcI C'TryA_G
Cuts at: 0 156 1674
Size: 156 1518
SfoI GGC'GCC
Cuts at: 0 151 231 1674
Size: 151 80 1443
Fragments arranged by size:
1443 151 80
SmaI CCC'GGG
Cuts at: 0 37 100 274 1674
Size: 37 63 174 1400
Fragments arranged by size:
1400 174 63 37
StyI C'CwwG_G
Cuts at: 0 617 783 795 831 1085 1674
Size: 617 166 12 36 254 589
Fragments arranged by size:
617 589 254 166 36 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1194 1674
Size: 1194 480
TatI w'GTAC_w
Cuts at: 0 1646 1674
Size: 1646 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1479 1511 1674
Size: 1479 32 163
Fragments arranged by size:
1479 163 32
XmaI C'CCGG_G
Cuts at: 0 35 98 272 1674
Size: 35 63 174 1402
Fragments arranged by size:
1402 174 63 35
XmnI GAAnn'nnTTC
Cuts at: 0 1636 1674
Size: 1636 38
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AvaI BanI BanII BbeI BbsI BciVI BglI
BglII Bme1580I BmtI BpmI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI
EciI Eco57MI EcoO109I EcoRV FspI FspAI HaeII Hin4I
HincII HindIII KasI MmeI MslI MspA1I NarI NcoI
NheI PasI PflMI PfoI PpuMI PspOMI PvuI PvuII
SfcI SfoI SmaI StyI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI AscI AsiSI AvrII BaeI BamHI BbvCI
BcgI BclI BfrBI BlpI BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI
BspHI BspMI BsrFI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI
EcoNI EcoRI FalI FseI HpaI KpnI MfeI MluI
MscI NaeI NdeI NgoMIV NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI ZraI