Restriction map for
pFN21AE1966
(Linear) (Six-base) MAPSORT of: KIEE1966.seq Check: 6985 from: 1 to: 1572
KIEE1966.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1451 1572
Size: 1451 121
AccI GT'mk_AC
Cuts at: 0 1566 1572
Size: 1566 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1054 1193 1223 1572
Size: 806 248 139 30 349
Fragments arranged by size:
806 349 248 139 30
AflIII A'CryG_T
Cuts at: 0 1271 1572
Size: 1271 301
AlwNI CAG_nnn'CTG
Cuts at: 0 1554 1572
Size: 1554 18
ApoI r'AATT_y
Cuts at: 0 307 488 564 1383 1572
Size: 307 181 76 819 189
Fragments arranged by size:
819 307 189 181 76
BanII G_rGCy'C
Cuts at: 0 409 766 1572
Size: 409 357 806
Fragments arranged by size:
806 409 357
BbsI GAAGACnn'nnnn_
Cuts at: 0 456 960 1060 1572
Size: 456 504 100 512
Fragments arranged by size:
512 504 456 100
BciVI GTATCCnnnnn_n'
Cuts at: 0 844 1302 1572
Size: 844 458 270
Fragments arranged by size:
844 458 270
BfrBI ATG'CAT
Cuts at: 0 1450 1572
Size: 1450 122
BglI GCCn_nnn'nGGC
Cuts at: 0 916 1572
Size: 916 656
Bme1580I G_kGCm'C
Cuts at: 0 1417 1572
Size: 1417 155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 534 1572
Size: 534 1038
Bpu10I CC'TnA_GC
Cuts at: 0 1391 1572
Size: 1391 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 234 1572
Size: 52 182 1338
Fragments arranged by size:
1338 182 52
BsaWI w'CCGG_w
Cuts at: 0 298 1572
Size: 298 1274
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1370 1572
Size: 1370 202
BseYI C'CCAG_C
Cuts at: 0 1193 1572
Size: 1193 379
Bsp1286I G_dGCh'C
Cuts at: 0 409 766 1417 1572
Size: 409 357 651 155
Fragments arranged by size:
651 409 357 155
BspEI T'CCGG_A
Cuts at: 0 298 1572
Size: 298 1274
BspHI T'CATG_A
Cuts at: 0 132 1572
Size: 132 1440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1451 1572
Size: 1451 121
BsrBI CCG'CTC
Cuts at: 0 248 1572
Size: 248 1324
BsrDI GCAATG_nn'
Cuts at: 0 32 716 1404 1450 1572
Size: 32 684 688 46 122
Fragments arranged by size:
688 684 122 46 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1087 1162 1435 1572
Size: 1087 75 273 137
Fragments arranged by size:
1087 273 137 75
BstYI r'GATC_y
Cuts at: 0 803 1572
Size: 803 769
Bsu36I CC'TnA_GG
Cuts at: 0 440 1572
Size: 440 1132
BtsI GCAGTG_nn'
Cuts at: 0 75 1572
Size: 75 1497
EaeI y'GGCC_r
Cuts at: 0 78 642 1572
Size: 78 564 930
Fragments arranged by size:
930 564 78
EarI CTCTTCn'nnn_
Cuts at: 0 503 1508 1572
Size: 503 1005 64
Fragments arranged by size:
1005 503 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1103 1572
Size: 1103 469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1054 1193 1223 1572
Size: 806 248 139 30 349
Fragments arranged by size:
806 349 248 139 30
EcoRI G'AATT_C
Cuts at: 0 488 1572
Size: 488 1084
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 46 811 1572
Size: 46 765 761
Fragments arranged by size:
765 761 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 598 630 1572
Size: 108 32 458 32 942
Fragments arranged by size:
942 458 108 32 32
HincII GTy'rAC
Cuts at: 0 1377 1572
Size: 1377 195
HindIII A'AGCT_T
Cuts at: 0 1205 1572
Size: 1205 367
MfeI C'AATT_G
Cuts at: 0 1421 1572
Size: 1421 151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1281 1284 1531 1572
Size: 1281 3 247 41
Fragments arranged by size:
1281 247 41 3
MscI TGG'CCA
Cuts at: 0 644 1572
Size: 644 928
MslI CAynn'nnrTG
Cuts at: 0 1370 1572
Size: 1370 202
MspA1I CmG'CkG
Cuts at: 0 477 840 1225 1572
Size: 477 363 385 347
Fragments arranged by size:
477 385 363 347
NdeI CA'TA_TG
Cuts at: 0 1494 1572
Size: 1494 78
NsiI A_TGCA'T
Cuts at: 0 1452 1572
Size: 1452 120
NspI r_CATG'y
Cuts at: 0 1275 1572
Size: 1275 297
PciI A'CATG_T
Cuts at: 0 1271 1572
Size: 1271 301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 990 1022 1572
Size: 990 32 550
Fragments arranged by size:
990 550 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 518 550 1572
Size: 518 32 1022
Fragments arranged by size:
1022 518 32
PstI C_TGCA'G
Cuts at: 0 708 1224 1431 1572
Size: 708 516 207 141
Fragments arranged by size:
708 516 207 141
PvuII CAG'CTG
Cuts at: 0 477 840 1225 1572
Size: 477 363 385 347
Fragments arranged by size:
477 385 363 347
SfcI C'TryA_G
Cuts at: 0 282 521 704 1135 1220 1427 1572
Size: 282 239 183 431 85 207 145
Fragments arranged by size:
431 282 239 207 183 145 85
SmlI C'TyrA_G
Cuts at: 0 31 249 1572
Size: 31 218 1323
Fragments arranged by size:
1323 218 31
StyI C'CwwG_G
Cuts at: 0 496 869 1572
Size: 496 373 703
Fragments arranged by size:
703 496 373
TatI w'GTAC_w
Cuts at: 0 672 1572
Size: 672 900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1241 1273 1572
Size: 1241 32 299
Fragments arranged by size:
1241 299 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 918 1572
Size: 918 654
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI BanII BbsI
BciVI BfrBI BglI Bme1580I BplI Bpu10I BpuEI BsaWI
BseRI BseYI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BstAPI BstYI Bsu36I BtsI EaeI EarI EciI Eco57MI
EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NdeI NsiI NspI PciI PpiI PsrI
PstI PvuII SfcI SmlI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BglII BlpI BmgBI BmrI BmtI BpmI BsaI BsaAI
BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI