Restriction map for
pFN21AE1961
(Linear) (Six-base) MAPSORT of: KIEE1961.seq Check: 4309 from: 1 to: 1572
KIEE1961.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 747 1572
Size: 747 825
AccI GT'mk_AC
Cuts at: 0 932 1572
Size: 932 640
Acc65I G'GTAC_C
Cuts at: 0 1328 1572
Size: 1328 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1446 1529 1572
Size: 1446 83 43
Fragments arranged by size:
1446 83 43
AflII C'TTAA_G
Cuts at: 0 1447 1572
Size: 1447 125
AlwNI CAG_nnn'CTG
Cuts at: 0 1278 1572
Size: 1278 294
ApaI G_GGCC'C
Cuts at: 0 1345 1572
Size: 1345 227
ApoI r'AATT_y
Cuts at: 0 895 1487 1537 1572
Size: 895 592 50 35
Fragments arranged by size:
895 592 50 35
AvaI C'yCGr_G
Cuts at: 0 632 974 1572
Size: 632 342 598
Fragments arranged by size:
632 598 342
AvrII C'CTAG_G
Cuts at: 0 1168 1572
Size: 1168 404
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 795 828 1320 1353 1572
Size: 795 33 492 33 219
Fragments arranged by size:
795 492 219 33 33
BanI G'GyrC_C
Cuts at: 0 241 1328 1572
Size: 241 1087 244
Fragments arranged by size:
1087 244 241
BanII G_rGCy'C
Cuts at: 0 164 455 728 1212 1345 1572
Size: 164 291 273 484 133 227
Fragments arranged by size:
484 291 273 227 164 133
BbvCI CC'TCA_GC
Cuts at: 0 1130 1572
Size: 1130 442
BciVI GTATCCnnnnn_n'
Cuts at: 0 823 1572
Size: 823 749
BglI GCCn_nnn'nGGC
Cuts at: 0 1183 1572
Size: 1183 389
Bme1580I G_kGCm'C
Cuts at: 0 507 561 1345 1572
Size: 507 54 784 227
Fragments arranged by size:
784 507 227 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 944 1572
Size: 222 722 628
Fragments arranged by size:
722 628 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 445 742 1108 1198 1572
Size: 176 269 297 366 90 374
Fragments arranged by size:
374 366 297 269 176 90
Bpu10I CC'TnA_GC
Cuts at: 0 727 1130 1572
Size: 727 403 442
Fragments arranged by size:
727 442 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1572
Size: 996 576
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 1572
Size: 214 1358
BsaWI w'CCGG_w
Cuts at: 0 638 1572
Size: 638 934
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 679 1147 1572
Size: 469 210 468 425
Fragments arranged by size:
469 468 425 210
BsiHKAI G_wGCw'C
Cuts at: 0 254 728 1572
Size: 254 474 844
Fragments arranged by size:
844 474 254
BsmI GAATG_Cn'
Cuts at: 0 469 1572
Size: 469 1103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1126 1572
Size: 1126 446
Bsp1286I G_dGCh'C
Cuts at: 0 164 254 455 507 561 728 1212 1345
Size: 164 90 201 52 54 167 484 133
Cuts at: 1345 1572
Size: 227
Fragments arranged by size:
484 227 201 167 164 133 90 54
52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 747 1232 1572
Size: 747 485 340
Fragments arranged by size:
747 485 340
BsrBI CCG'CTC
Cuts at: 0 767 1572
Size: 767 805
BsrDI GCAATG_nn'
Cuts at: 0 272 1572
Size: 272 1300
BstBI TT'CG_AA
Cuts at: 0 49 1572
Size: 49 1523
BstXI CCAn_nnnn'nTGG
Cuts at: 0 915 1572
Size: 915 657
BtgI C'CryG_G
Cuts at: 0 227 464 538 1199 1572
Size: 227 237 74 661 373
Fragments arranged by size:
661 373 237 227 74
BtsI GCAGTG_nn'
Cuts at: 0 1097 1572
Size: 1097 475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1064 1099 1572
Size: 1064 35 473
Fragments arranged by size:
1064 473 35
EaeI y'GGCC_r
Cuts at: 0 230 707 917 966 1202 1572
Size: 230 477 210 49 236 370
Fragments arranged by size:
477 370 236 230 210 49
EarI CTCTTCn'nnn_
Cuts at: 0 697 1031 1091 1505 1572
Size: 697 334 60 414 67
Fragments arranged by size:
697 414 334 67 60
EcoICRI GAG'CTC
Cuts at: 0 726 1572
Size: 726 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 445 742 1108 1198 1446 1529 1572
Size: 176 269 297 366 90 248 83 43
Fragments arranged by size:
366 297 269 248 176 90 83 43
EcoO109I rG'GnC_Cy
Cuts at: 0 497 1342 1572
Size: 497 845 230
Fragments arranged by size:
845 497 230
FspI TGC'GCA
Cuts at: 0 1228 1572
Size: 1228 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 766 798 1513 1545 1572
Size: 27 739 32 715 32 27
Fragments arranged by size:
739 715 32 32 27 27
KpnI G_GTAC'C
Cuts at: 0 1332 1572
Size: 1332 240
MscI TGG'CCA
Cuts at: 0 709 968 1204 1572
Size: 709 259 236 368
Fragments arranged by size:
709 368 259 236
MspA1I CmG'CkG
Cuts at: 0 735 994 1370 1572
Size: 735 259 376 202
Fragments arranged by size:
735 376 259 202
NcoI C'CATG_G
Cuts at: 0 464 1199 1572
Size: 464 735 373
Fragments arranged by size:
735 464 373
NdeI CA'TA_TG
Cuts at: 0 914 1572
Size: 914 658
NspI r_CATG'y
Cuts at: 0 537 1572
Size: 537 1035
PasI CC'CwG_GG
Cuts at: 0 1150 1572
Size: 1150 422
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 1257 1572
Size: 705 552 315
Fragments arranged by size:
705 552 315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1316 1348 1572
Size: 1316 32 224
Fragments arranged by size:
1316 224 32
PpuMI rG'GwC_Cy
Cuts at: 0 497 1572
Size: 497 1075
PshAI GACnn'nnGTC
Cuts at: 0 93 1572
Size: 93 1479
PspOMI G'GGCC_C
Cuts at: 0 1341 1572
Size: 1341 231
PstI C_TGCA'G
Cuts at: 0 740 758 993 1274 1572
Size: 740 18 235 281 298
Fragments arranged by size:
740 298 281 235 18
PvuII CAG'CTG
Cuts at: 0 735 994 1572
Size: 735 259 578
Fragments arranged by size:
735 578 259
SacI G_AGCT'C
Cuts at: 0 728 1572
Size: 728 844
SfcI C'TryA_G
Cuts at: 0 736 754 792 989 1270 1572
Size: 736 18 38 197 281 302
Fragments arranged by size:
736 302 281 197 38 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1183 1572
Size: 1183 389
SmaI CCC'GGG
Cuts at: 0 976 1572
Size: 976 596
SmlI C'TyrA_G
Cuts at: 0 1011 1447 1572
Size: 1011 436 125
Fragments arranged by size:
1011 436 125
StuI AGG'CCT
Cuts at: 0 1102 1315 1572
Size: 1102 213 257
Fragments arranged by size:
1102 257 213
StyI C'CwwG_G
Cuts at: 0 464 1168 1199 1310 1572
Size: 464 704 31 111 262
Fragments arranged by size:
704 464 262 111 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 497 529 1572
Size: 244 32 221 32 1043
Fragments arranged by size:
1043 244 221 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 1572
Size: 717 855
XmaI C'CCGG_G
Cuts at: 0 974 1572
Size: 974 598
XmnI GAAnn'nnTTC
Cuts at: 0 177 1572
Size: 177 1395
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AlwNI ApaI ApoI
AvaI AvrII BaeI BanI BanII BbvCI BciVI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI BseRI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstBI
BstXI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoO109I FspI Hin4I KpnI MscI MspA1I NcoI NdeI
NspI PasI PflMI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SfcI SfiI SmaI SmlI StuI StyI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI BamHI BbeI BbsI
BcgI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MmeI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XhoI ZraI