Restriction map for
pFN21AE1957
(Linear) (Six-base) MAPSORT of: KIEE1957.seq Check: 562 from: 1 to: 1536
KIEE1957.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 754 1536
Size: 754 782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 635 1027 1269 1536
Size: 394 241 392 242 267
Fragments arranged by size:
394 392 267 242 241
AflIII A'CryG_T
Cuts at: 0 791 1073 1536
Size: 791 282 463
Fragments arranged by size:
791 463 282
AlwNI CAG_nnn'CTG
Cuts at: 0 333 384 1005 1179 1536
Size: 333 51 621 174 357
Fragments arranged by size:
621 357 333 174 51
ApoI r'AATT_y
Cuts at: 0 429 1536
Size: 429 1107
AvaI C'yCGr_G
Cuts at: 0 595 1536
Size: 595 941
BanI G'GyrC_C
Cuts at: 0 196 292 481 1536
Size: 196 96 189 1055
Fragments arranged by size:
1055 196 189 96
BanII G_rGCy'C
Cuts at: 0 210 226 1330 1536
Size: 210 16 1104 206
Fragments arranged by size:
1104 210 206 16
BbeI G_GCGC'C
Cuts at: 0 200 1536
Size: 200 1336
BbsI GAAGACnn'nnnn_
Cuts at: 0 1275 1536
Size: 1275 261
BbvCI CC'TCA_GC
Cuts at: 0 609 1536
Size: 609 927
BfrBI ATG'CAT
Cuts at: 0 883 1536
Size: 883 653
BglI GCCn_nnn'nGGC
Cuts at: 0 653 1536
Size: 653 883
BlpI GC'TnA_GC
Cuts at: 0 109 1536
Size: 109 1427
Bme1580I G_kGCm'C
Cuts at: 0 486 1536
Size: 486 1050
BmrI ACTGGGnnnn_n'
Cuts at: 0 480 1195 1518 1536
Size: 480 715 323 18
Fragments arranged by size:
715 480 323 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 490 1536
Size: 490 1046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1201 1254 1438 1473 1536
Size: 568 633 53 184 35 63
Fragments arranged by size:
633 568 184 63 53 35
Bpu10I CC'TnA_GC
Cuts at: 0 609 1536
Size: 609 927
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 1536
Size: 178 1358
BsaHI Gr'CG_yC
Cuts at: 0 129 197 1536
Size: 129 68 1339
Fragments arranged by size:
1339 129 68
BsaWI w'CCGG_w
Cuts at: 0 5 1536
Size: 5 1531
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1174 1204 1327 1357 1536
Size: 1174 30 123 30 179
Fragments arranged by size:
1174 179 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 856 1228 1324 1536
Size: 31 825 372 96 212
Fragments arranged by size:
825 372 212 96 31
BseYI C'CCAG_C
Cuts at: 0 219 229 256 714 1536
Size: 219 10 27 458 822
Fragments arranged by size:
822 458 219 27 10
BsiEI CG_ry'CG
Cuts at: 0 60 1536
Size: 60 1476
BsiHKAI G_wGCw'C
Cuts at: 0 1366 1536
Size: 1366 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 461 510 1536
Size: 461 49 1026
Fragments arranged by size:
1026 461 49
Bsp1286I G_dGCh'C
Cuts at: 0 210 226 486 1330 1366 1536
Size: 210 16 260 844 36 170
Fragments arranged by size:
844 260 210 170 36 16
BspEI T'CCGG_A
Cuts at: 0 5 1536
Size: 5 1531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 380 431 754 1070 1280 1536
Size: 380 51 323 316 210 256
Fragments arranged by size:
380 323 316 256 210 51
BsrFI r'CCGG_y
Cuts at: 0 113 1163 1536
Size: 113 1050 373
Fragments arranged by size:
1050 373 113
BsrGI T'GTAC_A
Cuts at: 0 794 1536
Size: 794 742
BssHII G'CGCG_C
Cuts at: 0 104 323 1536
Size: 104 219 1213
Fragments arranged by size:
1213 219 104
BssSI C'ACGA_G
Cuts at: 0 352 475 1384 1536
Size: 352 123 909 152
Fragments arranged by size:
909 352 152 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1370 1536
Size: 1370 166
BstEII G'GTnAC_C
Cuts at: 0 1422 1536
Size: 1422 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 306 1536
Size: 306 1230
BstYI r'GATC_y
Cuts at: 0 1120 1536
Size: 1120 416
Bsu36I CC'TnA_GG
Cuts at: 0 1172 1536
Size: 1172 364
BtgI C'CryG_G
Cuts at: 0 54 83 438 757 1040 1331 1536
Size: 54 29 355 319 283 291 205
Fragments arranged by size:
355 319 291 283 205 54 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 16 1536
Size: 16 1520
BtsI GCAGTG_nn'
Cuts at: 0 1060 1536
Size: 1060 476
DraIII CAC_nnn'GTG
Cuts at: 0 1386 1526 1536
Size: 1386 140 10
Fragments arranged by size:
1386 140 10
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 1536
Size: 15 1521
EaeI y'GGCC_r
Cuts at: 0 57 360 1161 1536
Size: 57 303 801 375
Fragments arranged by size:
801 375 303 57
EagI C'GGCC_G
Cuts at: 0 57 1536
Size: 57 1479
EarI CTCTTCn'nnn_
Cuts at: 0 241 1238 1536
Size: 241 997 298
Fragments arranged by size:
997 298 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 568 635 1027 1201 1254 1269 1438
Size: 394 174 67 392 174 53 15 169
Cuts at: 1438 1473 1536
Size: 35 63
Fragments arranged by size:
394 392 174 174 169 67 63 53
35 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 1210 1536
Size: 376 834 326
Fragments arranged by size:
834 376 326
EcoO109I rG'GnC_Cy
Cuts at: 0 330 381 906 1446 1457 1536
Size: 330 51 525 540 11 79
Fragments arranged by size:
540 525 330 79 51 11
EcoRV GAT'ATC
Cuts at: 0 993 1536
Size: 993 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1276 1536
Size: 1276 260
FspI TGC'GCA
Cuts at: 0 700 1536
Size: 700 836
HaeII r_GCGC'y
Cuts at: 0 200 263 1536
Size: 200 63 1273
Fragments arranged by size:
1273 200 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1536
Size: 1174 32 330
Fragments arranged by size:
1174 330 32
HincII GTy'rAC
Cuts at: 0 420 1536
Size: 420 1116
HindIII A'AGCT_T
Cuts at: 0 819 1536
Size: 819 717
HpaI GTT'AAC
Cuts at: 0 420 1536
Size: 420 1116
KasI G'GCGC_C
Cuts at: 0 196 1536
Size: 196 1340
MslI CAynn'nnrTG
Cuts at: 0 655 1024 1045 1536
Size: 655 369 21 491
Fragments arranged by size:
655 491 369 21
MspA1I CmG'CkG
Cuts at: 0 56 613 809 949 1536
Size: 56 557 196 140 587
Fragments arranged by size:
587 557 196 140 56
NaeI GCC'GGC
Cuts at: 0 115 1536
Size: 115 1421
NarI GG'CG_CC
Cuts at: 0 197 1536
Size: 197 1339
NcoI C'CATG_G
Cuts at: 0 438 757 1040 1536
Size: 438 319 283 496
Fragments arranged by size:
496 438 319 283
NdeI CA'TA_TG
Cuts at: 0 1350 1536
Size: 1350 186
NgoMIV G'CCGG_C
Cuts at: 0 113 1536
Size: 113 1423
NsiI A_TGCA'T
Cuts at: 0 885 1536
Size: 885 651
NspI r_CATG'y
Cuts at: 0 78 576 795 1536
Size: 78 498 219 741
Fragments arranged by size:
741 498 219 78
PciI A'CATG_T
Cuts at: 0 791 1536
Size: 791 745
PfoI T'CCnGG_A
Cuts at: 0 908 1536
Size: 908 628
PpuMI rG'GwC_Cy
Cuts at: 0 381 906 1446 1457 1536
Size: 381 525 540 11 79
Fragments arranged by size:
540 525 381 79 11
PspXI vC'TCGA_Gb
Cuts at: 0 595 1536
Size: 595 941
PstI C_TGCA'G
Cuts at: 0 751 1001 1055 1536
Size: 751 250 54 481
Fragments arranged by size:
751 481 250 54
PvuII CAG'CTG
Cuts at: 0 613 949 1536
Size: 613 336 587
Fragments arranged by size:
613 587 336
SacII CC_GC'GG
Cuts at: 0 57 1536
Size: 57 1479
SanDI GG'GwC_CC
Cuts at: 0 1446 1536
Size: 1446 90
SapI GCTCTTCn'nnn_
Cuts at: 0 1238 1536
Size: 1238 298
SfcI C'TryA_G
Cuts at: 0 747 997 1051 1536
Size: 747 250 54 485
Fragments arranged by size:
747 485 250 54
SfoI GGC'GCC
Cuts at: 0 198 1536
Size: 198 1338
SmlI C'TyrA_G
Cuts at: 0 595 1536
Size: 595 941
StuI AGG'CCT
Cuts at: 0 278 1490 1536
Size: 278 1212 46
Fragments arranged by size:
1212 278 46
StyI C'CwwG_G
Cuts at: 0 438 757 1040 1536
Size: 438 319 283 496
Fragments arranged by size:
496 438 319 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 469 1536
Size: 469 1067
TatI w'GTAC_w
Cuts at: 0 751 794 1536
Size: 751 43 742
Fragments arranged by size:
751 742 43
Tth111I GACn'n_nGTC
Cuts at: 0 1035 1536
Size: 1035 501
XhoI C'TCGA_G
Cuts at: 0 595 1536
Size: 595 941
XmnI GAAnn'nnTTC
Cuts at: 0 1247 1536
Size: 1247 289
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BfrBI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PfoI PpuMI
PspXI PstI PvuII SacII SanDI SapI SfcI SfoI
SmlI StuI StyI TaqII TatI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BglII BmgBI
BmtI BpuEI BsaAI BsaBI BsgI BsiWI BsmI BspHI
BsrBI BsrDI BstBI BstZ17I ClaI CspCI DraI EciI
EcoICRI EcoRI FseI FspAI KpnI MfeI MluI MmeI
MscI NheI NotI NruI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII
SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XmaI ZraI