Restriction map for
pFN21AE1954
(Linear) (Six-base) MAPSORT of: KIEE1954.seq Check: 5641 from: 1 to: 1530
KIEE1954.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 404 1262 1530
Size: 332 72 858 268
Fragments arranged by size:
858 332 268 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 608 1117 1530
Size: 608 509 413
Fragments arranged by size:
608 509 413
ApoI r'AATT_y
Cuts at: 0 141 739 931 1530
Size: 141 598 192 599
Fragments arranged by size:
599 598 192 141
BanI G'GyrC_C
Cuts at: 0 183 205 1530
Size: 183 22 1325
Fragments arranged by size:
1325 183 22
BanII G_rGCy'C
Cuts at: 0 51 559 815 971 1530
Size: 51 508 256 156 559
Fragments arranged by size:
559 508 256 156 51
BbeI G_GCGC'C
Cuts at: 0 209 1530
Size: 209 1321
BclI T'GATC_A
Cuts at: 0 399 1530
Size: 399 1131
BglII A'GATC_T
Cuts at: 0 220 1530
Size: 220 1310
Bme1580I G_kGCm'C
Cuts at: 0 101 1530
Size: 101 1429
BmrI ACTGGGnnnn_n'
Cuts at: 0 265 1530
Size: 265 1265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 842 1530
Size: 35 807 688
Fragments arranged by size:
807 688 35
Bpu10I CC'TnA_GC
Cuts at: 0 63 1530
Size: 63 1467
BsaHI Gr'CG_yC
Cuts at: 0 206 1530
Size: 206 1324
BsaWI w'CCGG_w
Cuts at: 0 149 1530
Size: 149 1381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2 32 1530
Size: 2 30 1498
Fragments arranged by size:
1498 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 1530
Size: 10 1520
BseYI C'CCAG_C
Cuts at: 0 1266 1518 1530
Size: 1266 252 12
Fragments arranged by size:
1266 252 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1530
Size: 878 652
BsiEI CG_ry'CG
Cuts at: 0 95 308 1530
Size: 95 213 1222
Fragments arranged by size:
1222 213 95
BsiHKAI G_wGCw'C
Cuts at: 0 12 559 815 971 1530
Size: 12 547 256 156 559
Fragments arranged by size:
559 547 256 156 12
Bsp1286I G_dGCh'C
Cuts at: 0 12 51 101 559 815 971 1530
Size: 12 39 50 458 256 156 559
Fragments arranged by size:
559 458 256 156 50 39 12
BspEI T'CCGG_A
Cuts at: 0 149 1530
Size: 149 1381
BspHI T'CATG_A
Cuts at: 0 609 951 1530
Size: 609 342 579
Fragments arranged by size:
609 579 342
BsrBI CCG'CTC
Cuts at: 0 304 1530
Size: 304 1226
BsrDI GCAATG_nn'
Cuts at: 0 587 779 1142 1203 1530
Size: 587 192 363 61 327
Fragments arranged by size:
587 363 327 192 61
BssHII G'CGCG_C
Cuts at: 0 25 1530
Size: 25 1505
BssSI C'ACGA_G
Cuts at: 0 40 412 1530
Size: 40 372 1118
Fragments arranged by size:
1118 372 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1411 1530
Size: 1411 119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 1266 1530
Size: 328 938 264
Fragments arranged by size:
938 328 264
BstYI r'GATC_y
Cuts at: 0 220 1530
Size: 220 1310
Bsu36I CC'TnA_GG
Cuts at: 0 1508 1530
Size: 1508 22
BtgI C'CryG_G
Cuts at: 0 1142 1530
Size: 1142 388
BtsI GCAGTG_nn'
Cuts at: 0 827 917 1530
Size: 827 90 613
Fragments arranged by size:
827 613 90
DraI TTT'AAA
Cuts at: 0 848 1530
Size: 848 682
EaeI y'GGCC_r
Cuts at: 0 92 305 1530
Size: 92 213 1225
Fragments arranged by size:
1225 213 92
EagI C'GGCC_G
Cuts at: 0 92 305 1530
Size: 92 213 1225
Fragments arranged by size:
1225 213 92
EcoICRI GAG'CTC
Cuts at: 0 557 813 969 1530
Size: 557 256 156 561
Fragments arranged by size:
561 557 256 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 332 404 842 1262 1530
Size: 35 297 72 438 420 268
Fragments arranged by size:
438 420 297 268 72 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1049 1385 1530
Size: 1049 336 145
Fragments arranged by size:
1049 336 145
HaeII r_GCGC'y
Cuts at: 0 209 1530
Size: 209 1321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 1052 1084 1530
Size: 646 32 374 32 446
Fragments arranged by size:
646 446 374 32 32
HindIII A'AGCT_T
Cuts at: 0 281 1530
Size: 281 1249
KasI G'GCGC_C
Cuts at: 0 205 1530
Size: 205 1325
MfeI C'AATT_G
Cuts at: 0 482 1530
Size: 482 1048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1530
Size: 1167 363
MslI CAynn'nnrTG
Cuts at: 0 580 1430 1530
Size: 580 850 100
Fragments arranged by size:
850 580 100
MspA1I CmG'CkG
Cuts at: 0 310 820 1530
Size: 310 510 710
Fragments arranged by size:
710 510 310
NarI GG'CG_CC
Cuts at: 0 206 1530
Size: 206 1324
NcoI C'CATG_G
Cuts at: 0 1142 1530
Size: 1142 388
NotI GC'GGCC_GC
Cuts at: 0 305 1530
Size: 305 1225
NspI r_CATG'y
Cuts at: 0 264 1518 1530
Size: 264 1254 12
Fragments arranged by size:
1254 264 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 1161 1530
Size: 1161 369
PstI C_TGCA'G
Cuts at: 0 1000 1530
Size: 1000 530
PvuII CAG'CTG
Cuts at: 0 820 1530
Size: 820 710
SacI G_AGCT'C
Cuts at: 0 559 815 971 1530
Size: 559 256 156 559
Fragments arranged by size:
559 559 256 156
ScaI AGT'ACT
Cuts at: 0 617 1530
Size: 617 913
SfcI C'TryA_G
Cuts at: 0 631 996 1530
Size: 631 365 534
Fragments arranged by size:
631 534 365
SfoI GGC'GCC
Cuts at: 0 207 1530
Size: 207 1323
SphI G_CATG'C
Cuts at: 0 264 1530
Size: 264 1266
StyI C'CwwG_G
Cuts at: 0 1142 1530
Size: 1142 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 1037 1530
Size: 73 964 493
Fragments arranged by size:
964 493 73
TatI w'GTAC_w
Cuts at: 0 615 1530
Size: 615 915
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1530
Size: 2 32 1496
Fragments arranged by size:
1496 32 2
XmnI GAAnn'nnTTC
Cuts at: 0 1219 1530
Size: 1219 311
Enzymes that do cut:
AcuI AlfI ApoI BanI BanII BbeI BclI BglII
Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI DraI EaeI EagI EcoICRI Eco57MI EcoNI HaeII
Hin4I HindIII KasI MfeI MmeI MslI MspA1I NarI
NcoI NotI NspI PflMI PstI PvuII SacI ScaI
SfcI SfoI SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspMI
BsrFI BsrGI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraIII DrdI EarI EciI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MluI MscI
NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI