Restriction map for
pFN21AE1953
(Linear) (Six-base) MAPSORT of: KIEE1953.seq Check: 2255 from: 1 to: 1527
KIEE1953.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AccI GT'mk_AC
Cuts at: 0 405 856 1527
Size: 405 451 671
Fragments arranged by size:
671 451 405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 593 1505 1527
Size: 575 18 912 22
Fragments arranged by size:
912 575 22 18
AflIII A'CryG_T
Cuts at: 0 372 1527
Size: 372 1155
AlwNI CAG_nnn'CTG
Cuts at: 0 1113 1527
Size: 1113 414
ApaI G_GGCC'C
Cuts at: 0 932 1527
Size: 932 595
ApaLI G'TGCA_C
Cuts at: 0 1144 1527
Size: 1144 383
ApoI r'AATT_y
Cuts at: 0 744 817 1527
Size: 744 73 710
Fragments arranged by size:
744 710 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 855 888 1527
Size: 855 33 639
Fragments arranged by size:
855 639 33
BamHI G'GATC_C
Cuts at: 0 420 1527
Size: 420 1107
BanII G_rGCy'C
Cuts at: 0 932 1527
Size: 932 595
BbsI GAAGACnn'nnnn_
Cuts at: 0 1044 1527
Size: 1044 483
BbvCI CC'TCA_GC
Cuts at: 0 702 1527
Size: 702 825
BglII A'GATC_T
Cuts at: 0 500 1527
Size: 500 1027
Bme1580I G_kGCm'C
Cuts at: 0 932 1148 1527
Size: 932 216 379
Fragments arranged by size:
932 379 216
BmgBI CAC'GTC
Cuts at: 0 373 1527
Size: 373 1154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1325 1348 1527
Size: 788 537 23 179
Fragments arranged by size:
788 537 179 23
Bpu10I CC'TnA_GC
Cuts at: 0 702 1527
Size: 702 825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1527
Size: 576 951
BsaBI GATnn'nnATC
Cuts at: 0 983 1527
Size: 983 544
BsaWI w'CCGG_w
Cuts at: 0 387 1527
Size: 387 1140
BseYI C'CCAG_C
Cuts at: 0 919 1113 1527
Size: 919 194 414
Fragments arranged by size:
919 414 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1527
Size: 945 582
BsiHKAI G_wGCw'C
Cuts at: 0 1148 1527
Size: 1148 379
Bsp1286I G_dGCh'C
Cuts at: 0 932 1148 1527
Size: 932 216 379
Fragments arranged by size:
932 379 216
BspEI T'CCGG_A
Cuts at: 0 387 1527
Size: 387 1140
BspHI T'CATG_A
Cuts at: 0 441 1154 1449 1527
Size: 441 713 295 78
Fragments arranged by size:
713 441 295 78
BsrDI GCAATG_nn'
Cuts at: 0 349 1527
Size: 349 1178
BsrGI T'GTAC_A
Cuts at: 0 1010 1527
Size: 1010 517
BstXI CCAn_nnnn'nTGG
Cuts at: 0 101 1527
Size: 101 1426
BstYI r'GATC_y
Cuts at: 0 11 384 420 500 1455 1527
Size: 11 373 36 80 955 72
Fragments arranged by size:
955 373 80 72 36 11
BtsI GCAGTG_nn'
Cuts at: 0 1233 1527
Size: 1233 294
DraI TTT'AAA
Cuts at: 0 270 1527
Size: 270 1257
EaeI y'GGCC_r
Cuts at: 0 666 1527
Size: 666 861
EarI CTCTTCn'nnn_
Cuts at: 0 1091 1527
Size: 1091 436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 593 788 1325 1348 1505 1527
Size: 575 18 195 537 23 157 22
Fragments arranged by size:
575 537 195 157 23 22 18
EcoO109I rG'GnC_Cy
Cuts at: 0 929 1527
Size: 929 598
HaeII r_GCGC'y
Cuts at: 0 360 1527
Size: 360 1167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 1422 1454 1527
Size: 369 32 1021 32 73
Fragments arranged by size:
1021 369 73 32 32
HincII GTy'rAC
Cuts at: 0 282 1527
Size: 282 1245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1527
Size: 398 1129
MscI TGG'CCA
Cuts at: 0 668 1527
Size: 668 859
MslI CAynn'nnrTG
Cuts at: 0 289 1527
Size: 289 1238
MspA1I CmG'CkG
Cuts at: 0 645 1110 1527
Size: 645 465 417
Fragments arranged by size:
645 465 417
NdeI CA'TA_TG
Cuts at: 0 528 1527
Size: 528 999
NspI r_CATG'y
Cuts at: 0 1337 1527
Size: 1337 190
PfoI T'CCnGG_A
Cuts at: 0 690 1527
Size: 690 837
PspOMI G'GGCC_C
Cuts at: 0 928 1527
Size: 928 599
PvuII CAG'CTG
Cuts at: 0 645 1110 1527
Size: 645 465 417
Fragments arranged by size:
645 465 417
SexAI A'CCwGG_T
Cuts at: 0 135 1349 1527
Size: 135 1214 178
Fragments arranged by size:
1214 178 135
SfcI C'TryA_G
Cuts at: 0 857 1175 1527
Size: 857 318 352
Fragments arranged by size:
857 352 318
SmlI C'TyrA_G
Cuts at: 0 555 1527
Size: 555 972
SphI G_CATG'C
Cuts at: 0 1337 1527
Size: 1337 190
SspI AAT'ATT
Cuts at: 0 434 726 1527
Size: 434 292 801
Fragments arranged by size:
801 434 292
StyI C'CwwG_G
Cuts at: 0 1080 1527
Size: 1080 447
TatI w'GTAC_w
Cuts at: 0 1010 1527
Size: 1010 517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 1527
Size: 1369 32 126
Fragments arranged by size:
1369 126 32
XmnI GAAnn'nnTTC
Cuts at: 0 1051 1072 1527
Size: 1051 21 455
Fragments arranged by size:
1051 455 21
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI ApoI BaeI
BamHI BanII BbsI BbvCI BglII Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaBI BsaWI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BsrDI BsrGI BstXI BstYI BtsI DraI
EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII MmeI
MscI MslI MspA1I NdeI NspI PfoI PspOMI PvuII
SexAI SfcI SmlI SphI SspI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI AvrII
BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmrI BmtI BplI BsaI BsaAI BsaHI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI