Restriction map for
pFN21AE1950
(Linear) (Six-base) MAPSORT of: KIEE1950.seq Check: 6924 from: 1 to: 1521
KIEE1950.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AclI AA'CG_TT
Cuts at: 0 1412 1521
Size: 1412 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 878 1460 1521
Size: 694 184 582 61
Fragments arranged by size:
694 582 184 61
AflIII A'CryG_T
Cuts at: 0 1377 1521
Size: 1377 144
AhdI GACnn_n'nnGTC
Cuts at: 0 487 1521
Size: 487 1034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 170 1521
Size: 170 1351
AlwNI CAG_nnn'CTG
Cuts at: 0 941 1521
Size: 941 580
ApoI r'AATT_y
Cuts at: 0 453 1521
Size: 453 1068
AseI AT'TA_AT
Cuts at: 0 404 473 598 1521
Size: 404 69 125 923
Fragments arranged by size:
923 404 125 69
BamHI G'GATC_C
Cuts at: 0 1240 1521
Size: 1240 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1414 1521
Size: 359 1055 107
Fragments arranged by size:
1055 359 107
BciVI GTATCCnnnnn_n'
Cuts at: 0 1389 1521
Size: 1389 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 998 1521
Size: 998 523
Bpu10I CC'TnA_GC
Cuts at: 0 934 1521
Size: 934 587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1319 1442 1521
Size: 650 669 123 79
Fragments arranged by size:
669 650 123 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 197 1521
Size: 197 1324
BsaAI yAC'GTr
Cuts at: 0 1380 1450 1521
Size: 1380 70 71
Fragments arranged by size:
1380 71 70
BsaBI GATnn'nnATC
Cuts at: 0 1052 1521
Size: 1052 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 113 143 1521
Size: 113 30 1378
Fragments arranged by size:
1378 113 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1284 1521
Size: 1284 237
BsmI GAATG_Cn'
Cuts at: 0 1376 1521
Size: 1376 145
BspHI T'CATG_A
Cuts at: 0 48 1521
Size: 48 1473
BsrDI GCAATG_nn'
Cuts at: 0 711 845 1085 1516 1521
Size: 711 134 240 431 5
Fragments arranged by size:
711 431 240 134 5
BsrGI T'GTAC_A
Cuts at: 0 66 1521
Size: 66 1455
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1042 1521
Size: 1042 479
BstYI r'GATC_y
Cuts at: 0 1240 1521
Size: 1240 281
BtgI C'CryG_G
Cuts at: 0 207 373 1521
Size: 207 166 1148
Fragments arranged by size:
1148 207 166
ClaI AT'CG_AT
Cuts at: 0 497 1521
Size: 497 1024
DraI TTT'AAA
Cuts at: 0 417 1521
Size: 417 1104
EarI CTCTTCn'nnn_
Cuts at: 0 854 1243 1521
Size: 854 389 278
Fragments arranged by size:
854 389 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 878 1460 1521
Size: 694 184 582 61
Fragments arranged by size:
694 582 184 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1310 1521
Size: 1310 211
EcoRI G'AATT_C
Cuts at: 0 453 1521
Size: 453 1068
EcoRV GAT'ATC
Cuts at: 0 1167 1521
Size: 1167 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 473 505 574 601 606 628 633 660
Size: 473 32 69 27 5 22 5 27
Cuts at: 660 1521
Size: 861
Fragments arranged by size:
861 473 69 32 27 27 22 5
5
HindIII A'AGCT_T
Cuts at: 0 1460 1521
Size: 1460 61
MslI CAynn'nnrTG
Cuts at: 0 232 827 1521
Size: 232 595 694
Fragments arranged by size:
694 595 232
MspA1I CmG'CkG
Cuts at: 0 154 1521
Size: 154 1367
NcoI C'CATG_G
Cuts at: 0 373 1521
Size: 373 1148
NspI r_CATG'y
Cuts at: 0 692 1521
Size: 692 829
PflMI CCAn_nnn'nTGG
Cuts at: 0 982 1019 1521
Size: 982 37 502
Fragments arranged by size:
982 502 37
PstI C_TGCA'G
Cuts at: 0 159 1434 1521
Size: 159 1275 87
Fragments arranged by size:
1275 159 87
PvuII CAG'CTG
Cuts at: 0 154 1521
Size: 154 1367
SfcI C'TryA_G
Cuts at: 0 155 1430 1521
Size: 155 1275 91
Fragments arranged by size:
1275 155 91
SmlI C'TyrA_G
Cuts at: 0 665 1334 1457 1521
Size: 665 669 123 64
Fragments arranged by size:
669 665 123 64
SnaBI TAC'GTA
Cuts at: 0 1450 1521
Size: 1450 71
StyI C'CwwG_G
Cuts at: 0 373 1355 1521
Size: 373 982 166
Fragments arranged by size:
982 373 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1395 1521
Size: 1395 126
TatI w'GTAC_w
Cuts at: 0 66 1323 1521
Size: 66 1257 198
Fragments arranged by size:
1257 198 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 1521
Size: 798 723
XmnI GAAnn'nnTTC
Cuts at: 0 335 1521
Size: 335 1186
Enzymes that do cut:
AclI AcuI AflIII AhdI AlfI AlwNI ApoI AseI
BamHI BbsI BciVI BmrI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BsgI BsmI BspHI BsrDI BsrGI BstXI
BstYI BtgI ClaI DraI EarI Eco57MI EcoNI EcoRI
EcoRV Hin4I HindIII MslI MspA1I NcoI NspI PflMI
PstI PvuII SfcI SmlI SnaBI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpmI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoO109I FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI