Restriction map for
pFN21AE1949
(Linear) (Six-base) MAPSORT of: KIEE1949.seq Check: 4927 from: 1 to: 1512
KIEE1949.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 585 678 1512
Size: 585 93 834
Fragments arranged by size:
834 585 93
AfeI AGC'GCT
Cuts at: 0 777 1512
Size: 777 735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 585 1512
Size: 585 927
AlwNI CAG_nnn'CTG
Cuts at: 0 249 831 1052 1512
Size: 249 582 221 460
Fragments arranged by size:
582 460 249 221
ApaLI G'TGCA_C
Cuts at: 0 560 1512
Size: 560 952
ApoI r'AATT_y
Cuts at: 0 482 1512
Size: 482 1030
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1144 1177 1512
Size: 1144 33 335
Fragments arranged by size:
1144 335 33
BanII G_rGCy'C
Cuts at: 0 263 644 1135 1263 1439 1512
Size: 263 381 491 128 176 73
Fragments arranged by size:
491 381 263 176 128 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 192 1512
Size: 192 1320
BbvCI CC'TCA_GC
Cuts at: 0 419 1512
Size: 419 1093
BglI GCCn_nnn'nGGC
Cuts at: 0 508 889 1512
Size: 508 381 623
Fragments arranged by size:
623 508 381
BlpI GC'TnA_GC
Cuts at: 0 90 1512
Size: 90 1422
Bme1580I G_kGCm'C
Cuts at: 0 130 564 825 1512
Size: 130 434 261 687
Fragments arranged by size:
687 434 261 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 376 1167 1347 1491 1512
Size: 373 3 791 180 144 21
Fragments arranged by size:
791 373 180 144 21 3
Bpu10I CC'TnA_GC
Cuts at: 0 419 1512
Size: 419 1093
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 448 475 1512
Size: 145 303 27 1037
Fragments arranged by size:
1037 303 145 27
BsaHI Gr'CG_yC
Cuts at: 0 368 1512
Size: 368 1144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 1142 1172 1512
Size: 859 30 253 30 340
Fragments arranged by size:
859 340 253 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 27 30 357 425 1512
Size: 27 3 327 68 1087
Fragments arranged by size:
1087 327 68 27 3
BseYI C'CCAG_C
Cuts at: 0 242 267 660 1104 1134 1140 1512
Size: 242 25 393 444 30 6 372
Fragments arranged by size:
444 393 372 242 30 25 6
BsiEI CG_ry'CG
Cuts at: 0 1428 1512
Size: 1428 84
BsiHKAI G_wGCw'C
Cuts at: 0 263 290 564 644 718 1198 1512
Size: 263 27 274 80 74 480 314
Fragments arranged by size:
480 314 274 263 80 74 27
BsmI GAATG_Cn'
Cuts at: 0 285 1512
Size: 285 1227
Bsp1286I G_dGCh'C
Cuts at: 0 130 263 290 564 644 718 825 1135
Size: 130 133 27 274 80 74 107 310
Cuts at: 1135 1198 1263 1439 1512
Size: 63 65 176 73
Fragments arranged by size:
310 274 176 133 130 107 80 74
73 65 63 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 585 678 1512
Size: 68 517 93 834
Fragments arranged by size:
834 517 93 68
BsrBI CCG'CTC
Cuts at: 0 1388 1512
Size: 1388 124
BsrGI T'GTAC_A
Cuts at: 0 875 1512
Size: 875 637
BssSI C'ACGA_G
Cuts at: 0 1063 1512
Size: 1063 449
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 1512
Size: 429 1083
BstXI CCAn_nnnn'nTGG
Cuts at: 0 79 237 904 1512
Size: 79 158 667 608
Fragments arranged by size:
667 608 158 79
BstYI r'GATC_y
Cuts at: 0 449 1512
Size: 449 1063
BtgI C'CryG_G
Cuts at: 0 497 816 897 1482 1512
Size: 497 319 81 585 30
Fragments arranged by size:
585 497 319 81 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 783 946 1512
Size: 783 163 566
Fragments arranged by size:
783 566 163
BtsI GCAGTG_nn'
Cuts at: 0 980 1512
Size: 980 532
EaeI y'GGCC_r
Cuts at: 0 500 1512
Size: 500 1012
EarI CTCTTCn'nnn_
Cuts at: 0 649 1387 1512
Size: 649 738 125
Fragments arranged by size:
738 649 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 738 1512
Size: 738 774
EcoICRI GAG'CTC
Cuts at: 0 261 642 1512
Size: 261 381 870
Fragments arranged by size:
870 381 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 376 1167 1347 1491 1512
Size: 373 3 791 180 144 21
Fragments arranged by size:
791 373 180 144 21 3
EcoO109I rG'GnC_Cy
Cuts at: 0 882 1512
Size: 882 630
HaeII r_GCGC'y
Cuts at: 0 779 1190 1512
Size: 779 411 322
Fragments arranged by size:
779 411 322
HincII GTy'rAC
Cuts at: 0 1254 1512
Size: 1254 258
MfeI C'AATT_G
Cuts at: 0 400 1512
Size: 400 1112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 845 1512
Size: 122 723 667
Fragments arranged by size:
723 667 122
MslI CAynn'nnrTG
Cuts at: 0 567 1512
Size: 567 945
MspA1I CmG'CkG
Cuts at: 0 155 246 276 429 606 660 831 1104
Size: 155 91 30 153 177 54 171 273
Cuts at: 1104 1144 1512
Size: 40 368
Fragments arranged by size:
368 273 177 171 155 153 91 54
40 30
NcoI C'CATG_G
Cuts at: 0 497 816 897 1482 1512
Size: 497 319 81 585 30
Fragments arranged by size:
585 497 319 81 30
PasI CC'CwG_GG
Cuts at: 0 1239 1512
Size: 1239 273
PfoI T'CCnGG_A
Cuts at: 0 350 1512
Size: 350 1162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1512
Size: 1081 32 399
Fragments arranged by size:
1081 399 32
PstI C_TGCA'G
Cuts at: 0 29 323 1149 1331 1512
Size: 29 294 826 182 181
Fragments arranged by size:
826 294 182 181 29
PvuII CAG'CTG
Cuts at: 0 155 276 429 606 660 1144 1512
Size: 155 121 153 177 54 484 368
Fragments arranged by size:
484 368 177 155 153 121 54
SacI G_AGCT'C
Cuts at: 0 263 644 1512
Size: 263 381 868
Fragments arranged by size:
868 381 263
SapI GCTCTTCn'nnn_
Cuts at: 0 649 1512
Size: 649 863
SfcI C'TryA_G
Cuts at: 0 25 319 1145 1327 1512
Size: 25 294 826 182 185
Fragments arranged by size:
826 294 185 182 25
SmlI C'TyrA_G
Cuts at: 0 160 454 463 1512
Size: 160 294 9 1049
Fragments arranged by size:
1049 294 160 9
StuI AGG'CCT
Cuts at: 0 325 1379 1512
Size: 325 1054 133
Fragments arranged by size:
1054 325 133
StyI C'CwwG_G
Cuts at: 0 371 497 816 897 1482 1512
Size: 371 126 319 81 585 30
Fragments arranged by size:
585 371 319 126 81 30
TatI w'GTAC_w
Cuts at: 0 875 1512
Size: 875 637
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 687 719 1142 1174 1255 1287 1512
Size: 687 32 423 32 81 32 225
Fragments arranged by size:
687 423 225 81 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 152 504 1512
Size: 152 352 1008
Fragments arranged by size:
1008 352 152
Enzymes that do cut:
AarI AfeI AlfI AlwNI ApaLI ApoI BaeI BanII
BbsI BbvCI BglI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII HincII MfeI MmeI MslI MspA1I NcoI PasI
PfoI PsrI PstI PvuII SacI SapI SfcI SmlI
StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI
BsaWI BsgI BsiWI BsmBI BspEI BspHI BsrDI BsrFI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI