Restriction map for
pFN21AE1948
(Linear) (Six-base) MAPSORT of: KIEE1948.seq Check: 8712 from: 1 to: 1509
KIEE1948.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
Acc65I G'GTAC_C
Cuts at: 0 1425 1509
Size: 1425 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1352 1509
Size: 1076 276 157
Fragments arranged by size:
1076 276 157
AhdI GACnn_n'nnGTC
Cuts at: 0 1404 1509
Size: 1404 105
ApoI r'AATT_y
Cuts at: 0 562 1390 1509
Size: 562 828 119
Fragments arranged by size:
828 562 119
BanI G'GyrC_C
Cuts at: 0 43 1241 1425 1509
Size: 43 1198 184 84
Fragments arranged by size:
1198 184 84 43
BbeI G_GCGC'C
Cuts at: 0 47 1245 1509
Size: 47 1198 264
Fragments arranged by size:
1198 264 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 38 72 1509
Size: 38 34 1437
Fragments arranged by size:
1437 38 34
BglII A'GATC_T
Cuts at: 0 749 1509
Size: 749 760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 535 1509
Size: 97 438 974
Fragments arranged by size:
974 438 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1060 1509
Size: 750 310 449
Fragments arranged by size:
750 449 310
BsaHI Gr'CG_yC
Cuts at: 0 44 1242 1509
Size: 44 1198 267
Fragments arranged by size:
1198 267 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 650 1509
Size: 42 608 859
Fragments arranged by size:
859 608 42
BsmI GAATG_Cn'
Cuts at: 0 324 1166 1348 1441 1509
Size: 324 842 182 93 68
Fragments arranged by size:
842 324 182 93 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 1509
Size: 114 1395
BsrDI GCAATG_nn'
Cuts at: 0 425 872 1084 1509
Size: 425 447 212 425
Fragments arranged by size:
447 425 425 212
BstBI TT'CG_AA
Cuts at: 0 1373 1509
Size: 1373 136
BstXI CCAn_nnnn'nTGG
Cuts at: 0 311 1509
Size: 311 1198
BstYI r'GATC_y
Cuts at: 0 300 749 909 1509
Size: 300 449 160 600
Fragments arranged by size:
600 449 300 160
Bsu36I CC'TnA_GG
Cuts at: 0 35 1509
Size: 35 1474
BtsI GCAGTG_nn'
Cuts at: 0 59 645 662 1509
Size: 59 586 17 847
Fragments arranged by size:
847 586 59 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 108 143 1509
Size: 108 35 1366
Fragments arranged by size:
1366 108 35
EaeI y'GGCC_r
Cuts at: 0 116 958 1509
Size: 116 842 551
Fragments arranged by size:
842 551 116
EarI CTCTTCn'nnn_
Cuts at: 0 461 1503 1509
Size: 461 1042 6
Fragments arranged by size:
1042 461 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1217 1509
Size: 1217 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 535 1076 1352 1509
Size: 97 438 541 276 157
Fragments arranged by size:
541 438 276 157 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1468 1509
Size: 1468 41
HaeII r_GCGC'y
Cuts at: 0 47 1245 1509
Size: 47 1198 264
Fragments arranged by size:
1198 264 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 760 792 812 844 1390 1422
Size: 10 32 718 32 20 32 546 32
Cuts at: 1422 1509
Size: 87
Fragments arranged by size:
718 546 87 32 32 32 32 20
10
HincII GTy'rAC
Cuts at: 0 1364 1509
Size: 1364 145
KasI G'GCGC_C
Cuts at: 0 43 1241 1509
Size: 43 1198 268
Fragments arranged by size:
1198 268 43
KpnI G_GTAC'C
Cuts at: 0 1429 1509
Size: 1429 80
MfeI C'AATT_G
Cuts at: 0 587 1509
Size: 587 922
MscI TGG'CCA
Cuts at: 0 960 1509
Size: 960 549
MslI CAynn'nnrTG
Cuts at: 0 764 1298 1509
Size: 764 534 211
Fragments arranged by size:
764 534 211
MspA1I CmG'CkG
Cuts at: 0 486 838 1509
Size: 486 352 671
Fragments arranged by size:
671 486 352
NarI GG'CG_CC
Cuts at: 0 44 1242 1509
Size: 44 1198 267
Fragments arranged by size:
1198 267 44
NdeI CA'TA_TG
Cuts at: 0 1474 1509
Size: 1474 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 919 1509
Size: 919 590
PfoI T'CCnGG_A
Cuts at: 0 303 1509
Size: 303 1206
PsiI TTA'TAA
Cuts at: 0 292 1509
Size: 292 1217
PstI C_TGCA'G
Cuts at: 0 201 640 1035 1509
Size: 201 439 395 474
Fragments arranged by size:
474 439 395 201
PvuII CAG'CTG
Cuts at: 0 486 838 1509
Size: 486 352 671
Fragments arranged by size:
671 486 352
SfcI C'TryA_G
Cuts at: 0 197 636 1031 1509
Size: 197 439 395 478
Fragments arranged by size:
478 439 395 197
SfoI GGC'GCC
Cuts at: 0 45 1243 1509
Size: 45 1198 266
Fragments arranged by size:
1198 266 45
SmlI C'TyrA_G
Cuts at: 0 729 1039 1509
Size: 729 310 470
Fragments arranged by size:
729 470 310
SspI AAT'ATT
Cuts at: 0 269 1509
Size: 269 1240
StuI AGG'CCT
Cuts at: 0 658 1037 1509
Size: 658 379 472
Fragments arranged by size:
658 472 379
StyI C'CwwG_G
Cuts at: 0 1283 1421 1452 1509
Size: 1283 138 31 57
Fragments arranged by size:
1283 138 57 31
Tth111I GACn'n_nGTC
Cuts at: 0 1359 1509
Size: 1359 150
XmnI GAAnn'nnTTC
Cuts at: 0 548 563 1509
Size: 548 15 946
Fragments arranged by size:
946 548 15
Enzymes that do cut:
Acc65I AcuI AhdI ApoI BanI BbeI BcgI BglII
BpmI BpuEI BsaHI BsgI BsmI BspMI BsrDI BstBI
BstXI BstYI Bsu36I BtsI CspCI EaeI EarI EciI
Eco57MI FalI HaeII Hin4I HincII KasI KpnI MfeI
MscI MslI MspA1I NarI NdeI PflMI PfoI PsiI
PstI PvuII SfcI SfoI SmlI SspI StuI StyI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbsI BbvCI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstZ17I BtgI BtgZI ClaI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HindIII HpaI MluI MmeI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmaI ZraI