Restriction map for
pFN21AE1947
(Linear) (Six-base) MAPSORT of: KIEE1947.seq Check: 2818 from: 1 to: 1503
KIEE1947.orf
With 182 enzymes: SgfI *
April 22, 2018 20:12 ..
AccI GT'mk_AC
Cuts at: 0 751 1503
Size: 751 752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1503
Size: 695 808
AfeI AGC'GCT
Cuts at: 0 1254 1503
Size: 1254 249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 97 1503
Size: 97 1406
AlwNI CAG_nnn'CTG
Cuts at: 0 746 819 1503
Size: 746 73 684
Fragments arranged by size:
746 684 73
ApaI G_GGCC'C
Cuts at: 0 1227 1503
Size: 1227 276
ApaLI G'TGCA_C
Cuts at: 0 1264 1503
Size: 1264 239
AvaI C'yCGr_G
Cuts at: 0 131 151 1503
Size: 131 20 1352
Fragments arranged by size:
1352 131 20
BanI G'GyrC_C
Cuts at: 0 61 106 424 871 1000 1503
Size: 61 45 318 447 129 503
Fragments arranged by size:
503 447 318 129 61 45
BanII G_rGCy'C
Cuts at: 0 152 161 1227 1503
Size: 152 9 1066 276
Fragments arranged by size:
1066 276 152 9
BbeI G_GCGC'C
Cuts at: 0 110 1503
Size: 110 1393
BbsI GAAGACnn'nnnn_
Cuts at: 0 1328 1503
Size: 1328 175
BciVI GTATCCnnnnn_n'
Cuts at: 0 270 1503
Size: 270 1233
BclI T'GATC_A
Cuts at: 0 704 785 1503
Size: 704 81 718
Fragments arranged by size:
718 704 81
BglI GCCn_nnn'nGGC
Cuts at: 0 104 170 1503
Size: 104 66 1333
Fragments arranged by size:
1333 104 66
Bme1580I G_kGCm'C
Cuts at: 0 392 427 1174 1227 1268 1503
Size: 392 35 747 53 41 235
Fragments arranged by size:
747 392 235 53 41 35
BmgBI CAC'GTC
Cuts at: 0 1124 1503
Size: 1124 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 1018 1503
Size: 534 484 485
Fragments arranged by size:
534 485 484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1503
Size: 601 902
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 1503
Size: 130 1373
BsaAI yAC'GTr
Cuts at: 0 228 1503
Size: 228 1275
BsaHI Gr'CG_yC
Cuts at: 0 107 1503
Size: 107 1396
BsaWI w'CCGG_w
Cuts at: 0 371 1503
Size: 371 1132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 616 646 1503
Size: 616 30 857
Fragments arranged by size:
857 616 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 927 1503
Size: 169 758 576
Fragments arranged by size:
758 576 169
BseYI C'CCAG_C
Cuts at: 0 65 420 1503
Size: 65 355 1083
Fragments arranged by size:
1083 355 65
BsiEI CG_ry'CG
Cuts at: 0 165 1503
Size: 165 1338
BsiHKAI G_wGCw'C
Cuts at: 0 1268 1503
Size: 1268 235
Bsp1286I G_dGCh'C
Cuts at: 0 152 161 392 427 1174 1227 1268 1503
Size: 152 9 231 35 747 53 41 235
Fragments arranged by size:
747 235 231 152 53 41 35 9
BspEI T'CCGG_A
Cuts at: 0 371 1503
Size: 371 1132
BspHI T'CATG_A
Cuts at: 0 1364 1503
Size: 1364 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1106 1503
Size: 1106 397
BsrDI GCAATG_nn'
Cuts at: 0 476 1503
Size: 476 1027
BsrGI T'GTAC_A
Cuts at: 0 731 1503
Size: 731 772
BstAPI GCAn_nnn'nTGC
Cuts at: 0 304 1098 1503
Size: 304 794 405
Fragments arranged by size:
794 405 304
BstEII G'GTnAC_C
Cuts at: 0 1053 1503
Size: 1053 450
BstYI r'GATC_y
Cuts at: 0 816 1503
Size: 816 687
BstZ17I GTA'TAC
Cuts at: 0 752 1503
Size: 752 751
Bsu36I CC'TnA_GG
Cuts at: 0 199 1503
Size: 199 1304
BtgI C'CryG_G
Cuts at: 0 57 239 447 941 1166 1503
Size: 57 182 208 494 225 337
Fragments arranged by size:
494 337 225 208 182 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 313 1503
Size: 313 1190
BtsI GCAGTG_nn'
Cuts at: 0 305 1503
Size: 305 1198
DrdI GACnn_nn'nnGTC
Cuts at: 0 1353 1503
Size: 1353 150
EaeI y'GGCC_r
Cuts at: 0 162 1102 1118 1503
Size: 162 940 16 385
Fragments arranged by size:
940 385 162 16
EagI C'GGCC_G
Cuts at: 0 162 1503
Size: 162 1341
EarI CTCTTCn'nnn_
Cuts at: 0 140 634 1408 1503
Size: 140 494 774 95
Fragments arranged by size:
774 494 140 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 695 1503
Size: 601 94 808
Fragments arranged by size:
808 601 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1503
Size: 373 1130
EcoO109I rG'GnC_Cy
Cuts at: 0 366 1297 1503
Size: 366 931 206
Fragments arranged by size:
931 366 206
FspI TGC'GCA
Cuts at: 0 91 1503
Size: 91 1412
HaeII r_GCGC'y
Cuts at: 0 110 1256 1443 1503
Size: 110 1146 187 60
Fragments arranged by size:
1146 187 110 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1175 1207 1474 1503
Size: 1175 32 267 29
Fragments arranged by size:
1175 267 32 29
HincII GTy'rAC
Cuts at: 0 1358 1503
Size: 1358 145
KasI G'GCGC_C
Cuts at: 0 106 1503
Size: 106 1397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 672 1503
Size: 545 127 831
Fragments arranged by size:
831 545 127
MscI TGG'CCA
Cuts at: 0 1120 1503
Size: 1120 383
MslI CAynn'nnrTG
Cuts at: 0 271 1177 1503
Size: 271 906 326
Fragments arranged by size:
906 326 271
MspA1I CmG'CkG
Cuts at: 0 95 348 981 1288 1451 1503
Size: 95 253 633 307 163 52
Fragments arranged by size:
633 307 253 163 95 52
NarI GG'CG_CC
Cuts at: 0 107 1503
Size: 107 1396
NcoI C'CATG_G
Cuts at: 0 447 1503
Size: 447 1056
PasI CC'CwG_GG
Cuts at: 0 114 399 1503
Size: 114 285 1104
Fragments arranged by size:
1104 285 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 1010 1503
Size: 1010 493
PpuMI rG'GwC_Cy
Cuts at: 0 366 1503
Size: 366 1137
PspOMI G'GGCC_C
Cuts at: 0 1223 1503
Size: 1223 280
PstI C_TGCA'G
Cuts at: 0 641 915 1503
Size: 641 274 588
Fragments arranged by size:
641 588 274
PvuII CAG'CTG
Cuts at: 0 348 981 1503
Size: 348 633 522
Fragments arranged by size:
633 522 348
SapI GCTCTTCn'nnn_
Cuts at: 0 140 1503
Size: 140 1363
SexAI A'CCwGG_T
Cuts at: 0 431 1503
Size: 431 1072
SfcI C'TryA_G
Cuts at: 0 637 911 1503
Size: 637 274 592
Fragments arranged by size:
637 592 274
SfoI GGC'GCC
Cuts at: 0 108 1503
Size: 108 1395
SmaI CCC'GGG
Cuts at: 0 133 1503
Size: 133 1370
StuI AGG'CCT
Cuts at: 0 567 1503
Size: 567 936
StyI C'CwwG_G
Cuts at: 0 447 1009 1310 1503
Size: 447 562 301 193
Fragments arranged by size:
562 447 301 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 154 292 412 771 1036 1503
Size: 79 75 138 120 359 265 467
Fragments arranged by size:
467 359 265 138 120 79 75
TatI w'GTAC_w
Cuts at: 0 731 839 1503
Size: 731 108 664
Fragments arranged by size:
731 664 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1503
Size: 1068 32 403
Fragments arranged by size:
1068 403 32
XmaI C'CCGG_G
Cuts at: 0 131 1503
Size: 131 1372
XmnI GAAnn'nnTTC
Cuts at: 0 1018 1503
Size: 1018 485
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BciVI BclI BglI Bme1580I
BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NcoI PasI PflMI PpuMI PspOMI
PstI PvuII SapI SexAI SfcI SfoI SmaI StuI
StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BfrBI BglII BlpI BmtI BplI
Bpu10I BpuEI BsaBI BsgI BsiWI BsmI BsmBI BsrBI
BsrFI BssHII BssSI BstBI BstXI ClaI CspCI DraI
DraIII EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI ZraI