Restriction map for
pFN21AE1944
(Linear) (Six-base) MAPSORT of: KIEE1944.seq Check: 882 from: 1 to: 1500
KIEE1944.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1120 1486 1500
Size: 964 156 366 14
Fragments arranged by size:
964 366 156 14
AfeI AGC'GCT
Cuts at: 0 239 1220 1500
Size: 239 981 280
Fragments arranged by size:
981 280 239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1336 1500
Size: 1336 164
AlwNI CAG_nnn'CTG
Cuts at: 0 744 1266 1425 1500
Size: 744 522 159 75
Fragments arranged by size:
744 522 159 75
ApaI G_GGCC'C
Cuts at: 0 395 536 679 1500
Size: 395 141 143 821
Fragments arranged by size:
821 395 143 141
ApaLI G'TGCA_C
Cuts at: 0 1311 1500
Size: 1311 189
AvaI C'yCGr_G
Cuts at: 0 28 61 288 505 679 1500
Size: 28 33 227 217 174 821
Fragments arranged by size:
821 227 217 174 33 28
BamHI G'GATC_C
Cuts at: 0 375 1500
Size: 375 1125
BanI G'GyrC_C
Cuts at: 0 314 1201 1279 1500
Size: 314 887 78 221
Fragments arranged by size:
887 314 221 78
BanII G_rGCy'C
Cuts at: 0 62 281 395 536 679 736 1500
Size: 62 219 114 141 143 57 764
Fragments arranged by size:
764 219 143 141 114 62 57
BbeI G_GCGC'C
Cuts at: 0 318 1500
Size: 318 1182
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 1500
Size: 608 892
BbvCI CC'TCA_GC
Cuts at: 0 686 737 1500
Size: 686 51 763
Fragments arranged by size:
763 686 51
BglI GCCn_nnn'nGGC
Cuts at: 0 958 1500
Size: 958 542
BglII A'GATC_T
Cuts at: 0 1469 1500
Size: 1469 31
BlpI GC'TnA_GC
Cuts at: 0 730 1500
Size: 730 770
Bme1580I G_kGCm'C
Cuts at: 0 395 536 679 936 963 1029 1204 1284
Size: 395 141 143 257 27 66 175 80
Cuts at: 1284 1315 1500
Size: 31 185
Fragments arranged by size:
395 257 185 175 143 141 80 66
31 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 531 1500
Size: 531 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 506 1072 1255 1500
Size: 388 118 566 183 245
Fragments arranged by size:
566 388 245 183 118
Bpu10I CC'TnA_GC
Cuts at: 0 686 737 1500
Size: 686 51 763
Fragments arranged by size:
763 686 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 1348 1500
Size: 1348 152
BsaAI yAC'GTr
Cuts at: 0 720 1500
Size: 720 780
BsaHI Gr'CG_yC
Cuts at: 0 315 1414 1500
Size: 315 1099 86
Fragments arranged by size:
1099 315 86
BsaWI w'CCGG_w
Cuts at: 0 76 97 160 1500
Size: 76 21 63 1340
Fragments arranged by size:
1340 76 63 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 504 534 552 582 1500
Size: 445 30 29 30 18 30 918
Fragments arranged by size:
918 445 30 30 30 29 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 194 466 630 633 1500
Size: 191 3 272 164 3 867
Fragments arranged by size:
867 272 191 164 3 3
BseYI C'CCAG_C
Cuts at: 0 220 551 669 813 1500
Size: 220 331 118 144 687
Fragments arranged by size:
687 331 220 144 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 1207 1213 1306 1500
Size: 543 664 6 93 194
Fragments arranged by size:
664 543 194 93 6
BsiEI CG_ry'CG
Cuts at: 0 400 1500
Size: 400 1100
BsiHKAI G_wGCw'C
Cuts at: 0 1315 1500
Size: 1315 185
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1405 1500
Size: 1405 95
Bsp1286I G_dGCh'C
Cuts at: 0 62 281 395 536 679 736 936 963
Size: 62 219 114 141 143 57 200 27
Cuts at: 963 1029 1204 1284 1315 1500
Size: 66 175 80 31 185
Fragments arranged by size:
219 200 185 175 143 141 114 80
66 62 57 31 27
BspEI T'CCGG_A
Cuts at: 0 76 97 160 1500
Size: 76 21 63 1340
Fragments arranged by size:
1340 76 63 21
BspHI T'CATG_A
Cuts at: 0 804 1500
Size: 804 696
BsrBI CCG'CTC
Cuts at: 0 321 618 1248 1500
Size: 321 297 630 252
Fragments arranged by size:
630 321 297 252
BsrDI GCAATG_nn'
Cuts at: 0 1124 1500
Size: 1124 376
BsrFI r'CCGG_y
Cuts at: 0 265 399 971 1500
Size: 265 134 572 529
Fragments arranged by size:
572 529 265 134
BsrGI T'GTAC_A
Cuts at: 0 903 1500
Size: 903 597
BssHII G'CGCG_C
Cuts at: 0 261 1500
Size: 261 1239
BstYI r'GATC_y
Cuts at: 0 375 1469 1500
Size: 375 1094 31
Fragments arranged by size:
1094 375 31
BtgI C'CryG_G
Cuts at: 0 304 410 1500
Size: 304 106 1090
Fragments arranged by size:
1090 304 106
BtsI GCAGTG_nn'
Cuts at: 0 803 1500
Size: 803 697
DraIII CAC_nnn'GTG
Cuts at: 0 1367 1500
Size: 1367 133
DrdI GACnn_nn'nnGTC
Cuts at: 0 784 1500
Size: 784 716
EaeI y'GGCC_r
Cuts at: 0 232 397 401 1048 1335 1500
Size: 232 165 4 647 287 165
Fragments arranged by size:
647 287 232 165 165 4
EagI C'GGCC_G
Cuts at: 0 397 1500
Size: 397 1103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 375 463 1500
Size: 375 88 1037
Fragments arranged by size:
1037 375 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 506 964 1072 1120 1255 1486 1500
Size: 388 118 458 108 48 135 231 14
Fragments arranged by size:
458 388 231 135 118 108 48 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1270 1429 1500
Size: 1270 159 71
Fragments arranged by size:
1270 159 71
EcoO109I rG'GnC_Cy
Cuts at: 0 88 184 392 533 574 675 676 690
Size: 88 96 208 141 41 101 1 14
Cuts at: 690 810 855 1251 1422 1500
Size: 120 45 396 171 78
Fragments arranged by size:
396 208 171 141 120 101 96 88
78 45 41 14 1
FseI GG_CCGG'CC
Cuts at: 0 403 1500
Size: 403 1097
HaeII r_GCGC'y
Cuts at: 0 107 241 318 730 1011 1222 1500
Size: 107 134 77 412 281 211 278
Fragments arranged by size:
412 281 278 211 134 107 77
KasI G'GCGC_C
Cuts at: 0 314 1500
Size: 314 1186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1500
Size: 401 1099
MscI TGG'CCA
Cuts at: 0 234 1500
Size: 234 1266
MslI CAynn'nnrTG
Cuts at: 0 139 1189 1500
Size: 139 1050 311
Fragments arranged by size:
1050 311 139
MspA1I CmG'CkG
Cuts at: 0 220 306 412 744 817 1031 1092 1333
Size: 220 86 106 332 73 214 61 241
Cuts at: 1333 1500
Size: 167
Fragments arranged by size:
332 241 220 214 167 106 86 73
61
NaeI GCC'GGC
Cuts at: 0 267 401 1500
Size: 267 134 1099
Fragments arranged by size:
1099 267 134
NarI GG'CG_CC
Cuts at: 0 315 1500
Size: 315 1185
NgoMIV G'CCGG_C
Cuts at: 0 265 399 1500
Size: 265 134 1101
Fragments arranged by size:
1101 265 134
NspI r_CATG'y
Cuts at: 0 420 1302 1500
Size: 420 882 198
Fragments arranged by size:
882 420 198
PflMI CCAn_nnn'nTGG
Cuts at: 0 1017 1500
Size: 1017 483
PfoI T'CCnGG_A
Cuts at: 0 570 857 1500
Size: 570 287 643
Fragments arranged by size:
643 570 287
PmlI CAC'GTG
Cuts at: 0 720 1500
Size: 720 780
PpuMI rG'GwC_Cy
Cuts at: 0 184 574 690 855 1422 1500
Size: 184 390 116 165 567 78
Fragments arranged by size:
567 390 184 165 116 78
PspOMI G'GGCC_C
Cuts at: 0 391 532 675 1500
Size: 391 141 143 825
Fragments arranged by size:
825 391 143 141
PvuII CAG'CTG
Cuts at: 0 744 1092 1333 1500
Size: 744 348 241 167
Fragments arranged by size:
744 348 241 167
SacII CC_GC'GG
Cuts at: 0 307 413 1500
Size: 307 106 1087
Fragments arranged by size:
1087 307 106
SanDI GG'GwC_CC
Cuts at: 0 574 1500
Size: 574 926
SfcI C'TryA_G
Cuts at: 0 964 1500
Size: 964 536
SfoI GGC'GCC
Cuts at: 0 316 1500
Size: 316 1184
SmaI CCC'GGG
Cuts at: 0 30 63 290 1500
Size: 30 33 227 1210
Fragments arranged by size:
1210 227 33 30
SphI G_CATG'C
Cuts at: 0 1302 1500
Size: 1302 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 212 577 1500
Size: 212 365 923
Fragments arranged by size:
923 365 212
TatI w'GTAC_w
Cuts at: 0 903 1500
Size: 903 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 504 536 552 584 1500
Size: 504 32 16 32 916
Fragments arranged by size:
916 504 32 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 466 1500
Size: 466 1034
XmaI C'CCGG_G
Cuts at: 0 28 61 288 1500
Size: 28 33 227 1212
Fragments arranged by size:
1212 227 33 28
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstYI
BtgI BtsI DraIII DrdI EaeI EagI EciI Eco57MI
EcoNI EcoO109I FseI HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PmlI
PpuMI PspOMI PvuII SacII SanDI SfcI SfoI SmaI
SphI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI
BpuEI BsaBI BsiWI BsmI BspMI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EarI EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmnI ZraI