Restriction map for
pFN21AE1939
(Linear) (Six-base) MAPSORT of: KIEE1939.seq Check: 4528 from: 1 to: 1443
KIEE1939.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 332 1443
Size: 332 1111
AccI GT'mk_AC
Cuts at: 0 830 1443
Size: 830 613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1225 1378 1443
Size: 1035 190 153 65
Fragments arranged by size:
1035 190 153 65
AfeI AGC'GCT
Cuts at: 0 1277 1443
Size: 1277 166
AflIII A'CryG_T
Cuts at: 0 506 1443
Size: 506 937
AhdI GACnn_n'nnGTC
Cuts at: 0 970 1443
Size: 970 473
AleI CACnn'nnGTG
Cuts at: 0 254 1156 1443
Size: 254 902 287
Fragments arranged by size:
902 287 254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 1443
Size: 1393 32 18
Fragments arranged by size:
1393 32 18
AlwNI CAG_nnn'CTG
Cuts at: 0 300 463 726 1443
Size: 300 163 263 717
Fragments arranged by size:
717 300 263 163
AvaI C'yCGr_G
Cuts at: 0 707 1443
Size: 707 736
BamHI G'GATC_C
Cuts at: 0 200 1443
Size: 200 1243
BanI G'GyrC_C
Cuts at: 0 73 1417 1443
Size: 73 1344 26
Fragments arranged by size:
1344 73 26
BanII G_rGCy'C
Cuts at: 0 21 382 1443
Size: 21 361 1061
Fragments arranged by size:
1061 361 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 846 1125 1443
Size: 846 279 318
Fragments arranged by size:
846 318 279
BbvCI CC'TCA_GC
Cuts at: 0 277 1443
Size: 277 1166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 468 502 1443
Size: 468 34 941
Fragments arranged by size:
941 468 34
BglI GCCn_nnn'nGGC
Cuts at: 0 709 1443
Size: 709 734
Bme1580I G_kGCm'C
Cuts at: 0 1031 1443
Size: 1031 412
BmgBI CAC'GTC
Cuts at: 0 210 495 1443
Size: 210 285 948
Fragments arranged by size:
948 285 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 1205 1443
Size: 1205 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 598 748 758 1000 1101 1443
Size: 453 145 150 10 242 101 342
Fragments arranged by size:
453 342 242 150 145 101 10
Bpu10I CC'TnA_GC
Cuts at: 0 163 277 1443
Size: 163 114 1166
Fragments arranged by size:
1166 163 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1443
Size: 216 1227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 721 751 1443
Size: 721 30 692
Fragments arranged by size:
721 692 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 312 1270 1443
Size: 312 958 173
Fragments arranged by size:
958 312 173
BseYI C'CCAG_C
Cuts at: 0 1111 1443
Size: 1111 332
BsiEI CG_ry'CG
Cuts at: 0 216 1443
Size: 216 1227
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1443
Size: 1106 337
Bsp1286I G_dGCh'C
Cuts at: 0 21 382 1031 1106 1443
Size: 21 361 649 75 337
Fragments arranged by size:
649 361 337 75 21
BspHI T'CATG_A
Cuts at: 0 797 879 1443
Size: 797 82 564
Fragments arranged by size:
797 564 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 332 1443
Size: 332 1111
BsrBI CCG'CTC
Cuts at: 0 1041 1443
Size: 1041 402
BsrDI GCAATG_nn'
Cuts at: 0 40 1443
Size: 40 1403
BstAPI GCAn_nnn'nTGC
Cuts at: 0 590 1443
Size: 590 853
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 1443
Size: 36 1407
BstYI r'GATC_y
Cuts at: 0 200 1391 1443
Size: 200 1191 52
Fragments arranged by size:
1191 200 52
Bsu36I CC'TnA_GG
Cuts at: 0 809 1443
Size: 809 634
BtgI C'CryG_G
Cuts at: 0 216 976 1443
Size: 216 760 467
Fragments arranged by size:
760 467 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 471 1443
Size: 471 972
BtsI GCAGTG_nn'
Cuts at: 0 486 1443
Size: 486 957
DrdI GACnn_nn'nnGTC
Cuts at: 0 954 1443
Size: 954 489
EaeI y'GGCC_r
Cuts at: 0 11 213 291 863 1148 1331 1443
Size: 11 202 78 572 285 183 112
Fragments arranged by size:
572 285 202 183 112 78 11
EagI C'GGCC_G
Cuts at: 0 213 1443
Size: 213 1230
EarI CTCTTCn'nnn_
Cuts at: 0 544 1443
Size: 544 899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1152 1443
Size: 1152 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 598 748 758 1000 1035 1101 1225
Size: 453 145 150 10 242 35 66 124
Cuts at: 1225 1378 1443
Size: 153 65
Fragments arranged by size:
453 242 153 150 145 124 66 65
35 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 447 808 1443
Size: 447 361 635
Fragments arranged by size:
635 447 361
EcoO109I rG'GnC_Cy
Cuts at: 0 5 297 1443
Size: 5 292 1146
Fragments arranged by size:
1146 292 5
EcoRV GAT'ATC
Cuts at: 0 892 1443
Size: 892 551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 1443
Size: 381 1062
FspI TGC'GCA
Cuts at: 0 355 1443
Size: 355 1088
HaeII r_GCGC'y
Cuts at: 0 225 1279 1443
Size: 225 1054 164
Fragments arranged by size:
1054 225 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 956 988 1443
Size: 956 32 455
Fragments arranged by size:
956 455 32
HincII GTy'rAC
Cuts at: 0 420 1443
Size: 420 1023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 966 987 1227 1250 1443
Size: 341 625 21 240 23 193
Fragments arranged by size:
625 341 240 193 23 21
MscI TGG'CCA
Cuts at: 0 13 293 1150 1443
Size: 13 280 857 293
Fragments arranged by size:
857 293 280 13
MslI CAynn'nnrTG
Cuts at: 0 254 1156 1443
Size: 254 902 287
Fragments arranged by size:
902 287 254
MspA1I CmG'CkG
Cuts at: 0 254 473 1443
Size: 254 219 970
Fragments arranged by size:
970 254 219
NspI r_CATG'y
Cuts at: 0 510 1443
Size: 510 933
PasI CC'CwG_GG
Cuts at: 0 1057 1443
Size: 1057 386
PciI A'CATG_T
Cuts at: 0 506 1443
Size: 506 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 1443
Size: 726 717
PfoI T'CCnGG_A
Cuts at: 0 445 1443
Size: 445 998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 1443
Size: 547 32 864
Fragments arranged by size:
864 547 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 753 785 1443
Size: 753 32 658
Fragments arranged by size:
753 658 32
PstI C_TGCA'G
Cuts at: 0 119 152 478 795 1391 1443
Size: 119 33 326 317 596 52
Fragments arranged by size:
596 326 317 119 52 33
PvuII CAG'CTG
Cuts at: 0 254 473 1443
Size: 254 219 970
Fragments arranged by size:
970 254 219
ScaI AGT'ACT
Cuts at: 0 575 1443
Size: 575 868
SfcI C'TryA_G
Cuts at: 0 115 148 373 474 625 791 1387 1443
Size: 115 33 225 101 151 166 596 56
Fragments arranged by size:
596 225 166 151 115 101 56 33
SmlI C'TyrA_G
Cuts at: 0 195 1443
Size: 195 1248
StuI AGG'CCT
Cuts at: 0 282 1443
Size: 282 1161
StyI C'CwwG_G
Cuts at: 0 8 1380 1443
Size: 8 1372 63
Fragments arranged by size:
1372 63 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 405 1405 1443
Size: 61 344 1000 38
Fragments arranged by size:
1000 344 61 38
TatI w'GTAC_w
Cuts at: 0 573 1443
Size: 573 870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 214 1005 1443
Size: 84 130 791 438
Fragments arranged by size:
791 438 130 84
XmnI GAAnn'nnTTC
Cuts at: 0 538 752 1443
Size: 538 214 691
Fragments arranged by size:
691 538 214
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AloI
AlwNI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NspI PasI PciI PflMI PfoI PpiI
PsrI PstI PvuII ScaI SfcI SmlI StuI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BclI BfrBI BglII BlpI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII EcoICRI EcoRI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI ZraI