Restriction map for
pFN21AE1935
(Linear) (Six-base) MAPSORT of: KIEE1935.seq Check: 5336 from: 1 to: 1422
KIEE1935.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AccI GT'mk_AC
Cuts at: 0 1036 1422
Size: 1036 386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 830 1422
Size: 785 45 592
Fragments arranged by size:
785 592 45
AfeI AGC'GCT
Cuts at: 0 1333 1422
Size: 1333 89
AlwNI CAG_nnn'CTG
Cuts at: 0 322 1422
Size: 322 1100
ApaI G_GGCC'C
Cuts at: 0 1168 1422
Size: 1168 254
ApaLI G'TGCA_C
Cuts at: 0 930 1422
Size: 930 492
ApoI r'AATT_y
Cuts at: 0 91 151 394 876 1422
Size: 91 60 243 482 546
Fragments arranged by size:
546 482 243 91 60
AvaI C'yCGr_G
Cuts at: 0 961 1422
Size: 961 461
BanII G_rGCy'C
Cuts at: 0 1168 1422
Size: 1168 254
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 1142 1422
Size: 896 246 280
Fragments arranged by size:
896 280 246
BbvCI CC'TCA_GC
Cuts at: 0 264 1422
Size: 264 1158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 344 378 1422
Size: 344 34 1044
Fragments arranged by size:
1044 344 34
BlpI GC'TnA_GC
Cuts at: 0 697 1422
Size: 697 725
Bme1580I G_kGCm'C
Cuts at: 0 934 1168 1422
Size: 934 234 254
Fragments arranged by size:
934 254 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 152 1422
Size: 152 1270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 822 1422
Size: 822 600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 763 1422
Size: 595 168 659
Fragments arranged by size:
659 595 168
Bpu10I CC'TnA_GC
Cuts at: 0 264 1160 1422
Size: 264 896 262
Fragments arranged by size:
896 264 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1422
Size: 1114 308
BsaAI yAC'GTr
Cuts at: 0 408 1422
Size: 408 1014
BsaWI w'CCGG_w
Cuts at: 0 1068 1422
Size: 1068 354
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 769 799 1330 1360 1422
Size: 769 30 531 30 62
Fragments arranged by size:
769 531 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 1364 1422
Size: 500 864 58
Fragments arranged by size:
864 500 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1241 1422
Size: 318 923 181
Fragments arranged by size:
923 318 181
BsiHKAI G_wGCw'C
Cuts at: 0 934 1422
Size: 934 488
BsmI GAATG_Cn'
Cuts at: 0 1126 1422
Size: 1126 296
Bsp1286I G_dGCh'C
Cuts at: 0 934 1168 1422
Size: 934 234 254
Fragments arranged by size:
934 254 234
BspEI T'CCGG_A
Cuts at: 0 1068 1422
Size: 1068 354
BsrGI T'GTAC_A
Cuts at: 0 140 1422
Size: 140 1282
BstAPI GCAn_nnn'nTGC
Cuts at: 0 274 1422
Size: 274 1148
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1026 1311 1422
Size: 1026 285 111
Fragments arranged by size:
1026 285 111
BstYI r'GATC_y
Cuts at: 0 450 1422
Size: 450 972
BstZ17I GTA'TAC
Cuts at: 0 1037 1422
Size: 1037 385
Bsu36I CC'TnA_GG
Cuts at: 0 1292 1422
Size: 1292 130
BtgI C'CryG_G
Cuts at: 0 914 1304 1422
Size: 914 390 118
Fragments arranged by size:
914 390 118
BtsI GCAGTG_nn'
Cuts at: 0 320 1422
Size: 320 1102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 460 495 1422
Size: 460 35 927
Fragments arranged by size:
927 460 35
EaeI y'GGCC_r
Cuts at: 0 821 1422
Size: 821 601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 763 785 830 1422
Size: 595 168 22 45 592
Fragments arranged by size:
595 592 168 45 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1084 1422
Size: 1084 338
EcoO109I rG'GnC_Cy
Cuts at: 0 9 260 345 560 1164 1165 1422
Size: 9 251 85 215 604 1 257
Fragments arranged by size:
604 257 251 215 85 9 1
EcoRI G'AATT_C
Cuts at: 0 91 394 876 1422
Size: 91 303 482 546
Fragments arranged by size:
546 482 303 91
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 132 1422
Size: 132 1290
HaeII r_GCGC'y
Cuts at: 0 1335 1422
Size: 1335 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 673 705 850 882 1330 1362
Size: 106 32 535 32 145 32 448 32
Cuts at: 1362 1422
Size: 60
Fragments arranged by size:
535 448 145 106 60 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1326 1422
Size: 1326 96
HpaI GTT'AAC
Cuts at: 0 1326 1422
Size: 1326 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1373 1422
Size: 1373 49
MscI TGG'CCA
Cuts at: 0 823 1422
Size: 823 599
MspA1I CmG'CkG
Cuts at: 0 949 1422
Size: 949 473
NcoI C'CATG_G
Cuts at: 0 914 1422
Size: 914 508
NspI r_CATG'y
Cuts at: 0 46 1422
Size: 46 1376
PfoI T'CCnGG_A
Cuts at: 0 1008 1422
Size: 1008 414
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 344 376 1422
Size: 344 32 1046
Fragments arranged by size:
1046 344 32
PpuMI rG'GwC_Cy
Cuts at: 0 9 260 560 1422
Size: 9 251 300 862
Fragments arranged by size:
862 300 251 9
PshAI GACnn'nnGTC
Cuts at: 0 896 1422
Size: 896 526
PspOMI G'GGCC_C
Cuts at: 0 1164 1422
Size: 1164 258
PvuII CAG'CTG
Cuts at: 0 949 1422
Size: 949 473
SmlI C'TyrA_G
Cuts at: 0 1093 1422
Size: 1093 329
SpeI A'CTAG_T
Cuts at: 0 132 1422
Size: 132 1290
SspI AAT'ATT
Cuts at: 0 487 1422
Size: 487 935
StyI C'CwwG_G
Cuts at: 0 914 1113 1422
Size: 914 199 309
Fragments arranged by size:
914 309 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 329 1317 1422
Size: 226 103 988 105
Fragments arranged by size:
988 226 105 103
TatI w'GTAC_w
Cuts at: 0 140 1250 1422
Size: 140 1110 172
Fragments arranged by size:
1110 172 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 380 412 1422
Size: 380 32 1010
Fragments arranged by size:
1010 380 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 1422
Size: 345 1077
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaI ApaLI ApoI AvaI
BanII BbsI BbvCI BcgI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspEI BsrGI BstAPI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI CspCI EaeI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI MmeI
MscI MspA1I NcoI NspI PfoI PpiI PpuMI PshAI
PspOMI PvuII SmlI SpeI SspI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BclI BfrBI BglI BglII
BmgBI BmtI BsaI BsaBI BsaHI BseYI BsiEI BsiWI
BsmBI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BtgZI ClaI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoRV FseI FspI FspAI HindIII
KasI KpnI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI