Restriction map for
pFN21AE1933
(Linear) (Six-base) MAPSORT of: KIEE1933.seq Check: 5587 from: 1 to: 1410
KIEE1933.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1100 1410
Size: 1100 310
AccI GT'mk_AC
Cuts at: 0 470 1410
Size: 470 940
Acc65I G'GTAC_C
Cuts at: 0 55 1410
Size: 55 1355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 644 1247 1325 1410
Size: 152 492 603 78 85
Fragments arranged by size:
603 492 152 85 78
AflIII A'CryG_T
Cuts at: 0 857 1410
Size: 857 553
AhdI GACnn_n'nnGTC
Cuts at: 0 116 1410
Size: 116 1294
AleI CACnn'nnGTG
Cuts at: 0 749 1410
Size: 749 661
AlwNI CAG_nnn'CTG
Cuts at: 0 138 508 786 928 1410
Size: 138 370 278 142 482
Fragments arranged by size:
482 370 278 142 138
AvaI C'yCGr_G
Cuts at: 0 486 842 1410
Size: 486 356 568
Fragments arranged by size:
568 486 356
BanI G'GyrC_C
Cuts at: 0 25 55 664 833 1410
Size: 25 30 609 169 577
Fragments arranged by size:
609 577 169 30 25
BanII G_rGCy'C
Cuts at: 0 18 869 1410
Size: 18 851 541
Fragments arranged by size:
851 541 18
BbeI G_GCGC'C
Cuts at: 0 837 1410
Size: 837 573
BglI GCCn_nnn'nGGC
Cuts at: 0 682 1410
Size: 682 728
BlpI GC'TnA_GC
Cuts at: 0 998 1410
Size: 998 412
Bme1580I G_kGCm'C
Cuts at: 0 30 678 1410
Size: 30 648 732
Fragments arranged by size:
732 648 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 1410
Size: 19 1391
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 425 579 1410
Size: 425 154 831
Fragments arranged by size:
831 425 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 444 654 700 707 955 1057 1192
Size: 239 205 210 46 7 248 102 135
Cuts at: 1192 1354 1410
Size: 162 56
Fragments arranged by size:
248 239 210 205 162 135 102 56
46 7
Bpu10I CC'TnA_GC
Cuts at: 0 1135 1410
Size: 1135 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 1131 1410
Size: 1131 279
BsaAI yAC'GTr
Cuts at: 0 186 1410
Size: 186 1224
BsaHI Gr'CG_yC
Cuts at: 0 180 834 1410
Size: 180 654 576
Fragments arranged by size:
654 576 180
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 456 474 889 1410
Size: 340 116 18 415 521
Fragments arranged by size:
521 415 340 116 18
BseYI C'CCAG_C
Cuts at: 0 39 51 113 238 1410
Size: 39 12 62 125 1172
Fragments arranged by size:
1172 125 62 39 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1410
Size: 727 683
BsiHKAI G_wGCw'C
Cuts at: 0 869 1410
Size: 869 541
Bsp1286I G_dGCh'C
Cuts at: 0 18 30 678 869 1410
Size: 18 12 648 191 541
Fragments arranged by size:
648 541 191 18 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1100 1410
Size: 1100 310
BsrBI CCG'CTC
Cuts at: 0 558 1410
Size: 558 852
BsrDI GCAATG_nn'
Cuts at: 0 1064 1410
Size: 1064 346
BsrFI r'CCGG_y
Cuts at: 0 64 815 1410
Size: 64 751 595
Fragments arranged by size:
751 595 64
BstAPI GCAn_nnn'nTGC
Cuts at: 0 928 1115 1410
Size: 928 187 295
Fragments arranged by size:
928 295 187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 121 150 303 678 1410
Size: 121 29 153 375 732
Fragments arranged by size:
732 375 153 121 29
BstYI r'GATC_y
Cuts at: 0 171 1410
Size: 171 1239
Bsu36I CC'TnA_GG
Cuts at: 0 696 1410
Size: 696 714
BtgI C'CryG_G
Cuts at: 0 247 1088 1410
Size: 247 841 322
Fragments arranged by size:
841 322 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 114 1410
Size: 114 1296
EaeI y'GGCC_r
Cuts at: 0 123 1410
Size: 123 1287
EarI CTCTTCn'nnn_
Cuts at: 0 386 644 1410
Size: 386 258 766
Fragments arranged by size:
766 386 258
EcoICRI GAG'CTC
Cuts at: 0 867 1410
Size: 867 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 239 444 644 654 700 707 955
Size: 152 87 205 200 10 46 7 248
Cuts at: 955 1057 1192 1247 1325 1354 1410
Size: 102 135 55 78 29 56
Fragments arranged by size:
248 205 200 152 135 102 87 78
56 55 46 29 10 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 727 1410
Size: 727 683
EcoO109I rG'GnC_Cy
Cuts at: 0 1377 1410
Size: 1377 33
HaeII r_GCGC'y
Cuts at: 0 310 837 1410
Size: 310 527 573
Fragments arranged by size:
573 527 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 156 188 877 909 1410
Size: 102 32 22 32 689 32 501
Fragments arranged by size:
689 501 102 32 32 32 22
KasI G'GCGC_C
Cuts at: 0 833 1410
Size: 833 577
KpnI G_GTAC'C
Cuts at: 0 59 1410
Size: 59 1351
MslI CAynn'nnrTG
Cuts at: 0 749 1410
Size: 749 661
MspA1I CmG'CkG
Cuts at: 0 783 993 1410
Size: 783 210 417
Fragments arranged by size:
783 417 210
NaeI GCC'GGC
Cuts at: 0 66 817 1410
Size: 66 751 593
Fragments arranged by size:
751 593 66
NarI GG'CG_CC
Cuts at: 0 834 1410
Size: 834 576
NcoI C'CATG_G
Cuts at: 0 1088 1410
Size: 1088 322
NdeI CA'TA_TG
Cuts at: 0 897 1410
Size: 897 513
NgoMIV G'CCGG_C
Cuts at: 0 64 815 1410
Size: 64 751 595
Fragments arranged by size:
751 595 64
NspI r_CATG'y
Cuts at: 0 861 1054 1410
Size: 861 193 356
Fragments arranged by size:
861 356 193
PasI CC'CwG_GG
Cuts at: 0 18 1410
Size: 18 1392
PciI A'CATG_T
Cuts at: 0 857 1410
Size: 857 553
PflMI CCAn_nnn'nTGG
Cuts at: 0 405 1410
Size: 405 1005
PfoI T'CCnGG_A
Cuts at: 0 174 434 1410
Size: 174 260 976
Fragments arranged by size:
976 260 174
PmlI CAC'GTG
Cuts at: 0 186 1410
Size: 186 1224
PpuMI rG'GwC_Cy
Cuts at: 0 1377 1410
Size: 1377 33
PstI C_TGCA'G
Cuts at: 0 728 1410
Size: 728 682
PvuII CAG'CTG
Cuts at: 0 783 993 1410
Size: 783 210 417
Fragments arranged by size:
783 417 210
SacI G_AGCT'C
Cuts at: 0 869 1410
Size: 869 541
SfcI C'TryA_G
Cuts at: 0 724 849 1160 1234 1281 1410
Size: 724 125 311 74 47 129
Fragments arranged by size:
724 311 129 125 74 47
SfoI GGC'GCC
Cuts at: 0 835 1410
Size: 835 575
StyI C'CwwG_G
Cuts at: 0 69 1088 1410
Size: 69 1019 322
Fragments arranged by size:
1019 322 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 10 1410
Size: 10 1400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 170 202 1410
Size: 170 32 1208
Fragments arranged by size:
1208 170 32
XbaI T'CTAG_A
Cuts at: 0 4 1410
Size: 4 1406
XmnI GAAnn'nnTTC
Cuts at: 0 630 1410
Size: 630 780
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlwNI
AvaI BanI BanII BbeI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PstI PvuII SacI
SfcI SfoI StyI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI MscI NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XcmI XhoI XmaI ZraI