Restriction map for
pFN21AE1932
(Linear) (Six-base) MAPSORT of: KIEE1932.seq Check: 8952 from: 1 to: 1410
KIEE1932.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1410
Size: 943 467
AleI CACnn'nnGTG
Cuts at: 0 877 1410
Size: 877 533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 50 1410
Size: 50 1360
AlwNI CAG_nnn'CTG
Cuts at: 0 780 1410
Size: 780 630
ApaI G_GGCC'C
Cuts at: 0 320 1410
Size: 320 1090
ApoI r'AATT_y
Cuts at: 0 115 442 601 1410
Size: 115 327 159 809
Fragments arranged by size:
809 327 159 115
BanII G_rGCy'C
Cuts at: 0 48 320 501 1410
Size: 48 272 181 909
Fragments arranged by size:
909 272 181 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 1287 1410
Size: 1287 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 284 1410
Size: 250 34 1126
Fragments arranged by size:
1126 250 34
BglI GCCn_nnn'nGGC
Cuts at: 0 315 1410
Size: 315 1095
BglII A'GATC_T
Cuts at: 0 890 1410
Size: 890 520
Bme1580I G_kGCm'C
Cuts at: 0 320 1410
Size: 320 1090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 271 1410
Size: 62 209 1139
Fragments arranged by size:
1139 209 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1410
Size: 148 1262
BsaI GGTCTCn'nnnn_
Cuts at: 0 782 930 1410
Size: 782 148 480
Fragments arranged by size:
782 480 148
BsaAI yAC'GTr
Cuts at: 0 766 1410
Size: 766 644
BsaBI GATnn'nnATC
Cuts at: 0 290 1410
Size: 290 1120
BsaWI w'CCGG_w
Cuts at: 0 401 1410
Size: 401 1009
BsiHKAI G_wGCw'C
Cuts at: 0 48 501 1410
Size: 48 453 909
Fragments arranged by size:
909 453 48
BsmI GAATG_Cn'
Cuts at: 0 770 1410
Size: 770 640
Bsp1286I G_dGCh'C
Cuts at: 0 48 320 501 1410
Size: 48 272 181 909
Fragments arranged by size:
909 272 181 48
BspEI T'CCGG_A
Cuts at: 0 401 1410
Size: 401 1009
BssSI C'ACGA_G
Cuts at: 0 652 1410
Size: 652 758
BstXI CCAn_nnnn'nTGG
Cuts at: 0 553 1410
Size: 553 857
BstYI r'GATC_y
Cuts at: 0 562 890 1333 1410
Size: 562 328 443 77
Fragments arranged by size:
562 443 328 77
Bsu36I CC'TnA_GG
Cuts at: 0 669 1410
Size: 669 741
BtgI C'CryG_G
Cuts at: 0 492 878 1410
Size: 492 386 532
Fragments arranged by size:
532 492 386
BtsI GCAGTG_nn'
Cuts at: 0 32 641 1410
Size: 32 609 769
Fragments arranged by size:
769 609 32
DraI TTT'AAA
Cuts at: 0 1041 1410
Size: 1041 369
EaeI y'GGCC_r
Cuts at: 0 660 678 973 1410
Size: 660 18 295 437
Fragments arranged by size:
660 437 295 18
EarI CTCTTCn'nnn_
Cuts at: 0 53 1410
Size: 53 1357
EcoICRI GAG'CTC
Cuts at: 0 46 499 1410
Size: 46 453 911
Fragments arranged by size:
911 453 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 271 943 1410
Size: 62 209 672 467
Fragments arranged by size:
672 467 209 62
EcoO109I rG'GnC_Cy
Cuts at: 0 316 1172 1410
Size: 316 856 238
Fragments arranged by size:
856 316 238
EcoRI G'AATT_C
Cuts at: 0 601 1410
Size: 601 809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1051 1410
Size: 1051 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 1410
Size: 263 32 1115
Fragments arranged by size:
1115 263 32
HincII GTy'rAC
Cuts at: 0 390 729 1410
Size: 390 339 681
Fragments arranged by size:
681 390 339
HindIII A'AGCT_T
Cuts at: 0 407 1410
Size: 407 1003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 1410
Size: 1389 21
MscI TGG'CCA
Cuts at: 0 662 680 975 1410
Size: 662 18 295 435
Fragments arranged by size:
662 435 295 18
MslI CAynn'nnrTG
Cuts at: 0 270 463 478 590 686 877 1410
Size: 270 193 15 112 96 191 533
Fragments arranged by size:
533 270 193 191 112 96 15
MspA1I CmG'CkG
Cuts at: 0 1147 1410
Size: 1147 263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 824 856 1410
Size: 824 32 554
Fragments arranged by size:
824 554 32
PpuMI rG'GwC_Cy
Cuts at: 0 1172 1410
Size: 1172 238
PspOMI G'GGCC_C
Cuts at: 0 316 1410
Size: 316 1094
PstI C_TGCA'G
Cuts at: 0 29 1410
Size: 29 1381
PvuII CAG'CTG
Cuts at: 0 1147 1410
Size: 1147 263
SacI G_AGCT'C
Cuts at: 0 48 501 1410
Size: 48 453 909
Fragments arranged by size:
909 453 48
SapI GCTCTTCn'nnn_
Cuts at: 0 53 1410
Size: 53 1357
SbfI CC_TGCA'GG
Cuts at: 0 29 1410
Size: 29 1381
SfcI C'TryA_G
Cuts at: 0 25 345 1410
Size: 25 320 1065
Fragments arranged by size:
1065 320 25
SmlI C'TyrA_G
Cuts at: 0 127 1410
Size: 127 1283
StyI C'CwwG_G
Cuts at: 0 1266 1329 1410
Size: 1266 63 81
Fragments arranged by size:
1266 81 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 520 1410
Size: 520 890
XmnI GAAnn'nnTTC
Cuts at: 0 409 1410
Size: 409 1001
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApoI BanII BbsI
BcgI BglI BglII Bme1580I BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsiHKAI BsmI Bsp1286I BspEI BssSI BstXI
BstYI Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI
MscI MslI MspA1I PpiI PpuMI PspOMI PstI PvuII
SacI SapI SbfI SfcI SmlI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoNI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI