Restriction map for
pFN21AE1930
(Linear) (Six-base) MAPSORT of: KIEE1930.seq Check: 8922 from: 1 to: 1407
KIEE1930.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AccI GT'mk_AC
Cuts at: 0 716 863 1407
Size: 716 147 544
Fragments arranged by size:
716 544 147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 928 1407
Size: 769 159 479
Fragments arranged by size:
769 479 159
AlwNI CAG_nnn'CTG
Cuts at: 0 892 1407
Size: 892 515
ApaLI G'TGCA_C
Cuts at: 0 18 1407
Size: 18 1389
ApoI r'AATT_y
Cuts at: 0 229 1273 1407
Size: 229 1044 134
Fragments arranged by size:
1044 229 134
AvrII C'CTAG_G
Cuts at: 0 1179 1407
Size: 1179 228
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 338 371 1407
Size: 338 33 1036
Fragments arranged by size:
1036 338 33
BanI G'GyrC_C
Cuts at: 0 1147 1407
Size: 1147 260
BanII G_rGCy'C
Cuts at: 0 264 886 1259 1407
Size: 264 622 373 148
Fragments arranged by size:
622 373 264 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 1363 1407
Size: 496 867 44
Fragments arranged by size:
867 496 44
BbvCI CC'TCA_GC
Cuts at: 0 673 1407
Size: 673 734
BciVI GTATCCnnnnn_n'
Cuts at: 0 318 960 1407
Size: 318 642 447
Fragments arranged by size:
642 447 318
BglI GCCn_nnn'nGGC
Cuts at: 0 1200 1407
Size: 1200 207
Bme1580I G_kGCm'C
Cuts at: 0 22 1407
Size: 22 1385
BmgBI CAC'GTC
Cuts at: 0 57 1407
Size: 57 1350
BmrI ACTGGGnnnn_n'
Cuts at: 0 1111 1407
Size: 1111 296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 678 972 1407
Size: 502 176 294 435
Fragments arranged by size:
502 435 294 176
Bpu10I CC'TnA_GC
Cuts at: 0 475 673 1407
Size: 475 198 734
Fragments arranged by size:
734 475 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1407
Size: 595 812
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 1407
Size: 436 971
BsaBI GATnn'nnATC
Cuts at: 0 941 1407
Size: 941 466
BsaWI w'CCGG_w
Cuts at: 0 1166 1407
Size: 1166 241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 223 475 556 1407
Size: 220 3 252 81 851
Fragments arranged by size:
851 252 220 81 3
BseYI C'CCAG_C
Cuts at: 0 263 885 975 995 1091 1407
Size: 263 622 90 20 96 316
Fragments arranged by size:
622 316 263 96 90 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1407
Size: 1139 268
BsiEI CG_ry'CG
Cuts at: 0 867 1407
Size: 867 540
BsiHKAI G_wGCw'C
Cuts at: 0 15 22 397 1407
Size: 15 7 375 1010
Fragments arranged by size:
1010 375 15 7
BsmI GAATG_Cn'
Cuts at: 0 1176 1407
Size: 1176 231
Bsp1286I G_dGCh'C
Cuts at: 0 15 22 264 397 886 1259 1407
Size: 15 7 242 133 489 373 148
Fragments arranged by size:
489 373 242 148 133 15 7
BspEI T'CCGG_A
Cuts at: 0 1166 1407
Size: 1166 241
BspHI T'CATG_A
Cuts at: 0 791 1298 1407
Size: 791 507 109
Fragments arranged by size:
791 507 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 845 1407
Size: 142 703 562
Fragments arranged by size:
703 562 142
BsrDI GCAATG_nn'
Cuts at: 0 348 1309 1407
Size: 348 961 98
Fragments arranged by size:
961 348 98
BsrFI r'CCGG_y
Cuts at: 0 470 917 1407
Size: 470 447 490
Fragments arranged by size:
490 470 447
BstAPI GCAn_nnn'nTGC
Cuts at: 0 26 1407
Size: 26 1381
Bsu36I CC'TnA_GG
Cuts at: 0 533 651 1407
Size: 533 118 756
Fragments arranged by size:
756 533 118
BtgI C'CryG_G
Cuts at: 0 494 1407
Size: 494 913
BtsI GCAGTG_nn'
Cuts at: 0 69 100 1407
Size: 69 31 1307
Fragments arranged by size:
1307 69 31
DraIII CAC_nnn'GTG
Cuts at: 0 1105 1407
Size: 1105 302
DrdI GACnn_nn'nnGTC
Cuts at: 0 301 1407
Size: 301 1106
EaeI y'GGCC_r
Cuts at: 0 59 75 848 919 1107 1263 1407
Size: 59 16 773 71 188 156 144
Fragments arranged by size:
773 188 156 144 71 59 16
EarI CTCTTCn'nnn_
Cuts at: 0 190 1378 1407
Size: 190 1188 29
Fragments arranged by size:
1188 190 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 904 1294 1407
Size: 904 390 113
Fragments arranged by size:
904 390 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 678 769 928 972 1407
Size: 502 176 91 159 44 435
Fragments arranged by size:
502 435 176 159 91 44
EcoO109I rG'GnC_Cy
Cuts at: 0 1075 1407
Size: 1075 332
EcoRI G'AATT_C
Cuts at: 0 229 1407
Size: 229 1178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 1407
Size: 204 1203
HaeII r_GCGC'y
Cuts at: 0 531 1407
Size: 531 876
HincII GTy'rAC
Cuts at: 0 864 1407
Size: 864 543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 109 1407
Size: 56 53 1298
Fragments arranged by size:
1298 56 53
MscI TGG'CCA
Cuts at: 0 850 1109 1407
Size: 850 259 298
Fragments arranged by size:
850 298 259
MslI CAynn'nnrTG
Cuts at: 0 562 1297 1407
Size: 562 735 110
Fragments arranged by size:
735 562 110
MspA1I CmG'CkG
Cuts at: 0 577 588 1407
Size: 577 11 819
Fragments arranged by size:
819 577 11
NaeI GCC'GGC
Cuts at: 0 472 919 1407
Size: 472 447 488
Fragments arranged by size:
488 472 447
NcoI C'CATG_G
Cuts at: 0 494 1407
Size: 494 913
NgoMIV G'CCGG_C
Cuts at: 0 470 917 1407
Size: 470 447 490
Fragments arranged by size:
490 470 447
PstI C_TGCA'G
Cuts at: 0 365 824 1407
Size: 365 459 583
Fragments arranged by size:
583 459 365
PvuII CAG'CTG
Cuts at: 0 588 1407
Size: 588 819
SalI G'TCGA_C
Cuts at: 0 862 1407
Size: 862 545
SapI GCTCTTCn'nnn_
Cuts at: 0 190 1407
Size: 190 1217
ScaI AGT'ACT
Cuts at: 0 421 1407
Size: 421 986
SfcI C'TryA_G
Cuts at: 0 361 820 1407
Size: 361 459 587
Fragments arranged by size:
587 459 361
SmlI C'TyrA_G
Cuts at: 0 610 1407
Size: 610 797
StuI AGG'CCT
Cuts at: 0 370 649 1407
Size: 370 279 758
Fragments arranged by size:
758 370 279
StyI C'CwwG_G
Cuts at: 0 371 494 1179 1331 1407
Size: 371 123 685 152 76
Fragments arranged by size:
685 371 152 123 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 550 1093 1407
Size: 550 543 314
Fragments arranged by size:
550 543 314
TatI w'GTAC_w
Cuts at: 0 419 596 1407
Size: 419 177 811
Fragments arranged by size:
811 419 177
Tth111I GACn'n_nGTC
Cuts at: 0 253 1294 1407
Size: 253 1041 113
Fragments arranged by size:
1041 253 113
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI AvrII BaeI BanI
BanII BbsI BbvCI BciVI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BstAPI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PstI PvuII SalI SapI ScaI SfcI SmlI StuI
StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI BamHI BbeI BcgI BclI BfrBI BglII BlpI
BmtI BplI BsaAI BsaHI BsaXI BsiWI BsmBI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraI EagI EcoICRI EcoNI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI