Restriction map for
pFN21AE1926
(Linear) (Six-base) MAPSORT of: KIEE1926.seq Check: 1694 from: 1 to: 1392
KIEE1926.orf
With 182 enzymes: SgfI *
July 3, 2012 17:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1065 1392
Size: 908 157 327
Fragments arranged by size:
908 327 157
AleI CACnn'nnGTG
Cuts at: 0 898 1392
Size: 898 494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 1392
Size: 916 476
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 197 229 850 882 1392
Size: 197 32 621 32 510
Fragments arranged by size:
621 510 197 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 291 399 612 1392
Size: 291 108 213 780
Fragments arranged by size:
780 291 213 108
BanI G'GyrC_C
Cuts at: 0 373 892 1392
Size: 373 519 500
Fragments arranged by size:
519 500 373
BanII G_rGCy'C
Cuts at: 0 106 202 725 1392
Size: 106 96 523 667
Fragments arranged by size:
667 523 106 96
BclI T'GATC_A
Cuts at: 0 444 1392
Size: 444 948
BfrBI ATG'CAT
Cuts at: 0 1195 1392
Size: 1195 197
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 619 1195 1392
Size: 619 576 197
Fragments arranged by size:
619 576 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 589 1392
Size: 376 213 803
Fragments arranged by size:
803 376 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1234 1392
Size: 925 309 158
Fragments arranged by size:
925 309 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 556 1392
Size: 556 836
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 1392
Size: 490 30 872
Fragments arranged by size:
872 490 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1045 1392
Size: 1045 347
BseYI C'CCAG_C
Cuts at: 0 369 1392
Size: 369 1023
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 1392
Size: 102 1290
BsiHKAI G_wGCw'C
Cuts at: 0 725 1392
Size: 725 667
BsmI GAATG_Cn'
Cuts at: 0 1195 1392
Size: 1195 197
Bsp1286I G_dGCh'C
Cuts at: 0 106 202 725 1392
Size: 106 96 523 667
Fragments arranged by size:
667 523 106 96
BsrBI CCG'CTC
Cuts at: 0 167 646 1392
Size: 167 479 746
Fragments arranged by size:
746 479 167
BsrFI r'CCGG_y
Cuts at: 0 230 1392
Size: 230 1162
BstYI r'GATC_y
Cuts at: 0 212 449 1392
Size: 212 237 943
Fragments arranged by size:
943 237 212
BtgI C'CryG_G
Cuts at: 0 130 347 935 1392
Size: 130 217 588 457
Fragments arranged by size:
588 457 217 130
BtsI GCAGTG_nn'
Cuts at: 0 1239 1392
Size: 1239 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 336 371 1392
Size: 336 35 1021
Fragments arranged by size:
1021 336 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1183 1392
Size: 1183 209
EaeI y'GGCC_r
Cuts at: 0 647 971 1269 1392
Size: 647 324 298 123
Fragments arranged by size:
647 324 298 123
EarI CTCTTCn'nnn_
Cuts at: 0 958 1392
Size: 958 434
EcoICRI GAG'CTC
Cuts at: 0 723 1392
Size: 723 669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 589 908 1065 1392
Size: 376 213 319 157 327
Fragments arranged by size:
376 327 319 213 157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1392
Size: 973 419
EcoO109I rG'GnC_Cy
Cuts at: 0 762 1392
Size: 762 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 412 424 444 456 635 667
Size: 184 32 196 12 20 12 179 32
Cuts at: 667 814 846 1392
Size: 147 32 546
Fragments arranged by size:
546 196 184 179 147 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 0 744 1392
Size: 744 648
HpaI GTT'AAC
Cuts at: 0 744 1392
Size: 744 648
MscI TGG'CCA
Cuts at: 0 973 1392
Size: 973 419
MslI CAynn'nnrTG
Cuts at: 0 142 898 934 1392
Size: 142 756 36 458
Fragments arranged by size:
756 458 142 36
MspA1I CmG'CkG
Cuts at: 0 132 1098 1243 1255 1392
Size: 132 966 145 12 137
Fragments arranged by size:
966 145 137 132 12
NcoI C'CATG_G
Cuts at: 0 935 1392
Size: 935 457
NsiI A_TGCA'T
Cuts at: 0 1197 1392
Size: 1197 195
NspI r_CATG'y
Cuts at: 0 1056 1392
Size: 1056 336
PflMI CCAn_nnn'nTGG
Cuts at: 0 612 1392
Size: 612 780
PfoI T'CCnGG_A
Cuts at: 0 215 1392
Size: 215 1177
PsiI TTA'TAA
Cuts at: 0 1330 1392
Size: 1330 62
PvuII CAG'CTG
Cuts at: 0 1098 1243 1392
Size: 1098 145 149
Fragments arranged by size:
1098 149 145
SacI G_AGCT'C
Cuts at: 0 725 1392
Size: 725 667
SacII CC_GC'GG
Cuts at: 0 133 1392
Size: 133 1259
SapI GCTCTTCn'nnn_
Cuts at: 0 958 1392
Size: 958 434
SmlI C'TyrA_G
Cuts at: 0 904 1213 1392
Size: 904 309 179
Fragments arranged by size:
904 309 179
StuI AGG'CCT
Cuts at: 0 1314 1392
Size: 1314 78
StyI C'CwwG_G
Cuts at: 0 251 935 974 1392
Size: 251 684 39 418
Fragments arranged by size:
684 418 251 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 361 651 691 910 1392
Size: 361 290 40 219 482
Fragments arranged by size:
482 361 290 219 40
Enzymes that do cut:
AcuI AleI AlfI AloI AlwNI BanI BanII BclI
BfrBI BplI BpmI BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BstYI BtgI
BtsI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I HincII HpaI MscI MslI MspA1I NcoI
NsiI NspI PflMI PfoI PsiI PvuII SacI SacII
SapI SmlI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI