Restriction map for
pFN21AE1925
(Linear) (Six-base) MAPSORT of: KIEE1925.seq Check: 3573 from: 1 to: 1386
KIEE1925.orf
With 182 enzymes: SgfI *
March 22, 2012 15:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 1386
Size: 351 1035
AccI GT'mk_AC
Cuts at: 0 686 1386
Size: 686 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1182 1362 1386
Size: 502 680 180 24
Fragments arranged by size:
680 502 180 24
AfeI AGC'GCT
Cuts at: 0 1135 1386
Size: 1135 251
AhdI GACnn_n'nnGTC
Cuts at: 0 765 1386
Size: 765 621
AleI CACnn'nnGTG
Cuts at: 0 196 514 1144 1386
Size: 196 318 630 242
Fragments arranged by size:
630 318 242 196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 443 1386
Size: 443 943
ApaI G_GGCC'C
Cuts at: 0 52 757 1022 1386
Size: 52 705 265 364
Fragments arranged by size:
705 364 265 52
AvaI C'yCGr_G
Cuts at: 0 102 1014 1386
Size: 102 912 372
Fragments arranged by size:
912 372 102
BanI G'GyrC_C
Cuts at: 0 586 887 1079 1239 1386
Size: 586 301 192 160 147
Fragments arranged by size:
586 301 192 160 147
BanII G_rGCy'C
Cuts at: 0 52 338 757 1022 1386
Size: 52 286 419 265 364
Fragments arranged by size:
419 364 286 265 52
BbeI G_GCGC'C
Cuts at: 0 590 891 1083 1386
Size: 590 301 192 303
Fragments arranged by size:
590 303 301 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 1308 1386
Size: 180 1128 78
Fragments arranged by size:
1128 180 78
BglI GCCn_nnn'nGGC
Cuts at: 0 106 1214 1386
Size: 106 1108 172
Fragments arranged by size:
1108 172 106
BlpI GC'TnA_GC
Cuts at: 0 730 1386
Size: 730 656
Bme1580I G_kGCm'C
Cuts at: 0 52 757 824 996 1022 1244 1386
Size: 52 705 67 172 26 222 142
Fragments arranged by size:
705 222 172 142 67 52 26
BmgBI CAC'GTC
Cuts at: 0 846 1386
Size: 846 540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1386
Size: 990 396
BsaI GGTCTCn'nnnn_
Cuts at: 0 567 613 1386
Size: 567 46 773
Fragments arranged by size:
773 567 46
BsaHI Gr'CG_yC
Cuts at: 0 4 587 888 1080 1386
Size: 4 583 301 192 306
Fragments arranged by size:
583 306 301 192 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1380 1386
Size: 1380 6
BseYI C'CCAG_C
Cuts at: 0 789 1386
Size: 789 597
BsiEI CG_ry'CG
Cuts at: 0 287 416 539 875 1386
Size: 287 129 123 336 511
Fragments arranged by size:
511 336 287 129 123
BsiHKAI G_wGCw'C
Cuts at: 0 338 1178 1386
Size: 338 840 208
Fragments arranged by size:
840 338 208
Bsp1286I G_dGCh'C
Cuts at: 0 52 338 757 824 996 1022 1178 1244
Size: 52 286 419 67 172 26 156 66
Cuts at: 1244 1386
Size: 142
Fragments arranged by size:
419 286 172 156 142 67 66 52
26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 1386
Size: 351 1035
BsrBI CCG'CTC
Cuts at: 0 62 925 1251 1386
Size: 62 863 326 135
Fragments arranged by size:
863 326 135 62
BsrFI r'CCGG_y
Cuts at: 0 1076 1386
Size: 1076 310
BssHII G'CGCG_C
Cuts at: 0 246 248 691 741 827 1038 1087 1123
Size: 246 2 443 50 86 211 49 36
Cuts at: 1123 1386
Size: 263
Fragments arranged by size:
443 263 246 211 86 50 49 36
2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1100 1386
Size: 1100 286
BstXI CCAn_nnnn'nTGG
Cuts at: 0 438 516 1386
Size: 438 78 870
Fragments arranged by size:
870 438 78
BtgI C'CryG_G
Cuts at: 0 167 194 749 1386
Size: 167 27 555 637
Fragments arranged by size:
637 555 167 27
BtsI GCAGTG_nn'
Cuts at: 0 607 822 1386
Size: 607 215 564
Fragments arranged by size:
607 564 215
EaeI y'GGCC_r
Cuts at: 0 129 284 299 326 518 536 707 872
Size: 129 155 15 27 192 18 171 165
Cuts at: 872 1026 1386
Size: 154 360
Fragments arranged by size:
360 192 171 165 155 154 129 27
18 15
EagI C'GGCC_G
Cuts at: 0 284 536 872 1386
Size: 284 252 336 514
Fragments arranged by size:
514 336 284 252
EarI CTCTTCn'nnn_
Cuts at: 0 268 1258 1360 1386
Size: 268 990 102 26
Fragments arranged by size:
990 268 102 26
EcoICRI GAG'CTC
Cuts at: 0 336 1386
Size: 336 1050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 990 1182 1362 1386
Size: 502 488 192 180 24
Fragments arranged by size:
502 488 192 180 24
EcoO109I rG'GnC_Cy
Cuts at: 0 49 754 1010 1018 1386
Size: 49 705 256 8 368
Fragments arranged by size:
705 368 256 49 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1281 1386
Size: 1281 105
FspI TGC'GCA
Cuts at: 0 718 1386
Size: 718 668
HaeII r_GCGC'y
Cuts at: 0 16 28 244 388 590 891 1083 1137
Size: 16 12 216 144 202 301 192 54
Cuts at: 1137 1386
Size: 249
Fragments arranged by size:
301 249 216 202 192 144 54 16
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 751 783 1386
Size: 405 32 314 32 603
Fragments arranged by size:
603 405 314 32 32
KasI G'GCGC_C
Cuts at: 0 586 887 1079 1386
Size: 586 301 192 307
Fragments arranged by size:
586 307 301 192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1383 1386
Size: 1383 3
MscI TGG'CCA
Cuts at: 0 301 328 709 1386
Size: 301 27 381 677
Fragments arranged by size:
677 381 301 27
MslI CAynn'nnrTG
Cuts at: 0 196 295 425 514 1144 1165 1386
Size: 196 99 130 89 630 21 221
Fragments arranged by size:
630 221 196 130 99 89 21
MspA1I CmG'CkG
Cuts at: 0 43 83 558 871 1386
Size: 43 40 475 313 515
Fragments arranged by size:
515 475 313 43 40
NaeI GCC'GGC
Cuts at: 0 1078 1386
Size: 1078 308
NarI GG'CG_CC
Cuts at: 0 587 888 1080 1386
Size: 587 301 192 306
Fragments arranged by size:
587 306 301 192
NgoMIV G'CCGG_C
Cuts at: 0 1076 1386
Size: 1076 310
PpuMI rG'GwC_Cy
Cuts at: 0 1010 1386
Size: 1010 376
PspOMI G'GGCC_C
Cuts at: 0 48 753 1018 1386
Size: 48 705 265 368
Fragments arranged by size:
705 368 265 48
PvuI CG_AT'CG
Cuts at: 0 416 1386
Size: 416 970
SacI G_AGCT'C
Cuts at: 0 338 1386
Size: 338 1048
SapI GCTCTTCn'nnn_
Cuts at: 0 1258 1386
Size: 1258 128
ScaI AGT'ACT
Cuts at: 0 136 1386
Size: 136 1250
SexAI A'CCwGG_T
Cuts at: 0 622 1386
Size: 622 764
SfcI C'TryA_G
Cuts at: 0 1203 1386
Size: 1203 183
SfoI GGC'GCC
Cuts at: 0 588 889 1081 1386
Size: 588 301 192 305
Fragments arranged by size:
588 305 301 192
SgrAI Cr'CCGG_yG
Cuts at: 0 1076 1386
Size: 1076 310
SmaI CCC'GGG
Cuts at: 0 1016 1386
Size: 1016 370
StuI AGG'CCT
Cuts at: 0 1231 1386
Size: 1231 155
TatI w'GTAC_w
Cuts at: 0 134 473 1386
Size: 134 339 913
Fragments arranged by size:
913 339 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 1386
Size: 336 1050
XmaI C'CCGG_G
Cuts at: 0 1014 1386
Size: 1014 372
XmnI GAAnn'nnTTC
Cuts at: 0 262 1135 1386
Size: 262 873 251
Fragments arranged by size:
873 262 251
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI ApaI
AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BpmI BsaI BsaHI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV PpuMI PspOMI PvuI SacI SapI
ScaI SexAI SfcI SfoI SgrAI SmaI StuI TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AloI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuII RsrII SacII SalI SanDI
SbfI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TstI Tth111I XbaI XhoI
ZraI