Restriction map for
pFN21AE1924
(Linear) (Six-base) MAPSORT of: KIEE1924.seq Check: 4248 from: 1 to: 1383
KIEE1924.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 364 962 1153 1383
Size: 364 598 191 230
Fragments arranged by size:
598 364 230 191
AccI GT'mk_AC
Cuts at: 0 816 1383
Size: 816 567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1383
Size: 773 610
AlwNI CAG_nnn'CTG
Cuts at: 0 460 954 1383
Size: 460 494 429
Fragments arranged by size:
494 460 429
ApaI G_GGCC'C
Cuts at: 0 739 940 1383
Size: 739 201 443
Fragments arranged by size:
739 443 201
AvaI C'yCGr_G
Cuts at: 0 106 271 332 432 495 923 940 1082
Size: 106 165 61 100 63 428 17 142
Cuts at: 1082 1383
Size: 301
Fragments arranged by size:
428 301 165 142 106 100 63 61
17
BanI G'GyrC_C
Cuts at: 0 3 398 897 1023 1047 1383
Size: 3 395 499 126 24 336
Fragments arranged by size:
499 395 336 126 24 3
BanII G_rGCy'C
Cuts at: 0 47 107 739 940 976 1000 1102 1383
Size: 47 60 632 201 36 24 102 281
Fragments arranged by size:
632 281 201 102 60 47 36 24
BbeI G_GCGC'C
Cuts at: 0 7 1027 1383
Size: 7 1020 356
Fragments arranged by size:
1020 356 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 186 1383
Size: 186 1197
BclI T'GATC_A
Cuts at: 0 959 1383
Size: 959 424
BglI GCCn_nnn'nGGC
Cuts at: 0 19 28 79 434 634 919 1069 1078
Size: 19 9 51 355 200 285 150 9
Cuts at: 1078 1383
Size: 305
Fragments arranged by size:
355 305 285 200 150 51 19 9
9
BglII A'GATC_T
Cuts at: 0 548 1383
Size: 548 835
BlpI GC'TnA_GC
Cuts at: 0 382 1383
Size: 382 1001
Bme1580I G_kGCm'C
Cuts at: 0 495 739 940 1383
Size: 495 244 201 443
Fragments arranged by size:
495 443 244 201
BmgBI CAC'GTC
Cuts at: 0 474 602 608 1383
Size: 474 128 6 775
Fragments arranged by size:
775 474 128 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 273 1373 1383
Size: 273 1100 10
Fragments arranged by size:
1100 273 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 674 960 1012 1321 1383
Size: 61 613 286 52 309 62
Fragments arranged by size:
613 309 286 62 61 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 305 1383
Size: 300 5 1078
Fragments arranged by size:
1078 300 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 1306 1383
Size: 1306 77
BsaHI Gr'CG_yC
Cuts at: 0 4 1024 1306 1383
Size: 4 1020 282 77
Fragments arranged by size:
1020 282 77 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1182 1383
Size: 1182 201
BseYI C'CCAG_C
Cuts at: 0 251 650 697 739 747 1320 1366 1383
Size: 251 399 47 42 8 573 46 17
Fragments arranged by size:
573 399 251 47 46 42 17 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 336 582 1383
Size: 312 24 246 801
Fragments arranged by size:
801 312 246 24
BsiHKAI G_wGCw'C
Cuts at: 0 47 115 763 976 1000 1102 1192 1330
Size: 47 68 648 213 24 102 90 138
Cuts at: 1330 1383
Size: 53
Fragments arranged by size:
648 213 138 102 90 68 53 47
24
BsmI GAATG_Cn'
Cuts at: 0 582 1383
Size: 582 801
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1297 1383
Size: 1297 86
Bsp1286I G_dGCh'C
Cuts at: 0 47 107 115 495 739 763 940 976
Size: 47 60 8 380 244 24 177 36
Cuts at: 976 1000 1102 1192 1330 1383
Size: 24 102 90 138 53
Fragments arranged by size:
380 244 177 138 102 90 60 53
47 36 24 24 8
BspHI T'CATG_A
Cuts at: 0 848 1383
Size: 848 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 364 962 1153 1248 1383
Size: 364 598 191 95 135
Fragments arranged by size:
598 364 191 135 95
BsrFI r'CCGG_y
Cuts at: 0 119 401 1383
Size: 119 282 982
Fragments arranged by size:
982 282 119
BssHII G'CGCG_C
Cuts at: 0 377 410 1383
Size: 377 33 973
Fragments arranged by size:
973 377 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 66 167 954 1383
Size: 66 101 787 429
Fragments arranged by size:
787 429 101 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1011 1383
Size: 1011 372
BstYI r'GATC_y
Cuts at: 0 548 1383
Size: 548 835
BtgI C'CryG_G
Cuts at: 0 524 1122 1383
Size: 524 598 261
Fragments arranged by size:
598 524 261
BtsI GCAGTG_nn'
Cuts at: 0 701 1383
Size: 701 682
DraIII CAC_nnn'GTG
Cuts at: 0 221 1383
Size: 221 1162
DrdI GACnn_nn'nnGTC
Cuts at: 0 1258 1383
Size: 1258 125
EaeI y'GGCC_r
Cuts at: 0 29 446 563 1001 1119 1383
Size: 29 417 117 438 118 264
Fragments arranged by size:
438 417 264 118 117 29
EarI CTCTTCn'nnn_
Cuts at: 0 782 1328 1383
Size: 782 546 55
Fragments arranged by size:
782 546 55
EcoICRI GAG'CTC
Cuts at: 0 45 974 998 1100 1383
Size: 45 929 24 102 283
Fragments arranged by size:
929 283 102 45 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 674 773 960 1012 1321 1383
Size: 61 613 99 187 52 309 62
Fragments arranged by size:
613 309 187 99 62 61 52
EcoO109I rG'GnC_Cy
Cuts at: 0 539 736 936 937 1127 1260 1383
Size: 539 197 200 1 190 133 123
Fragments arranged by size:
539 200 197 190 133 123 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 888 1383
Size: 888 495
FspI TGC'GCA
Cuts at: 0 421 1383
Size: 421 962
HaeII r_GCGC'y
Cuts at: 0 7 970 1027 1383
Size: 7 963 57 356
Fragments arranged by size:
963 356 57 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 1165 1197 1383
Size: 29 32 1104 32 186
Fragments arranged by size:
1104 186 32 32 29
KasI G'GCGC_C
Cuts at: 0 3 1023 1383
Size: 3 1020 360
Fragments arranged by size:
1020 360 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 533 765 992 1383
Size: 15 518 232 227 391
Fragments arranged by size:
518 391 232 227 15
MscI TGG'CCA
Cuts at: 0 448 565 1121 1383
Size: 448 117 556 262
Fragments arranged by size:
556 448 262 117
MspA1I CmG'CkG
Cuts at: 0 296 417 751 1299 1383
Size: 296 121 334 548 84
Fragments arranged by size:
548 334 296 121 84
NaeI GCC'GGC
Cuts at: 0 121 403 1383
Size: 121 282 980
Fragments arranged by size:
980 282 121
NarI GG'CG_CC
Cuts at: 0 4 1024 1383
Size: 4 1020 359
Fragments arranged by size:
1020 359 4
NcoI C'CATG_G
Cuts at: 0 1122 1383
Size: 1122 261
NgoMIV G'CCGG_C
Cuts at: 0 119 401 1383
Size: 119 282 982
Fragments arranged by size:
982 282 119
NspI r_CATG'y
Cuts at: 0 119 187 1383
Size: 119 68 1196
Fragments arranged by size:
1196 119 68
PasI CC'CwG_GG
Cuts at: 0 571 631 1383
Size: 571 60 752
Fragments arranged by size:
752 571 60
PpuMI rG'GwC_Cy
Cuts at: 0 1127 1260 1383
Size: 1127 133 123
Fragments arranged by size:
1127 133 123
PshAI GACnn'nnGTC
Cuts at: 0 1132 1383
Size: 1132 251
PspOMI G'GGCC_C
Cuts at: 0 735 936 1383
Size: 735 201 447
Fragments arranged by size:
735 447 201
PstI C_TGCA'G
Cuts at: 0 163 361 884 1383
Size: 163 198 523 499
Fragments arranged by size:
523 499 198 163
PvuII CAG'CTG
Cuts at: 0 751 1299 1383
Size: 751 548 84
Fragments arranged by size:
751 548 84
SacI G_AGCT'C
Cuts at: 0 47 976 1000 1102 1383
Size: 47 929 24 102 281
Fragments arranged by size:
929 281 102 47 24
SanDI GG'GwC_CC
Cuts at: 0 1127 1383
Size: 1127 256
SapI GCTCTTCn'nnn_
Cuts at: 0 782 1383
Size: 782 601
SbfI CC_TGCA'GG
Cuts at: 0 361 1383
Size: 361 1022
SexAI A'CCwGG_T
Cuts at: 0 308 1383
Size: 308 1075
SfcI C'TryA_G
Cuts at: 0 159 297 357 880 1161 1383
Size: 159 138 60 523 281 222
Fragments arranged by size:
523 281 222 159 138 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 28 1069 1078 1383
Size: 28 1041 9 305
Fragments arranged by size:
1041 305 28 9
SfoI GGC'GCC
Cuts at: 0 5 1025 1383
Size: 5 1020 358
Fragments arranged by size:
1020 358 5
SmaI CCC'GGG
Cuts at: 0 108 434 497 925 1084 1383
Size: 108 326 63 428 159 299
Fragments arranged by size:
428 326 299 159 108 63
SmlI C'TyrA_G
Cuts at: 0 279 320 1383
Size: 279 41 1063
Fragments arranged by size:
1063 279 41
SrfI GCCC'GGGC
Cuts at: 0 434 1084 1383
Size: 434 650 299
Fragments arranged by size:
650 434 299
StyI C'CwwG_G
Cuts at: 0 809 893 1122 1271 1350 1383
Size: 809 84 229 149 79 33
Fragments arranged by size:
809 229 149 84 79 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 787 1280 1383
Size: 265 522 493 103
Fragments arranged by size:
522 493 265 103
TatI w'GTAC_w
Cuts at: 0 639 1383
Size: 639 744
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 779 811 1383
Size: 205 32 542 32 572
Fragments arranged by size:
572 542 205 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 1209 1383
Size: 621 588 174
Fragments arranged by size:
621 588 174
XmaI C'CCGG_G
Cuts at: 0 106 432 495 923 1082 1383
Size: 106 326 63 428 159 301
Fragments arranged by size:
428 326 301 159 106 63
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BssHII
BstAPI BstXI BstYI BtgI BtsI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I
KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PpuMI PshAI PspOMI PstI PvuII SacI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI
SmlI SrfI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BspEI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoNI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI ScaI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I
XbaI XhoI XmnI ZraI