(Linear) (Six-base) MAPSORT of: KIEE1923.seq  Check: 9149  from: 1  to: 1380



KIEE1923.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:50  ..

AccI GT'mk_AC

Cuts at:      0     815    1380 
   Size:        815     565
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     670    1380 
   Size:        136     534     710

  Fragments arranged by size: 

                 710     534     136
 
ApoI r'AATT_y

Cuts at:      0     131     392     638     934    1380 
   Size:        131     261     246     296     446

  Fragments arranged by size: 

                 446     296     261     246     131
 
AseI AT'TA_AT

Cuts at:      0     203     850    1380 
   Size:        203     647     530

  Fragments arranged by size: 

                 647     530     203
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     554    1013    1380 
   Size:        554     459     367

  Fragments arranged by size: 

                 554     459     367
 
BclI T'GATC_A

Cuts at:      0      57     234    1380 
   Size:         57     177    1146

  Fragments arranged by size: 

                1146     177      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      74     449     520    1380 
   Size:         33      41     375      71     860

  Fragments arranged by size: 

                 860     375      71      41      33
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785    1380 
   Size:        785     595
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1296    1380 
   Size:       1296      84
 
BseYI C'CCAG_C

Cuts at:      0     790    1380 
   Size:        790     590
 
BsiHKAI G_wGCw'C

Cuts at:      0     666    1380 
   Size:        666     714
 
BsmI GAATG_Cn'

Cuts at:      0     722    1380 
   Size:        722     658
 
Bsp1286I G_dGCh'C

Cuts at:      0     666    1380 
   Size:        666     714
 
BsrDI GCAATG_nn'

Cuts at:      0    1107    1380 
   Size:       1107     273
 
BtgI C'CryG_G

Cuts at:      0     542     841    1380 
   Size:        542     299     539

  Fragments arranged by size: 

                 542     539     299
 
EarI CTCTTCn'nnn_

Cuts at:      0     326     419    1281    1380 
   Size:        326      93     862      99

  Fragments arranged by size: 

                 862     326      99      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      74     136     449     520     670    1380 
   Size:         33      41      62     313      71     150     710

  Fragments arranged by size: 

                 710     313     150      71      62      41      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      70      85      87     102     117     814     846 
   Size:         55      15      15       2      15      15     697      32

Cuts at:    846    1380 
   Size:        534

  Fragments arranged by size: 

                 697     534      55      32      15      15      15      15
                   2
 
HindIII A'AGCT_T

Cuts at:      0    1371    1380 
   Size:       1371       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     983    1380 
   Size:        983     397
 
MslI CAynn'nnrTG

Cuts at:      0     767    1380 
   Size:        767     613
 
NcoI C'CATG_G

Cuts at:      0     841    1380 
   Size:        841     539
 
NspI r_CATG'y

Cuts at:      0     777    1380 
   Size:        777     603
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     543    1380 
   Size:        543     837
 
PfoI T'CCnGG_A

Cuts at:      0    1118    1380 
   Size:       1118     262
 
PsiI TTA'TAA

Cuts at:      0     749    1380 
   Size:        749     631
 
SapI GCTCTTCn'nnn_

Cuts at:      0     419    1380 
   Size:        419     961
 
SmlI C'TyrA_G

Cuts at:      0     800    1380 
   Size:        800     580
 
SpeI A'CTAG_T

Cuts at:      0     616    1380 
   Size:        616     764
 
SspI AAT'ATT

Cuts at:      0     418    1039    1380 
   Size:        418     621     341

  Fragments arranged by size: 

                 621     418     341
 
StyI C'CwwG_G

Cuts at:      0     841    1380 
   Size:        841     539
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     488    1380 
   Size:        488     892
 
Tth111I GACn'n_nGTC

Cuts at:      0     164     178    1380 
   Size:        164      14    1202

  Fragments arranged by size: 

                1202     164      14
 
XmnI GAAnn'nnTTC

Cuts at:      0     392    1380 
   Size:        392     988
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AseI     BbsI     BclI     BpmI    BpuEI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI     BtgI     EarI
  Eco57MI    Hin4I  HindIII     MmeI     MslI     NcoI     NspI    PflMI
     PfoI     PsiI     SapI     SmlI     SpeI     SspI     StyI    TaqII
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     StuI     SwaI     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI