Restriction map for
pFN21AE1921
(Linear) (Six-base) MAPSORT of: KIEE1921.seq Check: 1836 from: 1 to: 1371
KIEE1921.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 220 1229 1371
Size: 220 1009 142
Fragments arranged by size:
1009 220 142
Acc65I G'GTAC_C
Cuts at: 0 792 1371
Size: 792 579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1118 1371
Size: 943 175 253
Fragments arranged by size:
943 253 175
AleI CACnn'nnGTG
Cuts at: 0 811 879 1371
Size: 811 68 492
Fragments arranged by size:
811 492 68
AlwNI CAG_nnn'CTG
Cuts at: 0 150 966 1371
Size: 150 816 405
Fragments arranged by size:
816 405 150
ApaI G_GGCC'C
Cuts at: 0 66 898 1371
Size: 66 832 473
Fragments arranged by size:
832 473 66
AvaI C'yCGr_G
Cuts at: 0 230 1371
Size: 230 1141
BamHI G'GATC_C
Cuts at: 0 830 1371
Size: 830 541
BanI G'GyrC_C
Cuts at: 0 49 792 1217 1256 1279 1327 1371
Size: 49 743 425 39 23 48 44
Fragments arranged by size:
743 425 49 48 44 39 23
BanII G_rGCy'C
Cuts at: 0 66 634 898 1121 1371
Size: 66 568 264 223 250
Fragments arranged by size:
568 264 250 223 66
BbeI G_GCGC'C
Cuts at: 0 53 1283 1371
Size: 53 1230 88
Fragments arranged by size:
1230 88 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 433 567 1371
Size: 433 134 804
Fragments arranged by size:
804 433 134
BbvCI CC'TCA_GC
Cuts at: 0 1195 1371
Size: 1195 176
Bme1580I G_kGCm'C
Cuts at: 0 66 898 1332 1371
Size: 66 832 434 39
Fragments arranged by size:
832 434 66 39
BmtI G_CTAG'C
Cuts at: 0 1018 1371
Size: 1018 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 187 358 1326 1371
Size: 172 15 171 968 45
Fragments arranged by size:
968 172 171 45 15
Bpu10I CC'TnA_GC
Cuts at: 0 490 1195 1371
Size: 490 705 176
Fragments arranged by size:
705 490 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 993 1371
Size: 993 378
BsaAI yAC'GTr
Cuts at: 0 1286 1371
Size: 1286 85
BsaHI Gr'CG_yC
Cuts at: 0 50 1229 1280 1371
Size: 50 1179 51 91
Fragments arranged by size:
1179 91 51 50
BsaWI w'CCGG_w
Cuts at: 0 1080 1371
Size: 1080 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 607 637 1371
Size: 607 30 734
Fragments arranged by size:
734 607 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 169 270 861 1329 1371
Size: 118 51 101 591 468 42
Fragments arranged by size:
591 468 118 101 51 42
BseYI C'CCAG_C
Cuts at: 0 161 785 796 854 1371
Size: 161 624 11 58 517
Fragments arranged by size:
624 517 161 58 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 154 297 498 1204 1308 1371
Size: 139 15 143 201 706 104 63
Fragments arranged by size:
706 201 143 139 104 63 15
BsiHKAI G_wGCw'C
Cuts at: 0 625 1121 1371
Size: 625 496 250
Fragments arranged by size:
625 496 250
BsmBI CGTCTCn'nnnn_
Cuts at: 0 741 1371
Size: 741 630
Bsp1286I G_dGCh'C
Cuts at: 0 66 625 634 898 1121 1332 1371
Size: 66 559 9 264 223 211 39
Fragments arranged by size:
559 264 223 211 66 39 9
BspEI T'CCGG_A
Cuts at: 0 1080 1371
Size: 1080 291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 1229 1287 1371
Size: 220 1009 58 84
Fragments arranged by size:
1009 220 84 58
BsrBI CCG'CTC
Cuts at: 0 753 1337 1371
Size: 753 584 34
Fragments arranged by size:
753 584 34
BsrDI GCAATG_nn'
Cuts at: 0 303 356 1371
Size: 303 53 1015
Fragments arranged by size:
1015 303 53
BsrFI r'CCGG_y
Cuts at: 0 72 430 1253 1371
Size: 72 358 823 118
Fragments arranged by size:
823 358 118 72
BsrGI T'GTAC_A
Cuts at: 0 719 1371
Size: 719 652
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1371
Size: 471 900
BstYI r'GATC_y
Cuts at: 0 830 1371
Size: 830 541
Bsu36I CC'TnA_GG
Cuts at: 0 559 1371
Size: 559 812
BtgI C'CryG_G
Cuts at: 0 653 809 1371
Size: 653 156 562
Fragments arranged by size:
653 562 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 807 1371
Size: 807 564
BtsI GCAGTG_nn'
Cuts at: 0 151 510 1371
Size: 151 359 861
Fragments arranged by size:
861 359 151
EaeI y'GGCC_r
Cuts at: 0 81 89 217 1371
Size: 81 8 128 1154
Fragments arranged by size:
1154 128 81 8
EarI CTCTTCn'nnn_
Cuts at: 0 280 547 601 982 1371
Size: 280 267 54 381 389
Fragments arranged by size:
389 381 280 267 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1204 1215 1371
Size: 1204 11 156
Fragments arranged by size:
1204 156 11
EcoICRI GAG'CTC
Cuts at: 0 1119 1371
Size: 1119 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 187 358 943 1118 1326 1371
Size: 172 15 171 585 175 208 45
Fragments arranged by size:
585 208 175 172 171 45 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 1371
Size: 154 1217
EcoO109I rG'GnC_Cy
Cuts at: 0 63 708 894 895 1371
Size: 63 645 186 1 476
Fragments arranged by size:
645 476 186 63 1
EcoRV GAT'ATC
Cuts at: 0 981 1371
Size: 981 390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1128 1371
Size: 1128 243
HaeII r_GCGC'y
Cuts at: 0 53 1215 1283 1371
Size: 53 1162 68 88
Fragments arranged by size:
1162 88 68 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 1371
Size: 607 32 732
Fragments arranged by size:
732 607 32
KasI G'GCGC_C
Cuts at: 0 49 1279 1371
Size: 49 1230 92
Fragments arranged by size:
1230 92 49
KpnI G_GTAC'C
Cuts at: 0 796 1371
Size: 796 575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1371
Size: 593 778
MscI TGG'CCA
Cuts at: 0 219 1371
Size: 219 1152
MslI CAynn'nnrTG
Cuts at: 0 532 811 879 1371
Size: 532 279 68 492
Fragments arranged by size:
532 492 279 68
MspA1I CmG'CkG
Cuts at: 0 18 29 165 311 571 605 1371
Size: 18 11 136 146 260 34 766
Fragments arranged by size:
766 260 146 136 34 18 11
NaeI GCC'GGC
Cuts at: 0 74 1371
Size: 74 1297
NarI GG'CG_CC
Cuts at: 0 50 1280 1371
Size: 50 1230 91
Fragments arranged by size:
1230 91 50
NcoI C'CATG_G
Cuts at: 0 653 809 1371
Size: 653 156 562
Fragments arranged by size:
653 562 156
NgoMIV G'CCGG_C
Cuts at: 0 72 1371
Size: 72 1299
NheI G'CTAG_C
Cuts at: 0 1014 1371
Size: 1014 357
NspI r_CATG'y
Cuts at: 0 537 1371
Size: 537 834
PflMI CCAn_nnn'nTGG
Cuts at: 0 815 1371
Size: 815 556
PmlI CAC'GTG
Cuts at: 0 1286 1371
Size: 1286 85
PshAI GACnn'nnGTC
Cuts at: 0 998 1315 1371
Size: 998 317 56
Fragments arranged by size:
998 317 56
PspOMI G'GGCC_C
Cuts at: 0 62 894 1371
Size: 62 832 477
Fragments arranged by size:
832 477 62
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 1371
Size: 1234 32 105
Fragments arranged by size:
1234 105 32
PstI C_TGCA'G
Cuts at: 0 101 190 1226 1371
Size: 101 89 1036 145
Fragments arranged by size:
1036 145 101 89
PvuII CAG'CTG
Cuts at: 0 165 311 1371
Size: 165 146 1060
Fragments arranged by size:
1060 165 146
SacI G_AGCT'C
Cuts at: 0 1121 1371
Size: 1121 250
SapI GCTCTTCn'nnn_
Cuts at: 0 280 1371
Size: 280 1091
SbfI CC_TGCA'GG
Cuts at: 0 1226 1371
Size: 1226 145
ScaI AGT'ACT
Cuts at: 0 760 1371
Size: 760 611
SfcI C'TryA_G
Cuts at: 0 97 186 1222 1371
Size: 97 89 1036 149
Fragments arranged by size:
1036 149 97 89
SfoI GGC'GCC
Cuts at: 0 51 1281 1371
Size: 51 1230 90
Fragments arranged by size:
1230 90 51
SmlI C'TyrA_G
Cuts at: 0 1008 1371
Size: 1008 363
SspI AAT'ATT
Cuts at: 0 670 1371
Size: 670 701
StyI C'CwwG_G
Cuts at: 0 66 653 809 1371
Size: 66 587 156 562
Fragments arranged by size:
587 562 156 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 839 1371
Size: 839 532
TatI w'GTAC_w
Cuts at: 0 719 758 1053 1371
Size: 719 39 295 318
Fragments arranged by size:
719 318 295 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 835 867 1371
Size: 835 32 504
Fragments arranged by size:
835 504 32
XmnI GAAnn'nnTTC
Cuts at: 0 760 1371
Size: 760 611
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI Bme1580I BmtI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PflMI
PmlI PshAI PspOMI PsrI PstI PvuII SacI SapI
SbfI ScaI SfcI SfoI SmlI SspI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BplI BsaI BsaBI BsiEI BsiWI BsmI
BspHI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EagI EcoRI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI NdeI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PspXI PvuI RsrII SacII SalI SanDI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI