Restriction map for
pFN21AE1915
(Linear) (Six-base) MAPSORT of: KIEE1915.seq Check: 9393 from: 1 to: 1356
KIEE1915.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AatII G_ACGT'C
Cuts at: 0 719 1356
Size: 719 637
AccI GT'mk_AC
Cuts at: 0 469 1356
Size: 469 887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 505 1356
Size: 358 147 851
Fragments arranged by size:
851 358 147
AgeI A'CCGG_T
Cuts at: 0 1280 1356
Size: 1280 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 788 1356
Size: 788 568
AlwNI CAG_nnn'CTG
Cuts at: 0 478 1356
Size: 478 878
ApaLI G'TGCA_C
Cuts at: 0 622 1356
Size: 622 734
ApoI r'AATT_y
Cuts at: 0 544 649 768 1273 1356
Size: 544 105 119 505 83
Fragments arranged by size:
544 505 119 105 83
BamHI G'GATC_C
Cuts at: 0 1015 1356
Size: 1015 341
BanI G'GyrC_C
Cuts at: 0 6 841 1356
Size: 6 835 515
Fragments arranged by size:
835 515 6
BanII G_rGCy'C
Cuts at: 0 218 1356
Size: 218 1138
BbeI G_GCGC'C
Cuts at: 0 10 845 1356
Size: 10 835 511
Fragments arranged by size:
835 511 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 806 840 1150 1184 1356
Size: 806 34 310 34 172
Fragments arranged by size:
806 310 172 34 34
BfrBI ATG'CAT
Cuts at: 0 96 1356
Size: 96 1260
Bme1580I G_kGCm'C
Cuts at: 0 626 1356
Size: 626 730
BmtI G_CTAG'C
Cuts at: 0 142 1356
Size: 142 1214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 361 653 680 856 1356
Size: 202 159 292 27 176 500
Fragments arranged by size:
500 292 202 176 159 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1157 1356
Size: 432 725 199
Fragments arranged by size:
725 432 199
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 1356
Size: 316 1040
BsaAI yAC'GTr
Cuts at: 0 1206 1356
Size: 1206 150
BsaHI Gr'CG_yC
Cuts at: 0 7 716 842 1356
Size: 7 709 126 514
Fragments arranged by size:
709 514 126 7
BsaWI w'CCGG_w
Cuts at: 0 426 1280 1356
Size: 426 854 76
Fragments arranged by size:
854 426 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 653 683 1356
Size: 334 30 289 30 673
Fragments arranged by size:
673 334 289 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 811 1045 1356
Size: 250 561 234 311
Fragments arranged by size:
561 311 250 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 770 1356
Size: 725 45 586
Fragments arranged by size:
725 586 45
BsiHKAI G_wGCw'C
Cuts at: 0 218 626 1356
Size: 218 408 730
Fragments arranged by size:
730 408 218
Bsp1286I G_dGCh'C
Cuts at: 0 218 626 1356
Size: 218 408 730
Fragments arranged by size:
730 408 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 792 1356
Size: 792 564
BsrBI CCG'CTC
Cuts at: 0 1188 1356
Size: 1188 168
BsrDI GCAATG_nn'
Cuts at: 0 624 1356
Size: 624 732
BsrFI r'CCGG_y
Cuts at: 0 1042 1280 1356
Size: 1042 238 76
Fragments arranged by size:
1042 238 76
BssSI C'ACGA_G
Cuts at: 0 593 1356
Size: 593 763
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1316 1356
Size: 1316 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 997 1356
Size: 997 359
BstYI r'GATC_y
Cuts at: 0 1015 1356
Size: 1015 341
BtgI C'CryG_G
Cuts at: 0 960 1356
Size: 960 396
DraIII CAC_nnn'GTG
Cuts at: 0 1316 1356
Size: 1316 40
EaeI y'GGCC_r
Cuts at: 0 53 1208 1356
Size: 53 1155 148
Fragments arranged by size:
1155 148 53
EarI CTCTTCn'nnn_
Cuts at: 0 700 1356
Size: 700 656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 1356
Size: 451 905
EcoICRI GAG'CTC
Cuts at: 0 216 1356
Size: 216 1140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 358 361 505 653 680 856 1356
Size: 202 156 3 144 148 27 176 500
Fragments arranged by size:
500 202 176 156 148 144 27 3
EcoO109I rG'GnC_Cy
Cuts at: 0 283 1356
Size: 283 1073
EcoRI G'AATT_C
Cuts at: 0 1273 1356
Size: 1273 83
HaeII r_GCGC'y
Cuts at: 0 10 845 1356
Size: 10 835 511
Fragments arranged by size:
835 511 10
HincII GTy'rAC
Cuts at: 0 470 987 1356
Size: 470 517 369
Fragments arranged by size:
517 470 369
HindIII A'AGCT_T
Cuts at: 0 290 700 1356
Size: 290 410 656
Fragments arranged by size:
656 410 290
KasI G'GCGC_C
Cuts at: 0 6 841 1356
Size: 6 835 515
Fragments arranged by size:
835 515 6
MspA1I CmG'CkG
Cuts at: 0 58 150 567 1356
Size: 58 92 417 789
Fragments arranged by size:
789 417 92 58
NarI GG'CG_CC
Cuts at: 0 7 842 1356
Size: 7 835 514
Fragments arranged by size:
835 514 7
NheI G'CTAG_C
Cuts at: 0 138 1356
Size: 138 1218
NsiI A_TGCA'T
Cuts at: 0 98 1356
Size: 98 1258
NspI r_CATG'y
Cuts at: 0 96 382 1218 1356
Size: 96 286 836 138
Fragments arranged by size:
836 286 138 96
PasI CC'CwG_GG
Cuts at: 0 1021 1356
Size: 1021 335
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1356
Size: 252 1104
PmlI CAC'GTG
Cuts at: 0 1206 1356
Size: 1206 150
PpuMI rG'GwC_Cy
Cuts at: 0 283 1356
Size: 283 1073
PshAI GACnn'nnGTC
Cuts at: 0 413 1356
Size: 413 943
PstI C_TGCA'G
Cuts at: 0 767 1309 1356
Size: 767 542 47
Fragments arranged by size:
767 542 47
PvuII CAG'CTG
Cuts at: 0 150 567 1356
Size: 150 417 789
Fragments arranged by size:
789 417 150
SacI G_AGCT'C
Cuts at: 0 218 1356
Size: 218 1138
SalI G'TCGA_C
Cuts at: 0 468 1356
Size: 468 888
SanDI GG'GwC_CC
Cuts at: 0 283 1356
Size: 283 1073
ScaI AGT'ACT
Cuts at: 0 681 1356
Size: 681 675
SfcI C'TryA_G
Cuts at: 0 763 922 1305 1356
Size: 763 159 383 51
Fragments arranged by size:
763 383 159 51
SfoI GGC'GCC
Cuts at: 0 8 843 1356
Size: 8 835 513
Fragments arranged by size:
835 513 8
SgrAI Cr'CCGG_yG
Cuts at: 0 1280 1356
Size: 1280 76
SmlI C'TyrA_G
Cuts at: 0 411 1172 1356
Size: 411 761 184
Fragments arranged by size:
761 411 184
SphI G_CATG'C
Cuts at: 0 96 1356
Size: 96 1260
StuI AGG'CCT
Cuts at: 0 955 1356
Size: 955 401
StyI C'CwwG_G
Cuts at: 0 200 1356
Size: 200 1156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1013 1356
Size: 1013 343
TatI w'GTAC_w
Cuts at: 0 381 679 1356
Size: 381 298 677
Fragments arranged by size:
677 381 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 653 685 1000 1032 1356
Size: 653 32 315 32 324
Fragments arranged by size:
653 324 315 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 958 1356
Size: 852 106 398
Fragments arranged by size:
852 398 106
XmnI GAAnn'nnTTC
Cuts at: 0 176 1356
Size: 176 1180
ZraI GAC'GTC
Cuts at: 0 717 1356
Size: 717 639
Enzymes that do cut:
AatII AccI AcuI AgeI AlfI AlwNI ApaLI ApoI
BamHI BanI BanII BbeI BcgI BfrBI Bme1580I BmtI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BtgI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI HaeII HincII HindIII KasI
MspA1I NarI NheI NsiI NspI PasI PflMI PmlI
PpuMI PshAI PstI PvuII SacI SalI SanDI ScaI
SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI AleI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BbsI BbvCI BciVI BclI BglI BglII BlpI BmgBI
BmrI BplI Bpu10I BsaBI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EcoNI EcoRV FalI FseI FspI FspAI Hin4I HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NcoI
NdeI NgoMIV NotI NruI PacI PciI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SapI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI