(Linear) (Six-base) MAPSORT of: KIEE1909.seq  Check: 47  from: 1  to: 1332



KIEE1909.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:50  ..

AccI GT'mk_AC

Cuts at:      0     419    1115    1332 
   Size:        419     696     217

  Fragments arranged by size: 

                 696     419     217
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     521     884    1332 
   Size:        259     262     363     448

  Fragments arranged by size: 

                 448     363     262     259
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1230    1332 
   Size:       1230     102
 
ApoI r'AATT_y

Cuts at:      0     175     493    1162    1332 
   Size:        175     318     669     170

  Fragments arranged by size: 

                 669     318     175     170
 
BanII G_rGCy'C

Cuts at:      0     542    1332 
   Size:        542     790
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      14    1332 
   Size:         14    1318
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      73     595    1332 
   Size:         73     522     737

  Fragments arranged by size: 

                 737     522      73
 
Bpu10I CC'TnA_GC

Cuts at:      0    1307    1332 
   Size:       1307      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     467    1332 
   Size:        467     865
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     520     919    1332 
   Size:        520     399     413

  Fragments arranged by size: 

                 520     413     399
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     738    1332 
   Size:        738     594
 
BsiHKAI G_wGCw'C

Cuts at:      0     542    1332 
   Size:        542     790
 
Bsp1286I G_dGCh'C

Cuts at:      0     542    1332 
   Size:        542     790
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     817    1084    1332 
   Size:        817     267     248

  Fragments arranged by size: 

                 817     267     248
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     877    1332 
   Size:        877     455
 
BstYI r'GATC_y

Cuts at:      0     376     780    1226    1332 
   Size:        376     404     446     106

  Fragments arranged by size: 

                 446     404     376     106
 
BtsI GCAGTG_nn'

Cuts at:      0     750    1332 
   Size:        750     582
 
DraI TTT'AAA

Cuts at:      0     662    1332 
   Size:        662     670
 
EarI CTCTTCn'nnn_

Cuts at:      0      42     497    1332 
   Size:         42     455     835

  Fragments arranged by size: 

                 835     455      42
 
EcoICRI GAG'CTC

Cuts at:      0     540    1332 
   Size:        540     792
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     521     884    1332 
   Size:        259     262     363     448

  Fragments arranged by size: 

                 448     363     262     259
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      39    1306    1332 
   Size:         39    1267      26

  Fragments arranged by size: 

                1267      39      26
 
EcoRI G'AATT_C

Cuts at:      0     493    1332 
   Size:        493     839
 
EcoRV GAT'ATC

Cuts at:      0     616    1332 
   Size:        616     716
 
HaeII r_GCGC'y

Cuts at:      0     899    1332 
   Size:        899     433
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741    1332 
   Size:        709      32     591

  Fragments arranged by size: 

                 709     591      32
 
HincII GTy'rAC

Cuts at:      0     192    1332 
   Size:        192    1140
 
MfeI C'AATT_G

Cuts at:      0     214     637    1332 
   Size:        214     423     695

  Fragments arranged by size: 

                 695     423     214
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     677    1332 
   Size:         63     614     655

  Fragments arranged by size: 

                 655     614      63
 
MslI CAynn'nnrTG

Cuts at:      0     251    1332 
   Size:        251    1081
 
NspI r_CATG'y

Cuts at:      0     127    1030    1045    1332 
   Size:        127     903      15     287

  Fragments arranged by size: 

                 903     287     127      15
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     759     791    1332 
   Size:        759      32     541

  Fragments arranged by size: 

                 759     541      32
 
PsiI TTA'TAA

Cuts at:      0      94    1332 
   Size:         94    1238
 
PstI C_TGCA'G

Cuts at:      0    1274    1332 
   Size:       1274      58
 
SacI G_AGCT'C

Cuts at:      0     542    1332 
   Size:        542     790
 
SapI GCTCTTCn'nnn_

Cuts at:      0      42    1332 
   Size:         42    1290
 
SfcI C'TryA_G

Cuts at:      0    1270    1332 
   Size:       1270      62
 
SspI AAT'ATT

Cuts at:      0     607    1332 
   Size:        607     725
 
StyI C'CwwG_G

Cuts at:      0     316    1332 
   Size:        316    1016
 
Tth111I GACn'n_nGTC

Cuts at:      0     415    1332 
   Size:        415     917
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     690    1332 
   Size:        690     642
 
XmnI GAAnn'nnTTC

Cuts at:      0     311    1332 
   Size:        311    1021
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BanII     BbsI     BplI   Bpu10I
     BsaI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI    BstXI    BstYI
     BtsI     DraI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
    HaeII    Hin4I   HincII     MfeI     MmeI     MslI     NspI     PpiI
     PsiI     PstI     SacI     SapI     SfcI     SspI     StyI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI EcoO109I     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TatI     TstI     XbaI     XhoI
     XmaI     ZraI