Restriction map for
pFN21AE1906
(Linear) (Six-base) MAPSORT of: KIEE1906.seq Check: 8212 from: 1 to: 1326
KIEE1906.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AatII G_ACGT'C
Cuts at: 0 485 1326
Size: 485 841
AccI GT'mk_AC
Cuts at: 0 64 1326
Size: 64 1262
Acc65I G'GTAC_C
Cuts at: 0 422 1326
Size: 422 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 447 1326
Size: 163 284 879
Fragments arranged by size:
879 284 163
AfeI AGC'GCT
Cuts at: 0 21 631 1326
Size: 21 610 695
Fragments arranged by size:
695 610 21
AleI CACnn'nnGTG
Cuts at: 0 8 1326
Size: 8 1318
AlwNI CAG_nnn'CTG
Cuts at: 0 132 1326
Size: 132 1194
AvaI C'yCGr_G
Cuts at: 0 178 1326
Size: 178 1148
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 761 794 1326
Size: 761 33 532
Fragments arranged by size:
761 532 33
BanI G'GyrC_C
Cuts at: 0 422 847 1326
Size: 422 425 479
Fragments arranged by size:
479 425 422
BanII G_rGCy'C
Cuts at: 0 179 1326
Size: 179 1147
BbsI GAAGACnn'nnnn_
Cuts at: 0 170 919 1326
Size: 170 749 407
Fragments arranged by size:
749 407 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 60 90 94 124 1326
Size: 60 30 4 30 1202
Fragments arranged by size:
1202 60 30 30 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 1079 1326
Size: 1079 247
BclI T'GATC_A
Cuts at: 0 870 1326
Size: 870 456
BlpI GC'TnA_GC
Cuts at: 0 16 55 1326
Size: 16 39 1271
Fragments arranged by size:
1271 39 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 1326
Size: 349 977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 381 1145 1326
Size: 217 164 764 181
Fragments arranged by size:
764 217 181 164
Bpu10I CC'TnA_GC
Cuts at: 0 133 173 1174 1326
Size: 133 40 1001 152
Fragments arranged by size:
1001 152 133 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 976 1326
Size: 583 393 350
Fragments arranged by size:
583 393 350
BsaBI GATnn'nnATC
Cuts at: 0 205 1326
Size: 205 1121
BsaHI Gr'CG_yC
Cuts at: 0 482 1326
Size: 482 844
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 1326
Size: 291 1035
BseYI C'CCAG_C
Cuts at: 0 626 1326
Size: 626 700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 753 1042 1326
Size: 753 289 284
Fragments arranged by size:
753 289 284
BsiEI CG_ry'CG
Cuts at: 0 50 260 1326
Size: 50 210 1066
Fragments arranged by size:
1066 210 50
BsiHKAI G_wGCw'C
Cuts at: 0 397 416 1326
Size: 397 19 910
Fragments arranged by size:
910 397 19
BsmI GAATG_Cn'
Cuts at: 0 507 1326
Size: 507 819
BsmBI CGTCTCn'nnnn_
Cuts at: 0 489 1326
Size: 489 837
Bsp1286I G_dGCh'C
Cuts at: 0 179 397 416 1326
Size: 179 218 19 910
Fragments arranged by size:
910 218 179 19
BspHI T'CATG_A
Cuts at: 0 705 1326
Size: 705 621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 76 826 1326
Size: 76 750 500
Fragments arranged by size:
750 500 76
BsrDI GCAATG_nn'
Cuts at: 0 497 1326
Size: 497 829
BsrFI r'CCGG_y
Cuts at: 0 974 1326
Size: 974 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 392 832 1326
Size: 392 440 494
Fragments arranged by size:
494 440 392
BstYI r'GATC_y
Cuts at: 0 128 1068 1326
Size: 128 940 258
Fragments arranged by size:
940 258 128
BtgI C'CryG_G
Cuts at: 0 120 1326
Size: 120 1206
DraI TTT'AAA
Cuts at: 0 744 1326
Size: 744 582
EaeI y'GGCC_r
Cuts at: 0 257 1326
Size: 257 1069
EagI C'GGCC_G
Cuts at: 0 257 1326
Size: 257 1069
EarI CTCTTCn'nnn_
Cuts at: 0 281 1326
Size: 281 1045
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 993 1326
Size: 993 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 217 381 447 1145 1326
Size: 163 54 164 66 698 181
Fragments arranged by size:
698 181 164 163 66 54
EcoO109I rG'GnC_Cy
Cuts at: 0 1170 1326
Size: 1170 156
FspI TGC'GCA
Cuts at: 0 100 1326
Size: 100 1226
HaeII r_GCGC'y
Cuts at: 0 23 633 1326
Size: 23 610 693
Fragments arranged by size:
693 610 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 467 499 601 633 835 867
Size: 21 32 414 32 102 32 202 32
Cuts at: 867 906 938 1326
Size: 39 32 388
Fragments arranged by size:
414 388 202 102 39 32 32 32
32 32 21
HindIII A'AGCT_T
Cuts at: 0 750 1326
Size: 750 576
KpnI G_GTAC'C
Cuts at: 0 426 1326
Size: 426 900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1326
Size: 646 680
MslI CAynn'nnrTG
Cuts at: 0 8 1326
Size: 8 1318
MspA1I CmG'CkG
Cuts at: 0 122 1326
Size: 122 1204
NotI GC'GGCC_GC
Cuts at: 0 257 1326
Size: 257 1069
NspI r_CATG'y
Cuts at: 0 324 1133 1326
Size: 324 809 193
Fragments arranged by size:
809 324 193
PfoI T'CCnGG_A
Cuts at: 0 233 1326
Size: 233 1093
PpuMI rG'GwC_Cy
Cuts at: 0 1170 1326
Size: 1170 156
PshAI GACnn'nnGTC
Cuts at: 0 957 1326
Size: 957 369
PstI C_TGCA'G
Cuts at: 0 73 1326
Size: 73 1253
SacII CC_GC'GG
Cuts at: 0 123 1326
Size: 123 1203
ScaI AGT'ACT
Cuts at: 0 292 1326
Size: 292 1034
SfcI C'TryA_G
Cuts at: 0 69 450 948 1326
Size: 69 381 498 378
Fragments arranged by size:
498 381 378 69
SmlI C'TyrA_G
Cuts at: 0 598 955 1326
Size: 598 357 371
Fragments arranged by size:
598 371 357
SphI G_CATG'C
Cuts at: 0 1133 1326
Size: 1133 193
StyI C'CwwG_G
Cuts at: 0 299 1326
Size: 299 1027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1290 1326
Size: 1290 36
TatI w'GTAC_w
Cuts at: 0 290 1326
Size: 290 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 972 1004 1060 1092 1326
Size: 972 32 56 32 234
Fragments arranged by size:
972 234 56 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 415 1326
Size: 415 911
XmnI GAAnn'nnTTC
Cuts at: 0 713 1326
Size: 713 613
ZraI GAC'GTC
Cuts at: 0 483 1326
Size: 483 843
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AleI AlwNI AvaI
BaeI BanI BanII BbsI BcgI BciVI BclI BlpI
BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstYI BtgI DraI EaeI EagI
EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I HindIII
KpnI MmeI MslI MspA1I NotI NspI PfoI PpuMI
PshAI PstI SacII ScaI SfcI SmlI SphI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BsaI BsaAI BsaWI BsaXI BsiWI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI