Restriction map for
pFN21AE1902
(Linear) (Six-base) MAPSORT of: KIEE1902.seq Check: 2790 from: 1 to: 1287
KIEE1902.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AatII G_ACGT'C
Cuts at: 0 425 1287
Size: 425 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1218 1287
Size: 1192 26 69
Fragments arranged by size:
1192 69 26
AfeI AGC'GCT
Cuts at: 0 685 1287
Size: 685 602
AflIII A'CryG_T
Cuts at: 0 488 1287
Size: 488 799
AhdI GACnn_n'nnGTC
Cuts at: 0 15 930 1287
Size: 15 915 357
Fragments arranged by size:
915 357 15
AlwNI CAG_nnn'CTG
Cuts at: 0 90 330 333 501 843 894 1287
Size: 90 240 3 168 342 51 393
Fragments arranged by size:
393 342 240 168 90 51 3
AvaI C'yCGr_G
Cuts at: 0 5 12 407 760 863 1031 1287
Size: 5 7 395 353 103 168 256
Fragments arranged by size:
395 353 256 168 103 7 5
BanI G'GyrC_C
Cuts at: 0 250 1110 1287
Size: 250 860 177
Fragments arranged by size:
860 250 177
BanII G_rGCy'C
Cuts at: 0 722 761 960 1259 1287
Size: 722 39 199 299 28
Fragments arranged by size:
722 299 199 39 28
BbeI G_GCGC'C
Cuts at: 0 254 1287
Size: 254 1033
BbsI GAAGACnn'nnnn_
Cuts at: 0 668 1287
Size: 668 619
BglI GCCn_nnn'nGGC
Cuts at: 0 154 259 1287
Size: 154 105 1028
Fragments arranged by size:
1028 154 105
BglII A'GATC_T
Cuts at: 0 779 1287
Size: 779 508
BlpI GC'TnA_GC
Cuts at: 0 334 1287
Size: 334 953
Bme1580I G_kGCm'C
Cuts at: 0 587 1216 1287
Size: 587 629 71
Fragments arranged by size:
629 587 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 1145 1287
Size: 1145 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 223 837 1000 1038 1143 1287
Size: 184 39 614 163 38 105 144
Fragments arranged by size:
614 184 163 144 105 39 38
Bpu10I CC'TnA_GC
Cuts at: 0 733 1287
Size: 733 554
BsaI GGTCTCn'nnnn_
Cuts at: 0 442 1287
Size: 442 845
BsaAI yAC'GTr
Cuts at: 0 711 1287
Size: 711 576
BsaHI Gr'CG_yC
Cuts at: 0 125 173 251 422 1043 1287
Size: 125 48 78 171 621 244
Fragments arranged by size:
621 244 171 125 78 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 396 1249 1287
Size: 46 350 853 38
Fragments arranged by size:
853 350 46 38
BseYI C'CCAG_C
Cuts at: 0 26 1060 1142 1287
Size: 26 1034 82 145
Fragments arranged by size:
1034 145 82 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 907 988 1287
Size: 45 862 81 299
Fragments arranged by size:
862 299 81 45
BsiEI CG_ry'CG
Cuts at: 0 948 1050 1287
Size: 948 102 237
Fragments arranged by size:
948 237 102
BsiHKAI G_wGCw'C
Cuts at: 0 1259 1287
Size: 1259 28
Bsp1286I G_dGCh'C
Cuts at: 0 587 722 761 960 1216 1259 1287
Size: 587 135 39 199 256 43 28
Fragments arranged by size:
587 256 199 135 43 39 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 198 1287
Size: 198 1089
BsrBI CCG'CTC
Cuts at: 0 434 882 1287
Size: 434 448 405
Fragments arranged by size:
448 434 405
BsrFI r'CCGG_y
Cuts at: 0 1045 1287
Size: 1045 242
BssHII G'CGCG_C
Cuts at: 0 42 214 402 1287
Size: 42 172 188 885
Fragments arranged by size:
885 188 172 42
BssSI C'ACGA_G
Cuts at: 0 73 349 754 1287
Size: 73 276 405 533
Fragments arranged by size:
533 405 276 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 1287
Size: 330 957
BstXI CCAn_nnnn'nTGG
Cuts at: 0 513 1083 1287
Size: 513 570 204
Fragments arranged by size:
570 513 204
BstYI r'GATC_y
Cuts at: 0 746 779 1287
Size: 746 33 508
Fragments arranged by size:
746 508 33
Bsu36I CC'TnA_GG
Cuts at: 0 1261 1287
Size: 1261 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 1287
Size: 117 1170
BtsI GCAGTG_nn'
Cuts at: 0 1218 1234 1287
Size: 1218 16 53
Fragments arranged by size:
1218 53 16
DraIII CAC_nnn'GTG
Cuts at: 0 1011 1287
Size: 1011 276
EaeI y'GGCC_r
Cuts at: 0 155 440 503 1047 1085 1287
Size: 155 285 63 544 38 202
Fragments arranged by size:
544 285 202 155 63 38
EagI C'GGCC_G
Cuts at: 0 1047 1287
Size: 1047 240
EarI CTCTTCn'nnn_
Cuts at: 0 800 1247 1287
Size: 800 447 40
Fragments arranged by size:
800 447 40
EcoICRI GAG'CTC
Cuts at: 0 1257 1287
Size: 1257 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 223 837 1000 1038 1143 1192 1218
Size: 184 39 614 163 38 105 49 26
Cuts at: 1218 1287
Size: 69
Fragments arranged by size:
614 184 163 105 69 49 39 38
26
EcoO109I rG'GnC_Cy
Cuts at: 0 9 36 1139 1203 1287
Size: 9 27 1103 64 84
Fragments arranged by size:
1103 84 64 27 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 942 1287
Size: 942 345
FspI TGC'GCA
Cuts at: 0 728 1287
Size: 728 559
HaeII r_GCGC'y
Cuts at: 0 254 687 1287
Size: 254 433 600
Fragments arranged by size:
600 433 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 916 948 1287
Size: 1 32 883 32 339
Fragments arranged by size:
883 339 32 32 1
KasI G'GCGC_C
Cuts at: 0 250 1287
Size: 250 1037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 782 857 1287
Size: 548 234 75 430
Fragments arranged by size:
548 430 234 75
MscI TGG'CCA
Cuts at: 0 442 505 1287
Size: 442 63 782
Fragments arranged by size:
782 442 63
MslI CAynn'nnrTG
Cuts at: 0 578 1287
Size: 578 709
MspA1I CmG'CkG
Cuts at: 0 49 87 231 330 459 1287
Size: 49 38 144 99 129 828
Fragments arranged by size:
828 144 129 99 49 38
NaeI GCC'GGC
Cuts at: 0 1047 1287
Size: 1047 240
NarI GG'CG_CC
Cuts at: 0 251 1287
Size: 251 1036
NgoMIV G'CCGG_C
Cuts at: 0 1045 1287
Size: 1045 242
NspI r_CATG'y
Cuts at: 0 492 532 1270 1287
Size: 492 40 738 17
Fragments arranged by size:
738 492 40 17
PciI A'CATG_T
Cuts at: 0 488 1287
Size: 488 799
PfoI T'CCnGG_A
Cuts at: 0 221 853 1015 1287
Size: 221 632 162 272
Fragments arranged by size:
632 272 221 162
PpuMI rG'GwC_Cy
Cuts at: 0 9 1139 1203 1287
Size: 9 1130 64 84
Fragments arranged by size:
1130 84 64 9
PstI C_TGCA'G
Cuts at: 0 104 746 1164 1229 1287
Size: 104 642 418 65 58
Fragments arranged by size:
642 418 104 65 58
PvuI CG_AT'CG
Cuts at: 0 948 1287
Size: 948 339
PvuII CAG'CTG
Cuts at: 0 49 231 330 459 1287
Size: 49 182 99 129 828
Fragments arranged by size:
828 182 129 99 49
SacI G_AGCT'C
Cuts at: 0 1259 1287
Size: 1259 28
SanDI GG'GwC_CC
Cuts at: 0 9 1139 1287
Size: 9 1130 148
Fragments arranged by size:
1130 148 9
SapI GCTCTTCn'nnn_
Cuts at: 0 1247 1287
Size: 1247 40
SbfI CC_TGCA'GG
Cuts at: 0 104 1287
Size: 104 1183
SexAI A'CCwGG_T
Cuts at: 0 1007 1287
Size: 1007 280
SfcI C'TryA_G
Cuts at: 0 100 742 1160 1225 1287
Size: 100 642 418 65 62
Fragments arranged by size:
642 418 100 65 62
SfoI GGC'GCC
Cuts at: 0 252 1287
Size: 252 1035
SmaI CCC'GGG
Cuts at: 0 7 14 409 865 1287
Size: 7 7 395 456 422
Fragments arranged by size:
456 422 395 7 7
SmlI C'TyrA_G
Cuts at: 0 1031 1287
Size: 1031 256
SphI G_CATG'C
Cuts at: 0 532 1287
Size: 532 755
SrfI GCCC'GGGC
Cuts at: 0 409 1287
Size: 409 878
StuI AGG'CCT
Cuts at: 0 1128 1287
Size: 1128 159
StyI C'CwwG_G
Cuts at: 0 437 1287
Size: 437 850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 997 1029 1195 1227 1287
Size: 997 32 166 32 60
Fragments arranged by size:
997 166 60 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1137 1287
Size: 1137 150
XhoI C'TCGA_G
Cuts at: 0 1031 1287
Size: 1031 256
XmaI C'CCGG_G
Cuts at: 0 5 12 407 863 1287
Size: 5 7 395 456 424
Fragments arranged by size:
456 424 395 7 5
ZraI GAC'GTC
Cuts at: 0 423 1287
Size: 423 864
Enzymes that do cut:
AatII AcuI AfeI AflIII AhdI AlwNI AvaI BanI
BanII BbeI BbsI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgZI BtsI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PpuMI PstI PvuI PvuII
SacI SanDI SapI SbfI SexAI SfcI SfoI SmaI
SmlI SphI SrfI StuI StyI TstI Tth111I XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI
BmtI BplI BpuEI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstZ17I
BtgI ClaI CspCI DraI DrdI EciI EcoNI EcoRI
EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI RsrII SacII SalI ScaI SfiI SgfI
SgrAI SnaBI SpeI SspI SwaI TaqII TatI XbaI
XcmI XmnI