(Linear) (Six-base) MAPSORT of: KIEE1901.seq  Check: 9663  from: 1  to: 1287



KIEE1901.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:50  ..

Acc65I G'GTAC_C

Cuts at:      0    1210    1287 
   Size:       1210      77
 
ApoI r'AATT_y

Cuts at:      0     862    1287 
   Size:        862     425
 
AseI AT'TA_AT

Cuts at:      0     157    1287 
   Size:        157    1130
 
BamHI G'GATC_C

Cuts at:      0      55    1287 
   Size:         55    1232
 
BanI G'GyrC_C

Cuts at:      0     372    1210    1287 
   Size:        372     838      77

  Fragments arranged by size: 

                 838     372      77
 
BanII G_rGCy'C

Cuts at:      0      39    1287 
   Size:         39    1248
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     287    1287 
   Size:        287    1000
 
BfrBI ATG'CAT

Cuts at:      0     327    1287 
   Size:        327     960
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294     506    1287 
   Size:        294     212     781

  Fragments arranged by size: 

                 781     294     212
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     683    1287 
   Size:        614      69     604

  Fragments arranged by size: 

                 614     604      69
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1033    1287 
   Size:       1033     254
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      14      26      44      56    1287 
   Size:         14      12      18      12    1231

  Fragments arranged by size: 

                1231      18      14      12      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1106    1287 
   Size:       1106     181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     821     914    1287 
   Size:        636     185      93     373

  Fragments arranged by size: 

                 636     373     185      93
 
Bsp1286I G_dGCh'C

Cuts at:      0      39    1287 
   Size:         39    1248
 
BsrBI CCG'CTC

Cuts at:      0       5     900    1287 
   Size:          5     895     387

  Fragments arranged by size: 

                 895     387       5
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     707    1287 
   Size:        707     580
 
BstYI r'GATC_y

Cuts at:      0      55     694    1287 
   Size:         55     639     593

  Fragments arranged by size: 

                 639     593      55
 
Bsu36I CC'TnA_GG

Cuts at:      0     731    1287 
   Size:        731     556
 
BtgI C'CryG_G

Cuts at:      0     376    1287 
   Size:        376     911
 
BtsI GCAGTG_nn'

Cuts at:      0     530     619    1044    1270    1287 
   Size:        530      89     425     226      17

  Fragments arranged by size: 

                 530     425     226      89      17
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     978    1013    1207    1242    1287 
   Size:        978      35     194      35      45

  Fragments arranged by size: 

                 978     194      45      35      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     631    1287 
   Size:        631     656
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     683    1287 
   Size:        614      69     604

  Fragments arranged by size: 

                 614     604      69
 
EcoRI G'AATT_C

Cuts at:      0     862    1287 
   Size:        862     425
 
EcoRV GAT'ATC

Cuts at:      0    1017    1287 
   Size:       1017     270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      40      72    1013    1045    1287 
   Size:         40      32     941      32     242

  Fragments arranged by size: 

                 941     242      40      32      32
 
HindIII A'AGCT_T

Cuts at:      0     788    1287 
   Size:        788     499
 
KpnI G_GTAC'C

Cuts at:      0    1214    1287 
   Size:       1214      73
 
MfeI C'AATT_G

Cuts at:      0     879    1287 
   Size:        879     408
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      12    1287 
   Size:         12    1275
 
MslI CAynn'nnrTG

Cuts at:      0     157    1287 
   Size:        157    1130
 
NsiI A_TGCA'T

Cuts at:      0     329    1287 
   Size:        329     958
 
NspI r_CATG'y

Cuts at:      0     331    1287 
   Size:        331     956
 
PfoI T'CCnGG_A

Cuts at:      0     776    1287 
   Size:        776     511
 
PstI C_TGCA'G

Cuts at:      0     465    1287 
   Size:        465     822
 
SexAI A'CCwGG_T

Cuts at:      0    1023    1287 
   Size:       1023     264
 
SfcI C'TryA_G

Cuts at:      0     113     461    1287 
   Size:        113     348     826

  Fragments arranged by size: 

                 826     348     113
 
SspI AAT'ATT

Cuts at:      0     642    1287 
   Size:        642     645
 
StyI C'CwwG_G

Cuts at:      0      98    1287 
   Size:         98    1189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     522     554    1251    1283    1287 
   Size:        522      32     697      32       4

  Fragments arranged by size: 

                 697     522      32      32       4
 
Tth111I GACn'n_nGTC

Cuts at:      0    1025    1287 
   Size:       1025     262
 
XmnI GAAnn'nnTTC

Cuts at:      0     650     683    1287 
   Size:        650      33     604

  Fragments arranged by size: 

                 650     604      33
 

 Enzymes that do cut:

   Acc65I     ApoI     AseI    BamHI     BanI    BanII     BbsI    BfrBI
     BmrI     BpmI     BsaI    BsaXI    BseRI     BsgI Bsp1286I    BsrBI
    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     EarI  Eco57MI
    EcoRI    EcoRV    Hin4I  HindIII     KpnI     MfeI     MmeI     MslI
     NsiI     NspI     PfoI     PstI    SexAI     SfcI     SspI     StyI
     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     MluI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI     XmaI
     ZraI