Restriction map for
pFN21AE1899
(Linear) (Six-base) MAPSORT of: KIEE1899.seq Check: 923 from: 1 to: 2475
KIEE1899.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 45 106 269 2475
Size: 45 61 163 2206
Fragments arranged by size:
2206 163 61 45
AccI GT'mk_AC
Cuts at: 0 2135 2475
Size: 2135 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1482 2475
Size: 592 890 993
Fragments arranged by size:
993 890 592
AflIII A'CryG_T
Cuts at: 0 215 217 2475
Size: 215 2 2258
Fragments arranged by size:
2258 215 2
AhdI GACnn_n'nnGTC
Cuts at: 0 1430 2475
Size: 1430 1045
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 533 565 2428 2460 2475
Size: 533 32 1863 32 15
Fragments arranged by size:
1863 533 32 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 324 327 1945 2475
Size: 324 3 1618 530
Fragments arranged by size:
1618 530 324 3
ApaI G_GGCC'C
Cuts at: 0 375 1358 2283 2475
Size: 375 983 925 192
Fragments arranged by size:
983 925 375 192
AvaI C'yCGr_G
Cuts at: 0 872 1582 2346 2475
Size: 872 710 764 129
Fragments arranged by size:
872 764 710 129
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 204 237 2475
Size: 204 33 2238
Fragments arranged by size:
2238 204 33
BanI G'GyrC_C
Cuts at: 0 1022 1365 1756 2340 2475
Size: 1022 343 391 584 135
Fragments arranged by size:
1022 584 391 343 135
BanII G_rGCy'C
Cuts at: 0 23 95 173 340 375 615 649 683
Size: 23 72 78 167 35 240 34 34
Cuts at: 683 1006 1358 2002 2283 2413 2475
Size: 323 352 644 281 130 62
Fragments arranged by size:
644 352 323 281 240 167 130 78
72 62 35 34 34 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 801 835 2475
Size: 801 34 1640
Fragments arranged by size:
1640 801 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 232 449 2475
Size: 232 217 2026
Fragments arranged by size:
2026 232 217
BglI GCCn_nnn'nGGC
Cuts at: 0 2338 2475
Size: 2338 137
Bme1580I G_kGCm'C
Cuts at: 0 375 597 898 1027 1358 1628 2283 2475
Size: 375 222 301 129 331 270 655 192
Fragments arranged by size:
655 375 331 301 270 222 192 129
BmgBI CAC'GTC
Cuts at: 0 276 2475
Size: 276 2199
BmrI ACTGGGnnnn_n'
Cuts at: 0 1021 1933 2475
Size: 1021 912 542
Fragments arranged by size:
1021 912 542
BmtI G_CTAG'C
Cuts at: 0 1874 2475
Size: 1874 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 654 961 1420 1588 1660 2059 2279
Size: 447 207 307 459 168 72 399 220
Cuts at: 2279 2332 2475
Size: 53 143
Fragments arranged by size:
459 447 399 307 220 207 168 143
72 53
Bpu10I CC'TnA_GC
Cuts at: 0 845 1510 1634 2475
Size: 845 665 124 841
Fragments arranged by size:
845 841 665 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1803 2397 2475
Size: 1803 594 78
Fragments arranged by size:
1803 594 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 2475
Size: 2475
BsaAI yAC'GTr
Cuts at: 0 218 2475
Size: 218 2257
BsaBI GATnn'nnATC
Cuts at: 0 965 2475
Size: 965 1510
BsaHI Gr'CG_yC
Cuts at: 0 707 1459 2475
Size: 707 752 1016
Fragments arranged by size:
1016 752 707
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 533 563 853 883 1137 1167 2428 2458
Size: 533 30 290 30 254 30 1261 30
Cuts at: 2458 2475
Size: 17
Fragments arranged by size:
1261 533 290 254 30 30 30 30
17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1129 1132 1135 1138 1264 2475
Size: 1129 3 3 3 126 1211
Fragments arranged by size:
1211 1129 126 3 3 3
BseYI C'CCAG_C
Cuts at: 0 313 349 649 702 804 1536 1601 2475
Size: 313 36 300 53 102 732 65 874
Fragments arranged by size:
874 732 313 300 102 65 53 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1370 2475
Size: 1370 1105
BsiHKAI G_wGCw'C
Cuts at: 0 173 947 1323 2002 2030 2413 2475
Size: 173 774 376 679 28 383 62
Fragments arranged by size:
774 679 383 376 173 62 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 108 1450 1707 2475
Size: 108 1342 257 768
Fragments arranged by size:
1342 768 257 108
Bsp1286I G_dGCh'C
Cuts at: 0 23 95 173 340 375 597 615 649
Size: 23 72 78 167 35 222 18 34
Cuts at: 649 683 898 947 1006 1027 1323 1358 1628
Size: 34 215 49 59 21 296 35 270
Cuts at: 1628 2002 2030 2283 2413 2475
Size: 374 28 253 130 62
Fragments arranged by size:
374 296 270 253 222 215 167 130
78 72 62 59 49 35 35 34
34 28 23 21 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 106 269 1247 2158 2475
Size: 45 61 163 978 911 317
Fragments arranged by size:
978 911 317 163 61 45
BsrDI GCAATG_nn'
Cuts at: 0 2406 2475
Size: 2406 69
BsrGI T'GTAC_A
Cuts at: 0 1734 2475
Size: 1734 741
BssSI C'ACGA_G
Cuts at: 0 820 2475
Size: 820 1655
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 699 2475
Size: 324 375 1776
Fragments arranged by size:
1776 375 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1006 1536 1566 1646 1773 2194 2475
Size: 1006 530 30 80 127 421 281
Fragments arranged by size:
1006 530 421 281 127 80 30
BstYI r'GATC_y
Cuts at: 0 966 1655 2475
Size: 966 689 820
Fragments arranged by size:
966 820 689
Bsu36I CC'TnA_GG
Cuts at: 0 2275 2475
Size: 2275 200
BtgI C'CryG_G
Cuts at: 0 2447 2475
Size: 2447 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 1092 1142 1455 2475
Size: 540 552 50 313 1020
Fragments arranged by size:
1020 552 540 313 50
BtsI GCAGTG_nn'
Cuts at: 0 1549 1842 1881 2130 2475
Size: 1549 293 39 249 345
Fragments arranged by size:
1549 345 293 249 39
DraIII CAC_nnn'GTG
Cuts at: 0 659 2475
Size: 659 1816
EaeI y'GGCC_r
Cuts at: 0 734 1054 1556 2163 2184 2199 2216 2475
Size: 734 320 502 607 21 15 17 259
Fragments arranged by size:
734 607 502 320 259 21 17 15
EarI CTCTTCn'nnn_
Cuts at: 0 1877 2356 2475
Size: 1877 479 119
Fragments arranged by size:
1877 479 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 1651 2475
Size: 163 1488 824
Fragments arranged by size:
1488 824 163
EcoICRI GAG'CTC
Cuts at: 0 171 2000 2411 2475
Size: 171 1829 411 64
Fragments arranged by size:
1829 411 171 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 592 654 961 1420 1482 1588 1660
Size: 447 145 62 307 459 62 106 72
Cuts at: 1660 2059 2279 2332 2475
Size: 399 220 53 143
Fragments arranged by size:
459 447 399 307 220 145 143 106
72 62 62 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 2475
Size: 52 2423
EcoO109I rG'GnC_Cy
Cuts at: 0 371 372 605 1098 1354 1786 1929 2252
Size: 371 1 233 493 256 432 143 323
Cuts at: 2252 2279 2280 2452 2475
Size: 27 1 172 23
Fragments arranged by size:
493 432 371 323 256 233 172 143
27 23 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 2475
Size: 357 2118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 853 885 1416 1448 1989 2021
Size: 132 32 689 32 531 32 541 32
Cuts at: 2021 2239 2271 2475
Size: 218 32 204
Fragments arranged by size:
689 541 531 218 204 132 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 483 2475
Size: 483 1992
MfeI C'AATT_G
Cuts at: 0 156 2475
Size: 156 2319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 431 2475
Size: 131 300 2044
Fragments arranged by size:
2044 300 131
MscI TGG'CCA
Cuts at: 0 1056 1558 2186 2201 2218 2475
Size: 1056 502 628 15 17 257
Fragments arranged by size:
1056 628 502 257 17 15
MslI CAynn'nnrTG
Cuts at: 0 398 1069 2461 2475
Size: 398 671 1392 14
Fragments arranged by size:
1392 671 398 14
MspA1I CmG'CkG
Cuts at: 0 189 324 1690 2475
Size: 189 135 1366 785
Fragments arranged by size:
1366 785 189 135
NheI G'CTAG_C
Cuts at: 0 1870 2475
Size: 1870 605
NspI r_CATG'y
Cuts at: 0 361 1329 1741 2067 2475
Size: 361 968 412 326 408
Fragments arranged by size:
968 412 408 361 326
PasI CC'CwG_GG
Cuts at: 0 368 1331 1373 1924 2475
Size: 368 963 42 551 551
Fragments arranged by size:
963 551 551 368 42
PfoI T'CCnGG_A
Cuts at: 0 836 1184 1232 2475
Size: 836 348 48 1243
Fragments arranged by size:
1243 836 348 48
PmlI CAC'GTG
Cuts at: 0 218 2475
Size: 218 2257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 533 565 1213 1245 2428 2460 2475
Size: 533 32 648 32 1183 32 15
Fragments arranged by size:
1183 648 533 32 32 32 15
PpuMI rG'GwC_Cy
Cuts at: 0 1786 1929 2252 2452 2475
Size: 1786 143 323 200 23
Fragments arranged by size:
1786 323 200 143 23
PshAI GACnn'nnGTC
Cuts at: 0 2209 2475
Size: 2209 266
PspOMI G'GGCC_C
Cuts at: 0 371 1354 2279 2475
Size: 371 983 925 196
Fragments arranged by size:
983 925 371 196
PstI C_TGCA'G
Cuts at: 0 56 1695 2475
Size: 56 1639 780
Fragments arranged by size:
1639 780 56
PvuII CAG'CTG
Cuts at: 0 189 324 1690 2475
Size: 189 135 1366 785
Fragments arranged by size:
1366 785 189 135
SacI G_AGCT'C
Cuts at: 0 173 2002 2413 2475
Size: 173 1829 411 62
Fragments arranged by size:
1829 411 173 62
SanDI GG'GwC_CC
Cuts at: 0 1786 1929 2452 2475
Size: 1786 143 523 23
Fragments arranged by size:
1786 523 143 23
SfcI C'TryA_G
Cuts at: 0 52 588 672 1691 2475
Size: 52 536 84 1019 784
Fragments arranged by size:
1019 784 536 84 52
SmlI C'TyrA_G
Cuts at: 0 1818 2412 2475
Size: 1818 594 63
Fragments arranged by size:
1818 594 63
StuI AGG'CCT
Cuts at: 0 1813 2475
Size: 1813 662
StyI C'CwwG_G
Cuts at: 0 1358 2475
Size: 1358 1117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1774 2475
Size: 1774 701
TatI w'GTAC_w
Cuts at: 0 1734 2475
Size: 1734 741
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 2475
Size: 1137 32 1306
Fragments arranged by size:
1306 1137 32
Tth111I GACn'n_nGTC
Cuts at: 0 277 2475
Size: 277 2198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1773 2194 2392 2475
Size: 1773 421 198 83
Fragments arranged by size:
1773 421 198 83
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AloI AlwNI ApaI
AvaI BaeI BanI BanII BcgI BciVI BglI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MfeI
MmeI MscI MslI MspA1I NheI NspI PasI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SanDI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BbsI BbvCI BclI BfrBI BglII BlpI BplI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PciI PflMI PmeI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI