Restriction map for
pFN21AE1896
(Linear) (Six-base) MAPSORT of: KIEE1896.seq Check: 2186 from: 1 to: 1281
KIEE1896.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 179 740 923 989 1136 1281
Size: 160 19 561 183 66 147 145
Fragments arranged by size:
561 183 160 147 145 66 19
AflII C'TTAA_G
Cuts at: 0 373 1281
Size: 373 908
AlwNI CAG_nnn'CTG
Cuts at: 0 1033 1281
Size: 1033 248
ApaLI G'TGCA_C
Cuts at: 0 860 1281
Size: 860 421
ApoI r'AATT_y
Cuts at: 0 20 391 476 1045 1281
Size: 20 371 85 569 236
Fragments arranged by size:
569 371 236 85 20
BanII G_rGCy'C
Cuts at: 0 221 1281
Size: 221 1060
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 443 1281
Size: 272 171 838
Fragments arranged by size:
838 272 171
BbvCI CC'TCA_GC
Cuts at: 0 332 1281
Size: 332 949
BclI T'GATC_A
Cuts at: 0 207 510 1281
Size: 207 303 771
Fragments arranged by size:
771 303 207
Bme1580I G_kGCm'C
Cuts at: 0 864 1281
Size: 864 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1281
Size: 635 646
Bpu10I CC'TnA_GC
Cuts at: 0 332 1076 1281
Size: 332 744 205
Fragments arranged by size:
744 332 205
BsaBI GATnn'nnATC
Cuts at: 0 341 1281
Size: 341 940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 608 638 1281
Size: 608 30 643
Fragments arranged by size:
643 608 30
BseYI C'CCAG_C
Cuts at: 0 731 1281
Size: 731 550
BsiHKAI G_wGCw'C
Cuts at: 0 864 1281
Size: 864 417
BsmI GAATG_Cn'
Cuts at: 0 657 1209 1281
Size: 657 552 72
Fragments arranged by size:
657 552 72
Bsp1286I G_dGCh'C
Cuts at: 0 221 864 1281
Size: 221 643 417
Fragments arranged by size:
643 417 221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 327 813 1281
Size: 327 486 468
Fragments arranged by size:
486 468 327
BstAPI GCAn_nnn'nTGC
Cuts at: 0 328 1281
Size: 328 953
BstYI r'GATC_y
Cuts at: 0 540 1281
Size: 540 741
DraI TTT'AAA
Cuts at: 0 951 1281
Size: 951 330
EarI CTCTTCn'nnn_
Cuts at: 0 40 965 1194 1281
Size: 40 925 229 87
Fragments arranged by size:
925 229 87 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 179 635 740 923 989 1136 1281
Size: 160 19 456 105 183 66 147 145
Fragments arranged by size:
456 183 160 147 145 105 66 19
EcoO109I rG'GnC_Cy
Cuts at: 0 230 1281
Size: 230 1051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1101 1281
Size: 1101 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1281
Size: 34 32 1215
Fragments arranged by size:
1215 34 32
HincII GTy'rAC
Cuts at: 0 533 1281
Size: 533 748
MfeI C'AATT_G
Cuts at: 0 1010 1281
Size: 1010 271
MspA1I CmG'CkG
Cuts at: 0 1096 1230 1281
Size: 1096 134 51
Fragments arranged by size:
1096 134 51
PfoI T'CCnGG_A
Cuts at: 0 461 1281
Size: 461 820
PstI C_TGCA'G
Cuts at: 0 451 1281
Size: 451 830
PvuII CAG'CTG
Cuts at: 0 1096 1230 1281
Size: 1096 134 51
Fragments arranged by size:
1096 134 51
ScaI AGT'ACT
Cuts at: 0 782 1281
Size: 782 499
SfcI C'TryA_G
Cuts at: 0 447 1276 1281
Size: 447 829 5
Fragments arranged by size:
829 447 5
SmlI C'TyrA_G
Cuts at: 0 373 1281
Size: 373 908
TatI w'GTAC_w
Cuts at: 0 780 886 1281
Size: 780 106 395
Fragments arranged by size:
780 395 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 555 587 608 640 1281
Size: 555 32 21 32 641
Fragments arranged by size:
641 555 32 32 21
XbaI T'CTAG_A
Cuts at: 0 367 1281
Size: 367 914
Enzymes that do cut:
AcuI AflII AlwNI ApaLI ApoI BanII BbsI BbvCI
BclI Bme1580I BpmI Bpu10I BsaBI BsaXI BseYI BsiHKAI
BsmI Bsp1286I BspMI BstAPI BstYI DraI EarI Eco57MI
EcoO109I FalI Hin4I HincII MfeI MspA1I PfoI PstI
PvuII ScaI SfcI SmlI TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII Tth111I
XcmI XhoI XmaI XmnI ZraI