Restriction map for
pFN21AE1892
(Linear) (Six-base) MAPSORT of: KIEE1892.seq Check: 5314 from: 1 to: 1263
KIEE1892.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1072 1263
Size: 1072 191
AccI GT'mk_AC
Cuts at: 0 688 1263
Size: 688 575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1246 1263
Size: 502 744 17
Fragments arranged by size:
744 502 17
AlwNI CAG_nnn'CTG
Cuts at: 0 44 939 1263
Size: 44 895 324
Fragments arranged by size:
895 324 44
ApaI G_GGCC'C
Cuts at: 0 27 1263
Size: 27 1236
ApoI r'AATT_y
Cuts at: 0 58 132 897 1126 1263
Size: 58 74 765 229 137
Fragments arranged by size:
765 229 137 74 58
AseI AT'TA_AT
Cuts at: 0 86 1263
Size: 86 1177
AvaI C'yCGr_G
Cuts at: 0 545 1263
Size: 545 718
BamHI G'GATC_C
Cuts at: 0 612 1263
Size: 612 651
BanII G_rGCy'C
Cuts at: 0 27 1263
Size: 27 1236
BbsI GAAGACnn'nnnn_
Cuts at: 0 724 1234 1263
Size: 724 510 29
Fragments arranged by size:
724 510 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 2 956 1263
Size: 2 954 307
Fragments arranged by size:
954 307 2
Bme1580I G_kGCm'C
Cuts at: 0 27 1042 1263
Size: 27 1015 221
Fragments arranged by size:
1015 221 27
BmgBI CAC'GTC
Cuts at: 0 1214 1263
Size: 1214 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 503 1113 1263
Size: 503 610 150
Fragments arranged by size:
610 503 150
BmtI G_CTAG'C
Cuts at: 0 1088 1263
Size: 1088 175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 1263
Size: 573 690
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 802 832 1263
Size: 802 30 431
Fragments arranged by size:
802 431 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 162 447 1222 1263
Size: 162 285 775 41
Fragments arranged by size:
775 285 162 41
BseYI C'CCAG_C
Cuts at: 0 1218 1263
Size: 1218 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1050 1263
Size: 904 146 213
Fragments arranged by size:
904 213 146
BsiEI CG_ry'CG
Cuts at: 0 469 514 518 1263
Size: 469 45 4 745
Fragments arranged by size:
745 469 45 4
BsmI GAATG_Cn'
Cuts at: 0 534 1132 1263
Size: 534 598 131
Fragments arranged by size:
598 534 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 95 1204 1263
Size: 95 1109 59
Fragments arranged by size:
1109 95 59
Bsp1286I G_dGCh'C
Cuts at: 0 27 1042 1263
Size: 27 1015 221
Fragments arranged by size:
1015 221 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 926 1072 1263
Size: 287 639 146 191
Fragments arranged by size:
639 287 191 146
BsrDI GCAATG_nn'
Cuts at: 0 404 1263
Size: 404 859
BsrFI r'CCGG_y
Cuts at: 0 464 513 1263
Size: 464 49 750
Fragments arranged by size:
750 464 49
BssSI C'ACGA_G
Cuts at: 0 391 1263
Size: 391 872
BstXI CCAn_nnnn'nTGG
Cuts at: 0 892 1023 1263
Size: 892 131 240
Fragments arranged by size:
892 240 131
BstYI r'GATC_y
Cuts at: 0 241 554 612 1263
Size: 241 313 58 651
Fragments arranged by size:
651 313 241 58
BstZ17I GTA'TAC
Cuts at: 0 689 1263
Size: 689 574
BtgI C'CryG_G
Cuts at: 0 831 1263
Size: 831 432
BtsI GCAGTG_nn'
Cuts at: 0 269 566 1199 1263
Size: 269 297 633 64
Fragments arranged by size:
633 297 269 64
DraI TTT'AAA
Cuts at: 0 228 1263
Size: 228 1035
EaeI y'GGCC_r
Cuts at: 0 48 466 511 515 665 1013 1263
Size: 48 418 45 4 150 348 250
Fragments arranged by size:
418 348 250 150 48 45 4
EagI C'GGCC_G
Cuts at: 0 466 511 515 1263
Size: 466 45 4 748
Fragments arranged by size:
748 466 45 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1246 1263
Size: 502 744 17
Fragments arranged by size:
744 502 17
EcoO109I rG'GnC_Cy
Cuts at: 0 23 24 276 285 972 1057 1160 1263
Size: 23 1 252 9 687 85 103 103
Fragments arranged by size:
687 252 103 103 85 23 9 1
EcoRI G'AATT_C
Cuts at: 0 132 1263
Size: 132 1131
FseI GG_CCGG'CC
Cuts at: 0 517 1263
Size: 517 746
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 539 571 850 882 1263
Size: 92 32 415 32 279 32 381
Fragments arranged by size:
415 381 279 92 32 32 32
HincII GTy'rAC
Cuts at: 0 124 1263
Size: 124 1139
MfeI C'AATT_G
Cuts at: 0 269 1263
Size: 269 994
MscI TGG'CCA
Cuts at: 0 50 667 1263
Size: 50 617 596
Fragments arranged by size:
617 596 50
MspA1I CmG'CkG
Cuts at: 0 936 1263
Size: 936 327
NaeI GCC'GGC
Cuts at: 0 515 1263
Size: 515 748
NcoI C'CATG_G
Cuts at: 0 831 1263
Size: 831 432
NdeI CA'TA_TG
Cuts at: 0 951 1263
Size: 951 312
NgoMIV G'CCGG_C
Cuts at: 0 513 1263
Size: 513 750
NheI G'CTAG_C
Cuts at: 0 1084 1263
Size: 1084 179
NspI r_CATG'y
Cuts at: 0 1246 1263
Size: 1246 17
PasI CC'CwG_GG
Cuts at: 0 246 1263
Size: 246 1017
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 396 1263
Size: 375 21 867
Fragments arranged by size:
867 375 21
PfoI T'CCnGG_A
Cuts at: 0 735 1263
Size: 735 528
PpuMI rG'GwC_Cy
Cuts at: 0 276 1057 1160 1263
Size: 276 781 103 103
Fragments arranged by size:
781 276 103 103
PspOMI G'GGCC_C
Cuts at: 0 23 1263
Size: 23 1240
PstI C_TGCA'G
Cuts at: 0 221 284 1206 1263
Size: 221 63 922 57
Fragments arranged by size:
922 221 63 57
PvuII CAG'CTG
Cuts at: 0 936 1263
Size: 936 327
SanDI GG'GwC_CC
Cuts at: 0 1057 1263
Size: 1057 206
SbfI CC_TGCA'GG
Cuts at: 0 284 1263
Size: 284 979
SfcI C'TryA_G
Cuts at: 0 217 280 1202 1263
Size: 217 63 922 61
Fragments arranged by size:
922 217 63 61
SpeI A'CTAG_T
Cuts at: 0 126 1263
Size: 126 1137
StyI C'CwwG_G
Cuts at: 0 831 1263
Size: 831 432
SwaI ATTT'AAAT
Cuts at: 0 228 1263
Size: 228 1035
TatI w'GTAC_w
Cuts at: 0 477 1263
Size: 477 786
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1062 1094 1263
Size: 1062 32 169
Fragments arranged by size:
1062 169 32
Tth111I GACn'n_nGTC
Cuts at: 0 723 1263
Size: 723 540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 770 852 1263
Size: 770 82 411
Fragments arranged by size:
770 411 82
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI AseI AvaI
BamHI BanII BbsI BciVI Bme1580I BmgBI BmrI BmtI
BplI BsaXI BseRI BseYI BsgI BsiEI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI BstZ17I
BtgI BtsI DraI EaeI EagI Eco57MI EcoO109I EcoRI
FseI Hin4I HincII MfeI MscI MspA1I NaeI NcoI
NdeI NgoMIV NheI NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SanDI SbfI SfcI SpeI StyI
SwaI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiHKAI BsiWI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII Bsu36I BtgZI ClaI CspCI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoRV FalI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI
MslI NarI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI
StuI TaqII XbaI XhoI XmaI XmnI ZraI