Restriction map for
pFN21AE1891
(Linear) (Six-base) MAPSORT of: KIEE1891.seq Check: 6688 from: 1 to: 1260
KIEE1891.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 39 1260
Size: 39 1221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1260
Size: 595 665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1147 1260
Size: 1147 113
AlwNI CAG_nnn'CTG
Cuts at: 0 257 1260
Size: 257 1003
ApoI r'AATT_y
Cuts at: 0 505 1260
Size: 505 755
AvaI C'yCGr_G
Cuts at: 0 226 295 845 1260
Size: 226 69 550 415
Fragments arranged by size:
550 415 226 69
BanI G'GyrC_C
Cuts at: 0 180 1044 1260
Size: 180 864 216
Fragments arranged by size:
864 216 180
BanII G_rGCy'C
Cuts at: 0 109 749 1260
Size: 109 640 511
Fragments arranged by size:
640 511 109
BbeI G_GCGC'C
Cuts at: 0 1048 1260
Size: 1048 212
BclI T'GATC_A
Cuts at: 0 927 1260
Size: 927 333
BglI GCCn_nnn'nGGC
Cuts at: 0 1180 1260
Size: 1180 80
BglII A'GATC_T
Cuts at: 0 682 1260
Size: 682 578
BlpI GC'TnA_GC
Cuts at: 0 310 1260
Size: 310 950
Bme1580I G_kGCm'C
Cuts at: 0 201 824 1260
Size: 201 623 436
Fragments arranged by size:
623 436 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 1260
Size: 103 1157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1111 1260
Size: 431 680 149
Fragments arranged by size:
680 431 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1257 1260
Size: 388 869 3
Fragments arranged by size:
869 388 3
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 1066 1260
Size: 9 1057 194
Fragments arranged by size:
1057 194 9
BsaHI Gr'CG_yC
Cuts at: 0 1045 1260
Size: 1045 215
BsaWI w'CCGG_w
Cuts at: 0 130 782 979 1260
Size: 130 652 197 281
Fragments arranged by size:
652 281 197 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 125 155 1260
Size: 125 30 1105
Fragments arranged by size:
1105 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 1260
Size: 244 1016
BseYI C'CCAG_C
Cuts at: 0 83 250 691 917 1260
Size: 83 167 441 226 343
Fragments arranged by size:
441 343 226 167 83
BsiEI CG_ry'CG
Cuts at: 0 40 1260
Size: 40 1220
BsiHKAI G_wGCw'C
Cuts at: 0 758 1117 1260
Size: 758 359 143
Fragments arranged by size:
758 359 143
BsmI GAATG_Cn'
Cuts at: 0 615 1260
Size: 615 645
Bsp1286I G_dGCh'C
Cuts at: 0 109 201 749 758 824 1117 1260
Size: 109 92 548 9 66 293 143
Fragments arranged by size:
548 293 143 109 92 66 9
BspEI T'CCGG_A
Cuts at: 0 130 782 1260
Size: 130 652 478
Fragments arranged by size:
652 478 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 839 1260
Size: 39 800 421
Fragments arranged by size:
800 421 39
BsrBI CCG'CTC
Cuts at: 0 225 1260
Size: 225 1035
BsrFI r'CCGG_y
Cuts at: 0 1047 1260
Size: 1047 213
BssHII G'CGCG_C
Cuts at: 0 153 1260
Size: 153 1107
BstAPI GCAn_nnn'nTGC
Cuts at: 0 621 1260
Size: 621 639
BstBI TT'CG_AA
Cuts at: 0 1199 1260
Size: 1199 61
BstYI r'GATC_y
Cuts at: 0 365 682 1260
Size: 365 317 578
Fragments arranged by size:
578 365 317
BtgI C'CryG_G
Cuts at: 0 912 1175 1260
Size: 912 263 85
Fragments arranged by size:
912 263 85
BtsI GCAGTG_nn'
Cuts at: 0 321 619 1260
Size: 321 298 641
Fragments arranged by size:
641 321 298
DrdI GACnn_nn'nnGTC
Cuts at: 0 847 1260
Size: 847 413
EaeI y'GGCC_r
Cuts at: 0 1106 1172 1260
Size: 1106 66 88
Fragments arranged by size:
1106 88 66
EarI CTCTTCn'nnn_
Cuts at: 0 257 956 1260
Size: 257 699 304
Fragments arranged by size:
699 304 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 117 1243 1260
Size: 117 1126 17
Fragments arranged by size:
1126 117 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 595 1111 1260
Size: 431 164 516 149
Fragments arranged by size:
516 431 164 149
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 1260
Size: 496 764
EcoO109I rG'GnC_Cy
Cuts at: 0 66 1260
Size: 66 1194
EcoRV GAT'ATC
Cuts at: 0 516 1260
Size: 516 744
HaeII r_GCGC'y
Cuts at: 0 10 84 97 1048 1260
Size: 10 74 13 951 212
Fragments arranged by size:
951 212 74 13 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 579 611 1260
Size: 223 32 324 32 649
Fragments arranged by size:
649 324 223 32 32
HindIII A'AGCT_T
Cuts at: 0 287 1260
Size: 287 973
KasI G'GCGC_C
Cuts at: 0 1044 1260
Size: 1044 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1260
Size: 554 706
MslI CAynn'nnrTG
Cuts at: 0 499 953 1260
Size: 499 454 307
Fragments arranged by size:
499 454 307
MspA1I CmG'CkG
Cuts at: 0 691 1054 1104 1215 1260
Size: 691 363 50 111 45
Fragments arranged by size:
691 363 111 50 45
NaeI GCC'GGC
Cuts at: 0 1049 1260
Size: 1049 211
NarI GG'CG_CC
Cuts at: 0 1045 1260
Size: 1045 215
NcoI C'CATG_G
Cuts at: 0 912 1175 1260
Size: 912 263 85
Fragments arranged by size:
912 263 85
NgoMIV G'CCGG_C
Cuts at: 0 1047 1260
Size: 1047 213
PasI CC'CwG_GG
Cuts at: 0 658 1260
Size: 658 602
PflMI CCAn_nnn'nTGG
Cuts at: 0 257 1260
Size: 257 1003
PpuMI rG'GwC_Cy
Cuts at: 0 66 1260
Size: 66 1194
PspXI vC'TCGA_Gb
Cuts at: 0 226 1260
Size: 226 1034
PstI C_TGCA'G
Cuts at: 0 50 690 706 1103 1260
Size: 50 640 16 397 157
Fragments arranged by size:
640 397 157 50 16
PvuII CAG'CTG
Cuts at: 0 691 1054 1104 1215 1260
Size: 691 363 50 111 45
Fragments arranged by size:
691 363 111 50 45
SfcI C'TryA_G
Cuts at: 0 46 686 702 1099 1260
Size: 46 640 16 397 161
Fragments arranged by size:
640 397 161 46 16
SfoI GGC'GCC
Cuts at: 0 1046 1260
Size: 1046 214
SmaI CCC'GGG
Cuts at: 0 297 1260
Size: 297 963
SmlI C'TyrA_G
Cuts at: 0 226 403 1236 1260
Size: 226 177 833 24
Fragments arranged by size:
833 226 177 24
StyI C'CwwG_G
Cuts at: 0 912 1175 1260
Size: 912 263 85
Fragments arranged by size:
912 263 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 909 1260
Size: 909 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 1260
Size: 125 32 1103
Fragments arranged by size:
1103 125 32
XbaI T'CTAG_A
Cuts at: 0 444 1260
Size: 444 816
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 729 1260
Size: 729 531
XhoI C'TCGA_G
Cuts at: 0 226 1260
Size: 226 1034
XmaI C'CCGG_G
Cuts at: 0 295 1260
Size: 295 965
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI BanI BanII
BbeI BclI BglI BglII BlpI Bme1580I BmrI BpmI
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstBI BstYI BtgI BtsI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HindIII
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PpuMI PspXI PstI PvuII SfcI SfoI
SmaI SmlI StyI TaqII TstI XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsgI BsiWI
BsmBI BspHI BsrDI BsrGI BssSI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI
EcoRI FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MscI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XmnI ZraI