Restriction map for
pFN21AE1890
(Linear) (Six-base) MAPSORT of: KIEE1890.seq Check: 4418 from: 1 to: 1254
KIEE1890.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1211 1254
Size: 1211 43
AccI GT'mk_AC
Cuts at: 0 239 1254
Size: 239 1015
AclI AA'CG_TT
Cuts at: 0 259 1254
Size: 259 995
AfeI AGC'GCT
Cuts at: 0 916 1254
Size: 916 338
AflIII A'CryG_T
Cuts at: 0 941 1254
Size: 941 313
AlwNI CAG_nnn'CTG
Cuts at: 0 1226 1254
Size: 1226 28
AvaI C'yCGr_G
Cuts at: 0 323 656 878 1069 1254
Size: 323 333 222 191 185
Fragments arranged by size:
333 323 222 191 185
AvrII C'CTAG_G
Cuts at: 0 825 1254
Size: 825 429
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 1254
Size: 365 889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 503 1254
Size: 469 34 751
Fragments arranged by size:
751 469 34
BclI T'GATC_A
Cuts at: 0 860 1254
Size: 860 394
BglI GCCn_nnn'nGGC
Cuts at: 0 851 1254
Size: 851 403
BglII A'GATC_T
Cuts at: 0 665 1254
Size: 665 589
BlpI GC'TnA_GC
Cuts at: 0 410 1254
Size: 410 844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1254
Size: 60 1194
BsaAI yAC'GTr
Cuts at: 0 753 804 1254
Size: 753 51 450
Fragments arranged by size:
753 450 51
BsaWI w'CCGG_w
Cuts at: 0 521 1127 1254
Size: 521 606 127
Fragments arranged by size:
606 521 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 793 823 1254
Size: 727 30 36 30 431
Fragments arranged by size:
727 431 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 489 649 735 801 970 1254
Size: 489 160 86 66 169 284
Fragments arranged by size:
489 284 169 160 86 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1239 1254
Size: 1239 15
BsiEI CG_ry'CG
Cuts at: 0 1250 1254
Size: 1250 4
BsiHKAI G_wGCw'C
Cuts at: 0 521 1254
Size: 521 733
BsmI GAATG_Cn'
Cuts at: 0 220 1254
Size: 220 1034
Bsp1286I G_dGCh'C
Cuts at: 0 521 1254
Size: 521 733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1190 1211 1254
Size: 1190 21 43
Fragments arranged by size:
1190 43 21
BsrDI GCAATG_nn'
Cuts at: 0 159 1254
Size: 159 1095
BsrFI r'CCGG_y
Cuts at: 0 692 1254
Size: 692 562
BssSI C'ACGA_G
Cuts at: 0 952 1095 1254
Size: 952 143 159
Fragments arranged by size:
952 159 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1031 1254
Size: 1031 223
BstYI r'GATC_y
Cuts at: 0 665 1254
Size: 665 589
BtgI C'CryG_G
Cuts at: 0 177 846 1254
Size: 177 669 408
Fragments arranged by size:
669 408 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 434 1254
Size: 434 820
BtsI GCAGTG_nn'
Cuts at: 0 972 1203 1254
Size: 972 231 51
Fragments arranged by size:
972 231 51
EaeI y'GGCC_r
Cuts at: 0 1247 1254
Size: 1247 7
EagI C'GGCC_G
Cuts at: 0 1247 1254
Size: 1247 7
EarI CTCTTCn'nnn_
Cuts at: 0 466 1254
Size: 466 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 912 1000 1254
Size: 912 88 254
Fragments arranged by size:
912 254 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1254
Size: 60 1194
EcoNI CCTnn'n_nnAGG
Cuts at: 0 333 829 1254
Size: 333 496 425
Fragments arranged by size:
496 425 333
EcoO109I rG'GnC_Cy
Cuts at: 0 599 774 834 1254
Size: 599 175 60 420
Fragments arranged by size:
599 420 175 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 430 1254
Size: 430 824
FspI TGC'GCA
Cuts at: 0 646 1254
Size: 646 608
FspAI rTGC'GCAy
Cuts at: 0 646 1254
Size: 646 608
HaeII r_GCGC'y
Cuts at: 0 918 1254
Size: 918 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 727 759 1078 1110 1254
Size: 95 32 600 32 319 32 144
Fragments arranged by size:
600 319 144 95 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1043 1254
Size: 280 763 211
Fragments arranged by size:
763 280 211
MspA1I CmG'CkG
Cuts at: 0 80 848 1254
Size: 80 768 406
Fragments arranged by size:
768 406 80
NcoI C'CATG_G
Cuts at: 0 177 1254
Size: 177 1077
NotI GC'GGCC_GC
Cuts at: 0 1247 1254
Size: 1247 7
NspI r_CATG'y
Cuts at: 0 945 1029 1047 1254
Size: 945 84 18 207
Fragments arranged by size:
945 207 84 18
PasI CC'CwG_GG
Cuts at: 0 537 1254
Size: 537 717
PciI A'CATG_T
Cuts at: 0 941 1254
Size: 941 313
PfoI T'CCnGG_A
Cuts at: 0 620 1254
Size: 620 634
PpuMI rG'GwC_Cy
Cuts at: 0 774 834 1254
Size: 774 60 420
Fragments arranged by size:
774 420 60
PstI C_TGCA'G
Cuts at: 0 937 1254
Size: 937 317
SacII CC_GC'GG
Cuts at: 0 849 1254
Size: 849 405
SfcI C'TryA_G
Cuts at: 0 443 933 1254
Size: 443 490 321
Fragments arranged by size:
490 443 321
SmaI CCC'GGG
Cuts at: 0 658 880 1254
Size: 658 222 374
Fragments arranged by size:
658 374 222
SphI G_CATG'C
Cuts at: 0 1047 1254
Size: 1047 207
StyI C'CwwG_G
Cuts at: 0 177 595 764 825 1254
Size: 177 418 169 61 429
Fragments arranged by size:
429 418 177 169 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 32 1254
Size: 19 13 1222
Fragments arranged by size:
1222 19 13
TatI w'GTAC_w
Cuts at: 0 929 1254
Size: 929 325
Tth111I GACn'n_nGTC
Cuts at: 0 235 1254
Size: 235 1019
XmaI C'CCGG_G
Cuts at: 0 656 878 1254
Size: 656 222 376
Fragments arranged by size:
656 376 222
Enzymes that do cut:
AarI AccI AclI AfeI AflIII AlwNI AvaI AvrII
BbsI BcgI BclI BglI BglII BlpI BpmI BsaAI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrFI BssSI BstAPI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI FspI FspAI HaeII Hin4I MmeI MspA1I NcoI
NotI NspI PasI PciI PfoI PpuMI PstI SacII
SfcI SmaI SphI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AcuI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BfrBI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BseYI BsiWI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI XmnI ZraI