Restriction map for
pFN21AE1885
(Linear) (Six-base) MAPSORT of: KIEE1885.seq Check: 7356 from: 1 to: 1233
KIEE1885.orf
With 182 enzymes: SgfI *
March 22, 2012 15:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1233
Size: 77 1156
AfeI AGC'GCT
Cuts at: 0 162 1233
Size: 162 1071
AhdI GACnn_n'nnGTC
Cuts at: 0 1002 1233
Size: 1002 231
AleI CACnn'nnGTG
Cuts at: 0 937 1233
Size: 937 296
ApaLI G'TGCA_C
Cuts at: 0 526 1233
Size: 526 707
AvaI C'yCGr_G
Cuts at: 0 97 193 988 1163 1233
Size: 97 96 795 175 70
Fragments arranged by size:
795 175 97 96 70
BamHI G'GATC_C
Cuts at: 0 863 1233
Size: 863 370
BanI G'GyrC_C
Cuts at: 0 548 1121 1233
Size: 548 573 112
Fragments arranged by size:
573 548 112
BanII G_rGCy'C
Cuts at: 0 174 299 1233
Size: 174 125 934
Fragments arranged by size:
934 174 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1062 1233
Size: 1062 171
BclI T'GATC_A
Cuts at: 0 830 1233
Size: 830 403
BglI GCCn_nnn'nGGC
Cuts at: 0 394 1233
Size: 394 839
BlpI GC'TnA_GC
Cuts at: 0 1101 1233
Size: 1101 132
Bme1580I G_kGCm'C
Cuts at: 0 193 530 1233
Size: 193 337 703
Fragments arranged by size:
703 337 193
BmgBI CAC'GTC
Cuts at: 0 263 1233
Size: 263 970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1233
Size: 1116 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1233
Size: 49 1184
BsaHI Gr'CG_yC
Cuts at: 0 209 1233
Size: 209 1024
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 161 172 191 202 1126 1156 1233
Size: 161 11 19 11 924 30 77
Fragments arranged by size:
924 161 77 30 19 11 11
BseYI C'CCAG_C
Cuts at: 0 77 1233
Size: 77 1156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 610 1233
Size: 316 294 623
Fragments arranged by size:
623 316 294
BsiEI CG_ry'CG
Cuts at: 0 1226 1233
Size: 1226 7
BsiHKAI G_wGCw'C
Cuts at: 0 530 827 944 1233
Size: 530 297 117 289
Fragments arranged by size:
530 297 289 117
BsmI GAATG_Cn'
Cuts at: 0 237 250 428 1233
Size: 237 13 178 805
Fragments arranged by size:
805 237 178 13
Bsp1286I G_dGCh'C
Cuts at: 0 174 193 299 530 827 944 1233
Size: 174 19 106 231 297 117 289
Fragments arranged by size:
297 289 231 174 117 106 19
BspHI T'CATG_A
Cuts at: 0 641 1233
Size: 641 592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 632 1233
Size: 338 294 601
Fragments arranged by size:
601 338 294
BsrDI GCAATG_nn'
Cuts at: 0 543 1233
Size: 543 690
BssSI C'ACGA_G
Cuts at: 0 175 529 820 1233
Size: 175 354 291 413
Fragments arranged by size:
413 354 291 175
BstEII G'GTnAC_C
Cuts at: 0 585 1233
Size: 585 648
BstYI r'GATC_y
Cuts at: 0 863 1233
Size: 863 370
BtgI C'CryG_G
Cuts at: 0 1157 1233
Size: 1157 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 1233
Size: 327 906
BtsI GCAGTG_nn'
Cuts at: 0 10 369 929 1233
Size: 10 359 560 304
Fragments arranged by size:
560 359 304 10
DraIII CAC_nnn'GTG
Cuts at: 0 292 1233
Size: 292 941
EaeI y'GGCC_r
Cuts at: 0 395 632 1223 1233
Size: 395 237 591 10
Fragments arranged by size:
591 395 237 10
EagI C'GGCC_G
Cuts at: 0 1223 1233
Size: 1223 10
EarI CTCTTCn'nnn_
Cuts at: 0 53 682 688 979 1108 1233
Size: 53 629 6 291 129 125
Fragments arranged by size:
629 291 129 125 53 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 77 1233
Size: 49 28 1156
Fragments arranged by size:
1156 49 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 1233
Size: 28 1205
EcoO109I rG'GnC_Cy
Cuts at: 0 103 808 1233
Size: 103 705 425
Fragments arranged by size:
705 425 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 1233
Size: 693 540
FspI TGC'GCA
Cuts at: 0 667 1233
Size: 667 566
HaeII r_GCGC'y
Cuts at: 0 52 164 663 1233
Size: 52 112 499 570
Fragments arranged by size:
570 499 112 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 683 715 988 1020 1126 1158 1233
Size: 683 32 273 32 106 32 75
Fragments arranged by size:
683 273 106 75 32 32 32
HincII GTy'rAC
Cuts at: 0 357 516 1233
Size: 357 159 717
Fragments arranged by size:
717 357 159
MslI CAynn'nnrTG
Cuts at: 0 640 820 937 967 1233
Size: 640 180 117 30 266
Fragments arranged by size:
640 266 180 117 30
MspA1I CmG'CkG
Cuts at: 0 554 1127 1228 1233
Size: 554 573 101 5
Fragments arranged by size:
573 554 101 5
NcoI C'CATG_G
Cuts at: 0 1157 1233
Size: 1157 76
NspI r_CATG'y
Cuts at: 0 972 1233
Size: 972 261
PpuMI rG'GwC_Cy
Cuts at: 0 103 1233
Size: 103 1130
PstI C_TGCA'G
Cuts at: 0 43 509 1043 1233
Size: 43 466 534 190
Fragments arranged by size:
534 466 190 43
SanDI GG'GwC_CC
Cuts at: 0 103 1233
Size: 103 1130
SapI GCTCTTCn'nnn_
Cuts at: 0 53 979 1233
Size: 53 926 254
Fragments arranged by size:
926 254 53
ScaI AGT'ACT
Cuts at: 0 199 1233
Size: 199 1034
SexAI A'CCwGG_T
Cuts at: 0 785 1233
Size: 785 448
SfcI C'TryA_G
Cuts at: 0 39 505 1039 1233
Size: 39 466 534 194
Fragments arranged by size:
534 466 194 39
SmaI CCC'GGG
Cuts at: 0 990 1165 1233
Size: 990 175 68
Fragments arranged by size:
990 175 68
SrfI GCCC'GGGC
Cuts at: 0 1165 1233
Size: 1165 68
StyI C'CwwG_G
Cuts at: 0 128 804 1157 1233
Size: 128 676 353 76
Fragments arranged by size:
676 353 128 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1203 1233
Size: 1203 30
TatI w'GTAC_w
Cuts at: 0 197 215 911 1233
Size: 197 18 696 322
Fragments arranged by size:
696 322 197 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 161 172 193 204 1233
Size: 161 11 21 11 1029
Fragments arranged by size:
1029 161 21 11 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 1233
Size: 42 1191
XmaI C'CCGG_G
Cuts at: 0 988 1163 1233
Size: 988 175 70
Fragments arranged by size:
988 175 70
Enzymes that do cut:
AcuI AfeI AhdI AleI ApaLI AvaI BamHI BanI
BanII BbsI BclI BglI BlpI Bme1580I BmgBI BplI
BpmI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BssSI BstEII BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII MslI MspA1I
NcoI NspI PpuMI PstI SanDI SapI ScaI SexAI
SfcI SmaI SrfI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII
BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EciI EcoICRI EcoRI EcoRV FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SbfI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI
XhoI XmnI ZraI