(Linear) (Six-base) MAPSORT of: KIEE1884.seq  Check: 9935  from: 1  to: 1230



KIEE1884.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:49  ..

AccI GT'mk_AC

Cuts at:      0     323    1230 
   Size:        323     907
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     101     745     895    1230 
   Size:         16      85     644     150     335

  Fragments arranged by size: 

                 644     335     150      85      16
 
AflII C'TTAA_G

Cuts at:      0     882    1230 
   Size:        882     348
 
AlwNI CAG_nnn'CTG

Cuts at:      0     145     349    1230 
   Size:        145     204     881

  Fragments arranged by size: 

                 881     204     145
 
ApoI r'AATT_y

Cuts at:      0     619     707     751    1172    1230 
   Size:        619      88      44     421      58

  Fragments arranged by size: 

                 619     421      88      58      44
 
AvaI C'yCGr_G

Cuts at:      0     420     839    1230 
   Size:        420     419     391

  Fragments arranged by size: 

                 420     419     391
 
BanII G_rGCy'C

Cuts at:      0     503     846    1230 
   Size:        503     343     384

  Fragments arranged by size: 

                 503     384     343
 
BclI T'GATC_A

Cuts at:      0     531    1230 
   Size:        531     699
 
BglII A'GATC_T

Cuts at:      0     603    1230 
   Size:        603     627
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1149    1230 
   Size:       1149      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     594    1230 
   Size:        594     636
 
Bpu10I CC'TnA_GC

Cuts at:      0     149    1230 
   Size:        149    1081
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26    1230 
   Size:         26    1204
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     722     752    1230 
   Size:        722      30     478

  Fragments arranged by size: 

                 722     478      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1124    1230 
   Size:       1124     106
 
BseYI C'CCAG_C

Cuts at:      0     138    1230 
   Size:        138    1092
 
BsiHKAI G_wGCw'C

Cuts at:      0     831    1230 
   Size:        831     399
 
BsmI GAATG_Cn'

Cuts at:      0    1130    1230 
   Size:       1130     100
 
Bsp1286I G_dGCh'C

Cuts at:      0     503     831     846    1230 
   Size:        503     328      15     384

  Fragments arranged by size: 

                 503     384     328      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     826    1230 
   Size:        826     404
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     576    1230 
   Size:        576     654
 
BstYI r'GATC_y

Cuts at:      0     603    1230 
   Size:        603     627
 
BtsI GCAGTG_nn'

Cuts at:      0     686    1062    1150    1230 
   Size:        686     376      88      80

  Fragments arranged by size: 

                 686     376      88      80
 
DraI TTT'AAA

Cuts at:      0     306    1230 
   Size:        306     924
 
EarI CTCTTCn'nnn_

Cuts at:      0    1080    1230 
   Size:       1080     150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     101     594     745     895    1230 
   Size:         16      85     493     151     150     335

  Fragments arranged by size: 

                 493     335     151     150      85      16
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     370    1230 
   Size:        370     860
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     351     375     383     407    1230 
   Size:        351      24       8      24     823

  Fragments arranged by size: 

                 823     351      24      24       8
 
HindIII A'AGCT_T

Cuts at:      0      44    1230 
   Size:         44    1186
 
MslI CAynn'nnrTG

Cuts at:      0     527    1230 
   Size:        527     703
 
MspA1I CmG'CkG

Cuts at:      0     516    1230 
   Size:        516     714
 
NdeI CA'TA_TG

Cuts at:      0     524    1023    1230 
   Size:        524     499     207

  Fragments arranged by size: 

                 524     499     207
 
PspXI vC'TCGA_Gb

Cuts at:      0     839    1230 
   Size:        839     391
 
PvuII CAG'CTG

Cuts at:      0     516    1230 
   Size:        516     714
 
SfcI C'TryA_G

Cuts at:      0     949    1230 
   Size:        949     281
 
SmaI CCC'GGG

Cuts at:      0     422    1230 
   Size:        422     808
 
SmlI C'TyrA_G

Cuts at:      0      41     839     882    1230 
   Size:         41     798      43     348

  Fragments arranged by size: 

                 798     348      43      41
 
TatI w'GTAC_w

Cuts at:      0     645    1230 
   Size:        645     585
 
Tth111I GACn'n_nGTC

Cuts at:      0     400    1155    1230 
   Size:        400     755      75

  Fragments arranged by size: 

                 755     400      75
 
XhoI C'TCGA_G

Cuts at:      0     839    1230 
   Size:        839     391
 
XmaI C'CCGG_G

Cuts at:      0     420    1230 
   Size:        420     810
 
XmnI GAAnn'nnTTC

Cuts at:      0     128    1230 
   Size:        128    1102
 

 Enzymes that do cut:

     AccI     AcuI    AflII    AlwNI     ApoI     AvaI    BanII     BclI
    BglII     BmrI     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I   BstAPI    BstXI    BstYI     BtsI     DraI
     EarI  Eco57MI     FalI    Hin4I  HindIII     MslI   MspA1I     NdeI
    PspXI    PvuII     SfcI     SmaI     SmlI     TatI  Tth111I     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI     XbaI
     XcmI     ZraI