Restriction map for
pFN21AE1883
(Linear) (Six-base) MAPSORT of: KIEE1883.seq Check: 9250 from: 1 to: 1227
KIEE1883.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 894 1227
Size: 894 333
AccI GT'mk_AC
Cuts at: 0 724 1151 1227
Size: 724 427 76
Fragments arranged by size:
724 427 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1079 1227
Size: 214 865 148
Fragments arranged by size:
865 214 148
AflIII A'CryG_T
Cuts at: 0 980 1227
Size: 980 247
AhdI GACnn_n'nnGTC
Cuts at: 0 12 1227
Size: 12 1215
AleI CACnn'nnGTG
Cuts at: 0 655 1227
Size: 655 572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 808 904 1227
Size: 808 96 323
Fragments arranged by size:
808 323 96
AlwNI CAG_nnn'CTG
Cuts at: 0 478 1195 1227
Size: 478 717 32
Fragments arranged by size:
717 478 32
ApoI r'AATT_y
Cuts at: 0 1002 1227
Size: 1002 225
AvaI C'yCGr_G
Cuts at: 0 115 631 1227
Size: 115 516 596
Fragments arranged by size:
596 516 115
BamHI G'GATC_C
Cuts at: 0 140 468 1227
Size: 140 328 759
Fragments arranged by size:
759 328 140
BanI G'GyrC_C
Cuts at: 0 888 1227
Size: 888 339
BanII G_rGCy'C
Cuts at: 0 820 1227
Size: 820 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1112 1146 1227
Size: 1112 34 81
Fragments arranged by size:
1112 81 34
BfrBI ATG'CAT
Cuts at: 0 499 1227
Size: 499 728
BglII A'GATC_T
Cuts at: 0 549 1227
Size: 549 678
Bme1580I G_kGCm'C
Cuts at: 0 735 893 1227
Size: 735 158 334
Fragments arranged by size:
735 334 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 839 1227
Size: 839 388
BsaBI GATnn'nnATC
Cuts at: 0 880 1227
Size: 880 347
BsaWI w'CCGG_w
Cuts at: 0 449 713 1227
Size: 449 264 514
Fragments arranged by size:
514 449 264
BseYI C'CCAG_C
Cuts at: 0 234 698 1227
Size: 234 464 529
Fragments arranged by size:
529 464 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1227
Size: 922 305
BsiHKAI G_wGCw'C
Cuts at: 0 569 820 1227
Size: 569 251 407
Fragments arranged by size:
569 407 251
BsmI GAATG_Cn'
Cuts at: 0 393 1227
Size: 393 834
BsmBI CGTCTCn'nnnn_
Cuts at: 0 339 1227
Size: 339 888
Bsp1286I G_dGCh'C
Cuts at: 0 569 735 820 893 1227
Size: 569 166 85 73 334
Fragments arranged by size:
569 334 166 85 73
BspEI T'CCGG_A
Cuts at: 0 449 1227
Size: 449 778
BspHI T'CATG_A
Cuts at: 0 177 1227
Size: 177 1050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2 894 1227
Size: 2 892 333
Fragments arranged by size:
892 333 2
BsrDI GCAATG_nn'
Cuts at: 0 1118 1227
Size: 1118 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1006 1108 1227
Size: 1006 102 119
Fragments arranged by size:
1006 119 102
BstYI r'GATC_y
Cuts at: 0 140 468 549 1227
Size: 140 328 81 678
Fragments arranged by size:
678 328 140 81
BtgI C'CryG_G
Cuts at: 0 201 269 774 1227
Size: 201 68 505 453
Fragments arranged by size:
505 453 201 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1115 1227
Size: 1115 112
BtsI GCAGTG_nn'
Cuts at: 0 599 802 1227
Size: 599 203 425
Fragments arranged by size:
599 425 203
EarI CTCTTCn'nnn_
Cuts at: 0 61 1227
Size: 61 1166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 1227
Size: 256 971
EcoICRI GAG'CTC
Cuts at: 0 818 1227
Size: 818 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1079 1227
Size: 214 865 148
Fragments arranged by size:
865 214 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 460 492 519 551 566 598 658
Size: 30 430 32 27 32 15 32 60
Cuts at: 658 690 1227
Size: 32 537
Fragments arranged by size:
537 430 60 32 32 32 32 30
27 15
HincII GTy'rAC
Cuts at: 0 725 1227
Size: 725 502
HindIII A'AGCT_T
Cuts at: 0 1091 1227
Size: 1091 136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 651 1200 1227
Size: 155 496 549 27
Fragments arranged by size:
549 496 155 27
MslI CAynn'nnrTG
Cuts at: 0 274 655 790 886 1227
Size: 274 381 135 96 341
Fragments arranged by size:
381 341 274 135 96
MspA1I CmG'CkG
Cuts at: 0 234 828 872 928 957 1227
Size: 234 594 44 56 29 270
Fragments arranged by size:
594 270 234 56 44 29
NcoI C'CATG_G
Cuts at: 0 201 269 1227
Size: 201 68 958
Fragments arranged by size:
958 201 68
NsiI A_TGCA'T
Cuts at: 0 501 1227
Size: 501 726
NspI r_CATG'y
Cuts at: 0 795 984 1227
Size: 795 189 243
Fragments arranged by size:
795 243 189
PciI A'CATG_T
Cuts at: 0 980 1227
Size: 980 247
PflMI CCAn_nnn'nTGG
Cuts at: 0 41 1227
Size: 41 1186
PstI C_TGCA'G
Cuts at: 0 13 1227
Size: 13 1214
PvuII CAG'CTG
Cuts at: 0 234 872 928 1227
Size: 234 638 56 299
Fragments arranged by size:
638 299 234 56
SacI G_AGCT'C
Cuts at: 0 820 1227
Size: 820 407
SalI G'TCGA_C
Cuts at: 0 723 1227
Size: 723 504
SfcI C'TryA_G
Cuts at: 0 9 929 1187 1227
Size: 9 920 258 40
Fragments arranged by size:
920 258 40 9
SmlI C'TyrA_G
Cuts at: 0 115 1227
Size: 115 1112
SphI G_CATG'C
Cuts at: 0 795 1227
Size: 795 432
SspI AAT'ATT
Cuts at: 0 308 1227
Size: 308 919
StuI AGG'CCT
Cuts at: 0 174 1227
Size: 174 1053
StyI C'CwwG_G
Cuts at: 0 20 201 269 1227
Size: 20 181 68 958
Fragments arranged by size:
958 181 68 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 1227
Size: 204 1023
XhoI C'TCGA_G
Cuts at: 0 115 1227
Size: 115 1112
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApoI AvaI BamHI BanI BanII BcgI BfrBI BglII
Bme1580I BplI BsaBI BsaWI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstYI
BtgI BtgZI BtsI EarI EciI EcoICRI Eco57MI Hin4I
HincII HindIII MmeI MslI MspA1I NcoI NsiI NspI
PciI PflMI PstI PvuII SacI SalI SfcI SmlI
SphI SspI StuI StyI TaqII XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BciVI BclI BglI BlpI BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BsiEI BsiWI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI