Restriction map for
pFN21AE1882
(Linear) (Six-base) MAPSORT of: KIEE1882.seq Check: 1163 from: 1 to: 1203
KIEE1882.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 753 1203
Size: 753 450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 454 1203
Size: 365 89 749
Fragments arranged by size:
749 365 89
AfeI AGC'GCT
Cuts at: 0 952 1203
Size: 952 251
AleI CACnn'nnGTG
Cuts at: 0 124 1203
Size: 124 1079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 1094 1203
Size: 464 630 109
Fragments arranged by size:
630 464 109
AlwNI CAG_nnn'CTG
Cuts at: 0 444 484 1047 1203
Size: 444 40 563 156
Fragments arranged by size:
563 444 156 40
ApaLI G'TGCA_C
Cuts at: 0 925 1203
Size: 925 278
BanI G'GyrC_C
Cuts at: 0 511 1203
Size: 511 692
BanII G_rGCy'C
Cuts at: 0 961 1203
Size: 961 242
BbeI G_GCGC'C
Cuts at: 0 515 1203
Size: 515 688
BciVI GTATCCnnnnn_n'
Cuts at: 0 1053 1203
Size: 1053 150
BclI T'GATC_A
Cuts at: 0 84 720 1203
Size: 84 636 483
Fragments arranged by size:
636 483 84
BlpI GC'TnA_GC
Cuts at: 0 521 1203
Size: 521 682
Bme1580I G_kGCm'C
Cuts at: 0 929 1203
Size: 929 274
BmrI ACTGGGnnnn_n'
Cuts at: 0 596 1203
Size: 596 607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 784 1203
Size: 784 419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 481 721 975 1203
Size: 269 212 240 254 228
Fragments arranged by size:
269 254 240 228 212
Bpu10I CC'TnA_GC
Cuts at: 0 638 980 1203
Size: 638 342 223
Fragments arranged by size:
638 342 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1203
Size: 641 562
BsaBI GATnn'nnATC
Cuts at: 0 243 1203
Size: 243 960
BsaHI Gr'CG_yC
Cuts at: 0 512 1203
Size: 512 691
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 403 1203
Size: 121 282 800
Fragments arranged by size:
800 282 121
BseYI C'CCAG_C
Cuts at: 0 296 471 1067 1194 1203
Size: 296 175 596 127 9
Fragments arranged by size:
596 296 175 127 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1009 1141 1203
Size: 1000 9 132 62
Fragments arranged by size:
1000 132 62 9
BsiHKAI G_wGCw'C
Cuts at: 0 929 1203
Size: 929 274
Bsp1286I G_dGCh'C
Cuts at: 0 929 961 1203
Size: 929 32 242
Fragments arranged by size:
929 242 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 739 1203
Size: 739 464
BsrDI GCAATG_nn'
Cuts at: 0 32 962 1203
Size: 32 930 241
Fragments arranged by size:
930 241 32
BssSI C'ACGA_G
Cuts at: 0 1018 1203
Size: 1018 185
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1203
Size: 1029 174
Bsu36I CC'TnA_GG
Cuts at: 0 77 1203
Size: 77 1126
EaeI y'GGCC_r
Cuts at: 0 1145 1203
Size: 1145 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1203
Size: 19 1184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 365 454 481 721 975 1203
Size: 269 96 89 27 240 254 228
Fragments arranged by size:
269 254 240 228 96 89 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 76 862 1203
Size: 76 786 341
Fragments arranged by size:
786 341 76
EcoO109I rG'GnC_Cy
Cuts at: 0 590 1203
Size: 590 613
HaeII r_GCGC'y
Cuts at: 0 315 515 528 690 809 954 1203
Size: 315 200 13 162 119 145 249
Fragments arranged by size:
315 249 200 162 145 119 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 1203
Size: 637 32 534
Fragments arranged by size:
637 534 32
KasI G'GCGC_C
Cuts at: 0 511 1203
Size: 511 692
MscI TGG'CCA
Cuts at: 0 1147 1203
Size: 1147 56
MslI CAynn'nnrTG
Cuts at: 0 124 1203
Size: 124 1079
MspA1I CmG'CkG
Cuts at: 0 423 444 540 709 1014 1203
Size: 423 21 96 169 305 189
Fragments arranged by size:
423 305 189 169 96 21
NarI GG'CG_CC
Cuts at: 0 512 1203
Size: 512 691
PpuMI rG'GwC_Cy
Cuts at: 0 590 1203
Size: 590 613
PstI C_TGCA'G
Cuts at: 0 539 750 1203
Size: 539 211 453
Fragments arranged by size:
539 453 211
PvuII CAG'CTG
Cuts at: 0 423 444 540 709 1014 1203
Size: 423 21 96 169 305 189
Fragments arranged by size:
423 305 189 169 96 21
SanDI GG'GwC_CC
Cuts at: 0 590 1203
Size: 590 613
SfcI C'TryA_G
Cuts at: 0 535 746 754 779 847 896 1203
Size: 535 211 8 25 68 49 307
Fragments arranged by size:
535 307 211 68 49 25 8
SfoI GGC'GCC
Cuts at: 0 513 1203
Size: 513 690
SmlI C'TyrA_G
Cuts at: 0 656 1203
Size: 656 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1081 1203
Size: 1081 122
Enzymes that do cut:
AccI AcuI AfeI AleI AlfI AlwNI ApaLI BanI
BanII BbeI BciVI BclI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI Bsu36I EaeI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NarI PpuMI PstI PvuII SanDI SfcI
SfoI SmlI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BcgI BfrBI BglI BglII
BmgBI BmtI BsaI BsaAI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI