Restriction map for
pFN21AE1880
(Linear) (Six-base) MAPSORT of: KIEE1880.seq Check: 6049 from: 1 to: 1218
KIEE1880.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1057 1218
Size: 1057 161
AccI GT'mk_AC
Cuts at: 0 194 1218
Size: 194 1024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1218
Size: 907 311
AflII C'TTAA_G
Cuts at: 0 1122 1218
Size: 1122 96
ApoI r'AATT_y
Cuts at: 0 871 1218
Size: 871 347
AvaI C'yCGr_G
Cuts at: 0 109 286 1218
Size: 109 177 932
Fragments arranged by size:
932 177 109
BamHI G'GATC_C
Cuts at: 0 482 1218
Size: 482 736
BanI G'GyrC_C
Cuts at: 0 562 1218
Size: 562 656
BanII G_rGCy'C
Cuts at: 0 746 979 1218
Size: 746 233 239
Fragments arranged by size:
746 239 233
BbsI GAAGACnn'nnnn_
Cuts at: 0 970 1049 1207 1218
Size: 970 79 158 11
Fragments arranged by size:
970 158 79 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 158 301 335 1218
Size: 124 34 143 34 883
Fragments arranged by size:
883 143 124 34 34
BclI T'GATC_A
Cuts at: 0 572 1218
Size: 572 646
BfrBI ATG'CAT
Cuts at: 0 926 1218
Size: 926 292
Bme1580I G_kGCm'C
Cuts at: 0 9 1218
Size: 9 1209
BmrI ACTGGGnnnn_n'
Cuts at: 0 466 1124 1200 1218
Size: 466 658 76 18
Fragments arranged by size:
658 466 76 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1218
Size: 1111 107
BsaBI GATnn'nnATC
Cuts at: 0 790 1218
Size: 790 428
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 176 206 353 383 1218
Size: 176 30 147 30 835
Fragments arranged by size:
835 176 147 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1108 1218
Size: 1108 110
BseYI C'CCAG_C
Cuts at: 0 848 1218
Size: 848 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1009 1218
Size: 496 513 209
Fragments arranged by size:
513 496 209
BsiHKAI G_wGCw'C
Cuts at: 0 137 314 1218
Size: 137 177 904
Fragments arranged by size:
904 177 137
Bsp1286I G_dGCh'C
Cuts at: 0 9 137 314 746 979 1218
Size: 9 128 177 432 233 239
Fragments arranged by size:
432 239 233 177 128 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1057 1218
Size: 1057 161
BsrDI GCAATG_nn'
Cuts at: 0 20 72 647 1218
Size: 20 52 575 571
Fragments arranged by size:
575 571 52 20
BsrGI T'GTAC_A
Cuts at: 0 371 1218
Size: 371 847
BstYI r'GATC_y
Cuts at: 0 482 1118 1218
Size: 482 636 100
Fragments arranged by size:
636 482 100
BstZ17I GTA'TAC
Cuts at: 0 195 1218
Size: 195 1023
BtgI C'CryG_G
Cuts at: 0 596 1218
Size: 596 622
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1073 1218
Size: 1073 145
BtsI GCAGTG_nn'
Cuts at: 0 59 223 400 1074 1218
Size: 59 164 177 674 144
Fragments arranged by size:
674 177 164 144 59
DraI TTT'AAA
Cuts at: 0 870 1218
Size: 870 348
EarI CTCTTCn'nnn_
Cuts at: 0 616 967 1218
Size: 616 351 251
Fragments arranged by size:
616 351 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1111 1218
Size: 907 204 107
Fragments arranged by size:
907 204 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1093 1218
Size: 1093 125
EcoRV GAT'ATC
Cuts at: 0 81 1218
Size: 81 1137
FspI TGC'GCA
Cuts at: 0 175 352 1218
Size: 175 177 866
Fragments arranged by size:
866 177 175
HindIII A'AGCT_T
Cuts at: 0 1125 1218
Size: 1125 93
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 393 1218
Size: 216 177 825
Fragments arranged by size:
825 216 177
MslI CAynn'nnrTG
Cuts at: 0 125 302 500 1218
Size: 125 177 198 718
Fragments arranged by size:
718 198 177 125
MspA1I CmG'CkG
Cuts at: 0 480 1218
Size: 480 738
NsiI A_TGCA'T
Cuts at: 0 928 1218
Size: 928 290
NspI r_CATG'y
Cuts at: 0 1029 1218
Size: 1029 189
PasI CC'CwG_GG
Cuts at: 0 9 1218
Size: 9 1209
PfoI T'CCnGG_A
Cuts at: 0 1184 1218
Size: 1184 34
SapI GCTCTTCn'nnn_
Cuts at: 0 967 1218
Size: 967 251
SexAI A'CCwGG_T
Cuts at: 0 1102 1218
Size: 1102 116
SfcI C'TryA_G
Cuts at: 0 980 1218
Size: 980 238
SmlI C'TyrA_G
Cuts at: 0 1122 1218
Size: 1122 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 1218
Size: 478 740
TatI w'GTAC_w
Cuts at: 0 371 1218
Size: 371 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 353 385 474 506 1218
Size: 353 32 89 32 712
Fragments arranged by size:
712 353 89 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 736 1218
Size: 736 482
Enzymes that do cut:
AarI AccI AcuI AflII ApoI AvaI BamHI BanI
BanII BbsI BcgI BclI BfrBI Bme1580I BmrI BpmI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrGI BstYI BstZ17I BtgI BtgZI BtsI DraI
EarI Eco57MI EcoNI EcoRV FspI HindIII MmeI MslI
MspA1I NsiI NspI PasI PfoI SapI SexAI SfcI
SmlI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoO109I EcoRI FalI FseI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI