Restriction map for
pFN21AE1877
(Linear) (Six-base) MAPSORT of: KIEE1877.seq Check: 9278 from: 1 to: 1194
KIEE1877.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 511 820 994 1194
Size: 90 421 309 174 200
Fragments arranged by size:
421 309 200 174 90
AccI GT'mk_AC
Cuts at: 0 1175 1194
Size: 1175 19
Acc65I G'GTAC_C
Cuts at: 0 919 1194
Size: 919 275
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1194
Size: 1057 137
AflIII A'CryG_T
Cuts at: 0 872 1194
Size: 872 322
AhdI GACnn_n'nnGTC
Cuts at: 0 609 1194
Size: 609 585
ApaI G_GGCC'C
Cuts at: 0 191 463 1135 1194
Size: 191 272 672 59
Fragments arranged by size:
672 272 191 59
AseI AT'TA_AT
Cuts at: 0 229 1194
Size: 229 965
BanI G'GyrC_C
Cuts at: 0 346 412 919 1194
Size: 346 66 507 275
Fragments arranged by size:
507 346 275 66
BanII G_rGCy'C
Cuts at: 0 191 382 463 569 1069 1135 1194
Size: 191 191 81 106 500 66 59
Fragments arranged by size:
500 191 191 106 81 66 59
BbvCI CC'TCA_GC
Cuts at: 0 482 1194
Size: 482 712
Bme1580I G_kGCm'C
Cuts at: 0 191 463 1135 1194
Size: 191 272 672 59
Fragments arranged by size:
672 272 191 59
BmgBI CAC'GTC
Cuts at: 0 693 1194
Size: 693 501
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 1194
Size: 1083 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 425 1194
Size: 158 267 769
Fragments arranged by size:
769 267 158
Bpu10I CC'TnA_GC
Cuts at: 0 482 1194
Size: 482 712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1194
Size: 653 541
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 1047 1194
Size: 733 314 147
Fragments arranged by size:
733 314 147
BsaAI yAC'GTr
Cuts at: 0 390 1194
Size: 390 804
BsaWI w'CCGG_w
Cuts at: 0 368 1194
Size: 368 826
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 593 623 1194
Size: 385 30 178 30 571
Fragments arranged by size:
571 385 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 221 1194
Size: 221 973
BseYI C'CCAG_C
Cuts at: 0 60 333 1086 1194
Size: 60 273 753 108
Fragments arranged by size:
753 273 108 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 144 1194
Size: 139 5 1050
Fragments arranged by size:
1050 139 5
BsiHKAI G_wGCw'C
Cuts at: 0 569 1194
Size: 569 625
Bsp1286I G_dGCh'C
Cuts at: 0 191 382 463 569 1069 1135 1194
Size: 191 191 81 106 500 66 59
Fragments arranged by size:
500 191 191 106 81 66 59
BspEI T'CCGG_A
Cuts at: 0 368 1194
Size: 368 826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 511 820 994 1194
Size: 90 421 309 174 200
Fragments arranged by size:
421 309 200 174 90
BsrFI r'CCGG_y
Cuts at: 0 343 1194
Size: 343 851
BstXI CCAn_nnnn'nTGG
Cuts at: 0 17 1194
Size: 17 1177
BtgI C'CryG_G
Cuts at: 0 259 449 513 1194
Size: 259 190 64 681
Fragments arranged by size:
681 259 190 64
BtsI GCAGTG_nn'
Cuts at: 0 156 1194
Size: 156 1038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 743 778 1194
Size: 743 35 416
Fragments arranged by size:
743 416 35
DraIII CAC_nnn'GTG
Cuts at: 0 492 1194
Size: 492 702
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1194
Size: 1039 155
EaeI y'GGCC_r
Cuts at: 0 146 1194
Size: 146 1048
EarI CTCTTCn'nnn_
Cuts at: 0 86 719 1194
Size: 86 633 475
Fragments arranged by size:
633 475 86
EcoICRI GAG'CTC
Cuts at: 0 567 1194
Size: 567 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 425 1057 1194
Size: 158 267 632 137
Fragments arranged by size:
632 267 158 137
EcoO109I rG'GnC_Cy
Cuts at: 0 187 460 708 1132 1194
Size: 187 273 248 424 62
Fragments arranged by size:
424 273 248 187 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 595 627 649 681 1194
Size: 385 32 178 32 22 32 513
Fragments arranged by size:
513 385 178 32 32 32 22
KpnI G_GTAC'C
Cuts at: 0 923 1194
Size: 923 271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1194
Size: 839 355
MslI CAynn'nnrTG
Cuts at: 0 968 1194
Size: 968 226
MspA1I CmG'CkG
Cuts at: 0 136 849 1015 1194
Size: 136 713 166 179
Fragments arranged by size:
713 179 166 136
NaeI GCC'GGC
Cuts at: 0 345 1194
Size: 345 849
NgoMIV G'CCGG_C
Cuts at: 0 343 1194
Size: 343 851
PflMI CCAn_nnn'nTGG
Cuts at: 0 197 1194
Size: 197 997
PfoI T'CCnGG_A
Cuts at: 0 877 1194
Size: 877 317
PmlI CAC'GTG
Cuts at: 0 390 1194
Size: 390 804
PpuMI rG'GwC_Cy
Cuts at: 0 708 1194
Size: 708 486
PspOMI G'GGCC_C
Cuts at: 0 187 459 1131 1194
Size: 187 272 672 63
Fragments arranged by size:
672 272 187 63
PstI C_TGCA'G
Cuts at: 0 101 1194
Size: 101 1093
PvuII CAG'CTG
Cuts at: 0 136 849 1194
Size: 136 713 345
Fragments arranged by size:
713 345 136
SacI G_AGCT'C
Cuts at: 0 569 1194
Size: 569 625
SanDI GG'GwC_CC
Cuts at: 0 708 1194
Size: 708 486
SapI GCTCTTCn'nnn_
Cuts at: 0 86 1194
Size: 86 1108
SexAI A'CCwGG_T
Cuts at: 0 797 853 1194
Size: 797 56 341
Fragments arranged by size:
797 341 56
SfcI C'TryA_G
Cuts at: 0 97 615 844 1194
Size: 97 518 229 350
Fragments arranged by size:
518 350 229 97
SmlI C'TyrA_G
Cuts at: 0 668 1194
Size: 668 526
StyI C'CwwG_G
Cuts at: 0 635 761 1136 1194
Size: 635 126 375 58
Fragments arranged by size:
635 375 126 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 771 1194
Size: 540 231 423
Fragments arranged by size:
540 423 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 593 625 1194
Size: 593 32 569
Fragments arranged by size:
593 569 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1143 1194
Size: 1143 51
XmnI GAAnn'nnTTC
Cuts at: 0 547 1194
Size: 547 647
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI ApaI AseI
BanI BanII BbvCI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrFI BstXI BtgI BtsI
CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
Hin4I KpnI MmeI MslI MspA1I NaeI NgoMIV PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SexAI SfcI SmlI StyI TaqII TstI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AlfI AloI
AlwNI ApaLI ApoI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
DraI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI ZraI