Restriction map for
pFN21AE1874
(Linear) (Six-base) MAPSORT of: KIEE1874.seq Check: 8232 from: 1 to: 1188
KIEE1874.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 521 1188
Size: 521 667
AfeI AGC'GCT
Cuts at: 0 217 1188
Size: 217 971
AflIII A'CryG_T
Cuts at: 0 347 548 1188
Size: 347 201 640
Fragments arranged by size:
640 347 201
AgeI A'CCGG_T
Cuts at: 0 861 1188
Size: 861 327
AleI CACnn'nnGTG
Cuts at: 0 712 1188
Size: 712 476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 1188
Size: 811 377
AscI GG'CGCG_CC
Cuts at: 0 487 1188
Size: 487 701
AvaI C'yCGr_G
Cuts at: 0 827 1188
Size: 827 361
BanI G'GyrC_C
Cuts at: 0 95 172 702 809 837 903 1188
Size: 95 77 530 107 28 66 285
Fragments arranged by size:
530 285 107 95 77 66 28
BanII G_rGCy'C
Cuts at: 0 211 1188
Size: 211 977
BbeI G_GCGC'C
Cuts at: 0 99 176 841 907 1188
Size: 99 77 665 66 281
Fragments arranged by size:
665 281 99 77 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 99 133 415 449 1119 1153 1188
Size: 99 34 282 34 670 34 35
Fragments arranged by size:
670 282 99 35 34 34 34
BfrBI ATG'CAT
Cuts at: 0 1140 1188
Size: 1140 48
BglI GCCn_nnn'nGGC
Cuts at: 0 946 1188
Size: 946 242
BlpI GC'TnA_GC
Cuts at: 0 1167 1188
Size: 1167 21
Bme1580I G_kGCm'C
Cuts at: 0 149 236 707 1188
Size: 149 87 471 481
Fragments arranged by size:
481 471 149 87
BmgBI CAC'GTC
Cuts at: 0 543 1188
Size: 543 645
BmrI ACTGGGnnnn_n'
Cuts at: 0 525 678 1188
Size: 525 153 510
Fragments arranged by size:
525 510 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1188
Size: 1060 128
Bpu10I CC'TnA_GC
Cuts at: 0 407 1188
Size: 407 781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1098 1188
Size: 1098 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 763 1188
Size: 763 425
BsaAI yAC'GTr
Cuts at: 0 549 1188
Size: 549 639
BsaHI Gr'CG_yC
Cuts at: 0 96 173 838 904 1188
Size: 96 77 665 66 284
Fragments arranged by size:
665 284 96 77 66
BsaWI w'CCGG_w
Cuts at: 0 861 1188
Size: 861 327
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 656 686 760 790 1188
Size: 656 30 74 30 398
Fragments arranged by size:
656 398 74 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 1147 1188
Size: 283 864 41
Fragments arranged by size:
864 283 41
BseYI C'CCAG_C
Cuts at: 0 383 886 1188
Size: 383 503 302
Fragments arranged by size:
503 383 302
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1072 1188
Size: 640 432 116
Fragments arranged by size:
640 432 116
BsiEI CG_ry'CG
Cuts at: 0 288 465 683 1188
Size: 288 177 218 505
Fragments arranged by size:
505 288 218 177
Bsp1286I G_dGCh'C
Cuts at: 0 149 211 236 707 1188
Size: 149 62 25 471 481
Fragments arranged by size:
481 471 149 62 25
BsrBI CCG'CTC
Cuts at: 0 162 315 629 636 826 981 1188
Size: 162 153 314 7 190 155 207
Fragments arranged by size:
314 207 190 162 155 153 7
BsrDI GCAATG_nn'
Cuts at: 0 332 1188
Size: 332 856
BsrFI r'CCGG_y
Cuts at: 0 84 92 505 711 834 861 875 1188
Size: 84 8 413 206 123 27 14 313
Fragments arranged by size:
413 313 206 123 84 27 14 8
BssHII G'CGCG_C
Cuts at: 0 106 487 871 931 1188
Size: 106 381 384 60 257
Fragments arranged by size:
384 381 257 106 60
BssSI C'ACGA_G
Cuts at: 0 118 229 1188
Size: 118 111 959
Fragments arranged by size:
959 118 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 120 144 1188
Size: 120 24 1044
Fragments arranged by size:
1044 120 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1132 1188
Size: 1132 56
BtgI C'CryG_G
Cuts at: 0 660 962 1188
Size: 660 302 226
Fragments arranged by size:
660 302 226
BtsI GCAGTG_nn'
Cuts at: 0 503 1188
Size: 503 685
DraIII CAC_nnn'GTG
Cuts at: 0 816 1188
Size: 816 372
EaeI y'GGCC_r
Cuts at: 0 90 329 396 680 925 1188
Size: 90 239 67 284 245 263
Fragments arranged by size:
284 263 245 239 90 67
EagI C'GGCC_G
Cuts at: 0 680 1188
Size: 680 508
EarI CTCTTCn'nnn_
Cuts at: 0 727 1188
Size: 727 461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1188
Size: 1060 128
EcoO109I rG'GnC_Cy
Cuts at: 0 225 1188
Size: 225 963
HaeII r_GCGC'y
Cuts at: 0 52 99 176 219 747 841 907 1105
Size: 52 47 77 43 528 94 66 198
Cuts at: 1105 1188
Size: 83
Fragments arranged by size:
528 198 94 83 77 66 52 47
43
KasI G'GCGC_C
Cuts at: 0 95 172 837 903 1188
Size: 95 77 665 66 285
Fragments arranged by size:
665 285 95 77 66
MscI TGG'CCA
Cuts at: 0 331 1188
Size: 331 857
MslI CAynn'nnrTG
Cuts at: 0 337 712 1188
Size: 337 375 476
Fragments arranged by size:
476 375 337
MspA1I CmG'CkG
Cuts at: 0 187 662 1188
Size: 187 475 526
Fragments arranged by size:
526 475 187
NaeI GCC'GGC
Cuts at: 0 94 507 836 877 1188
Size: 94 413 329 41 311
Fragments arranged by size:
413 329 311 94 41
NarI GG'CG_CC
Cuts at: 0 96 173 838 904 1188
Size: 96 77 665 66 284
Fragments arranged by size:
665 284 96 77 66
NgoMIV G'CCGG_C
Cuts at: 0 92 505 834 875 1188
Size: 92 413 329 41 313
Fragments arranged by size:
413 329 313 92 41
NsiI A_TGCA'T
Cuts at: 0 1142 1188
Size: 1142 46
NspI r_CATG'y
Cuts at: 0 351 1140 1188
Size: 351 789 48
Fragments arranged by size:
789 351 48
PasI CC'CwG_GG
Cuts at: 0 168 1188
Size: 168 1020
PciI A'CATG_T
Cuts at: 0 347 1188
Size: 347 841
PfoI T'CCnGG_A
Cuts at: 0 221 600 725 1188
Size: 221 379 125 463
Fragments arranged by size:
463 379 221 125
PmlI CAC'GTG
Cuts at: 0 549 1188
Size: 549 639
PpuMI rG'GwC_Cy
Cuts at: 0 225 1188
Size: 225 963
PspXI vC'TCGA_Gb
Cuts at: 0 827 1188
Size: 827 361
RsrII CG'GwC_CG
Cuts at: 0 579 1188
Size: 579 609
SacII CC_GC'GG
Cuts at: 0 663 1188
Size: 663 525
SapI GCTCTTCn'nnn_
Cuts at: 0 727 1188
Size: 727 461
SexAI A'CCwGG_T
Cuts at: 0 812 1188
Size: 812 376
SfoI GGC'GCC
Cuts at: 0 97 174 839 905 1188
Size: 97 77 665 66 283
Fragments arranged by size:
665 283 97 77 66
SgrAI Cr'CCGG_yG
Cuts at: 0 711 861 1188
Size: 711 150 327
Fragments arranged by size:
711 327 150
SmlI C'TyrA_G
Cuts at: 0 827 1077 1188
Size: 827 250 111
Fragments arranged by size:
827 250 111
SphI G_CATG'C
Cuts at: 0 1140 1188
Size: 1140 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 543 787 1188
Size: 313 230 244 401
Fragments arranged by size:
401 313 244 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 563 595 760 792 1188
Size: 563 32 165 32 396
Fragments arranged by size:
563 396 165 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 517 1188
Size: 517 671
XhoI C'TCGA_G
Cuts at: 0 827 1188
Size: 827 361
Enzymes that do cut:
AccI AfeI AflIII AgeI AleI AlfI AscI AvaI
BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BtgI BtsI
DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII KasI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PfoI PmlI PpuMI PspXI RsrII SacII
SapI SexAI SfoI SgrAI SmlI SphI TaqII TstI
Tth111I XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BglII BmtI BplI
BsaBI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrGI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI PacI PflMI PmeI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfiI SgfI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI XbaI XcmI XmaI
XmnI ZraI