Restriction map for
pFN21AE1873
(Linear) (Six-base) MAPSORT of: KIEE1873.seq Check: 7338 from: 1 to: 1182
KIEE1873.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 1121 1182
Size: 1121 61
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1182
Size: 289 893
AflIII A'CryG_T
Cuts at: 0 323 1182
Size: 323 859
AleI CACnn'nnGTG
Cuts at: 0 73 1182
Size: 73 1109
AlwNI CAG_nnn'CTG
Cuts at: 0 982 1182
Size: 982 200
ApoI r'AATT_y
Cuts at: 0 940 1182
Size: 940 242
AvaI C'yCGr_G
Cuts at: 0 213 1182
Size: 213 969
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1142 1175 1182
Size: 1142 33 7
Fragments arranged by size:
1142 33 7
BamHI G'GATC_C
Cuts at: 0 251 368 789 1182
Size: 251 117 421 393
Fragments arranged by size:
421 393 251 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 686 856 1008 1182
Size: 686 170 152 174
Fragments arranged by size:
686 174 170 152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 1182
Size: 220 34 928
Fragments arranged by size:
928 220 34
BlpI GC'TnA_GC
Cuts at: 0 119 1182
Size: 119 1063
BmgBI CAC'GTC
Cuts at: 0 756 1182
Size: 756 426
BmrI ACTGGGnnnn_n'
Cuts at: 0 374 1182
Size: 374 808
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 1182
Size: 228 954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 650 1182
Size: 411 239 532
Fragments arranged by size:
532 411 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 1097 1182
Size: 1097 85
BsaAI yAC'GTr
Cuts at: 0 324 666 1182
Size: 324 342 516
Fragments arranged by size:
516 342 324
BsaWI w'CCGG_w
Cuts at: 0 296 1043 1182
Size: 296 747 139
Fragments arranged by size:
747 296 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 286 316 1182
Size: 217 30 39 30 866
Fragments arranged by size:
866 217 39 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 6 51 217 1182
Size: 6 45 166 965
Fragments arranged by size:
965 166 45 6
BseYI C'CCAG_C
Cuts at: 0 92 101 157 1182
Size: 92 9 56 1025
Fragments arranged by size:
1025 92 56 9
BsiEI CG_ry'CG
Cuts at: 0 62 1182
Size: 62 1120
BsiHKAI G_wGCw'C
Cuts at: 0 70 1182
Size: 70 1112
BsmI GAATG_Cn'
Cuts at: 0 427 877 1182
Size: 427 450 305
Fragments arranged by size:
450 427 305
Bsp1286I G_dGCh'C
Cuts at: 0 70 1182
Size: 70 1112
BspEI T'CCGG_A
Cuts at: 0 1043 1182
Size: 1043 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 751 1182
Size: 751 431
BsrDI GCAATG_nn'
Cuts at: 0 136 1182
Size: 136 1046
BssSI C'ACGA_G
Cuts at: 0 256 1182
Size: 256 926
BstEII G'GTnAC_C
Cuts at: 0 804 921 1182
Size: 804 117 261
Fragments arranged by size:
804 261 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1182
Size: 471 711
BstYI r'GATC_y
Cuts at: 0 251 368 789 1182
Size: 251 117 421 393
Fragments arranged by size:
421 393 251 117
Bsu36I CC'TnA_GG
Cuts at: 0 34 1182
Size: 34 1148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 782 817 1182
Size: 782 35 365
Fragments arranged by size:
782 365 35
EaeI y'GGCC_r
Cuts at: 0 59 758 1182
Size: 59 699 424
Fragments arranged by size:
699 424 59
EagI C'GGCC_G
Cuts at: 0 59 1182
Size: 59 1123
EarI CTCTTCn'nnn_
Cuts at: 0 503 851 1182
Size: 503 348 331
Fragments arranged by size:
503 348 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 1182
Size: 64 1118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 411 650 1182
Size: 289 122 239 532
Fragments arranged by size:
532 289 239 122
MscI TGG'CCA
Cuts at: 0 760 1182
Size: 760 422
MslI CAynn'nnrTG
Cuts at: 0 73 1075 1182
Size: 73 1002 107
Fragments arranged by size:
1002 107 73
MspA1I CmG'CkG
Cuts at: 0 23 161 1143 1182
Size: 23 138 982 39
Fragments arranged by size:
982 138 39 23
PflMI CCAn_nnn'nTGG
Cuts at: 0 918 1182
Size: 918 264
PfoI T'CCnGG_A
Cuts at: 0 749 1182
Size: 749 433
PmlI CAC'GTG
Cuts at: 0 324 1182
Size: 324 858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 1182
Size: 850 32 300
Fragments arranged by size:
850 300 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 292 324 1182
Size: 292 32 858
Fragments arranged by size:
858 292 32
PstI C_TGCA'G
Cuts at: 0 890 1182
Size: 890 292
PvuII CAG'CTG
Cuts at: 0 161 1143 1182
Size: 161 982 39
Fragments arranged by size:
982 161 39
SapI GCTCTTCn'nnn_
Cuts at: 0 851 1182
Size: 851 331
SfcI C'TryA_G
Cuts at: 0 886 1182
Size: 886 296
SmlI C'TyrA_G
Cuts at: 0 213 1182
Size: 213 969
SspI AAT'ATT
Cuts at: 0 936 1182
Size: 936 246
StyI C'CwwG_G
Cuts at: 0 389 1182
Size: 389 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1182
Size: 1012 170
TatI w'GTAC_w
Cuts at: 0 689 1182
Size: 689 493
Tth111I GACn'n_nGTC
Cuts at: 0 907 1182
Size: 907 275
XhoI C'TCGA_G
Cuts at: 0 213 1182
Size: 213 969
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI AvaI BaeI
BamHI BbsI BcgI BlpI BmgBI BmrI BplI BpmI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrDI BssSI BstEII BstXI
BstYI Bsu36I CspCI EaeI EagI EarI EciI Eco57MI
MscI MslI MspA1I PflMI PfoI PmlI PpiI PsrI
PstI PvuII SapI SfcI SmlI SspI StyI TaqII
TatI Tth111I XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII Bme1580I BmtI Bpu10I BpuEI BsaBI BsaHI BsgI
BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpuMI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TstI XbaI XcmI XmaI XmnI
ZraI