Restriction map for
pFN21AE1872
(Linear) (Six-base) MAPSORT of: KIEE1872.seq Check: 9018 from: 1 to: 1179
KIEE1872.orf
With 182 enzymes: SgfI *
March 22, 2012 15:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 756 1179
Size: 756 423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1179
Size: 951 228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1136 1179
Size: 1136 43
AlwNI CAG_nnn'CTG
Cuts at: 0 342 748 771 810 1014 1179
Size: 342 406 23 39 204 165
Fragments arranged by size:
406 342 204 165 39 23
ApaI G_GGCC'C
Cuts at: 0 1021 1179
Size: 1021 158
ApaLI G'TGCA_C
Cuts at: 0 257 1179
Size: 257 922
ApoI r'AATT_y
Cuts at: 0 277 1047 1179
Size: 277 770 132
Fragments arranged by size:
770 277 132
AvaI C'yCGr_G
Cuts at: 0 854 1179
Size: 854 325
BanII G_rGCy'C
Cuts at: 0 323 740 800 965 1021 1179
Size: 323 417 60 165 56 158
Fragments arranged by size:
417 323 165 158 60 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 1179
Size: 247 932
BlpI GC'TnA_GC
Cuts at: 0 317 734 814 1179
Size: 317 417 80 365
Fragments arranged by size:
417 365 317 80
Bme1580I G_kGCm'C
Cuts at: 0 18 261 1021 1179
Size: 18 243 760 158
Fragments arranged by size:
760 243 158 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 1179
Size: 51 1128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 623 643 814 1056 1130 1156 1179
Size: 460 163 20 171 242 74 26 23
Fragments arranged by size:
460 242 171 163 74 26 23 20
Bpu10I CC'TnA_GC
Cuts at: 0 563 1179
Size: 563 616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1179
Size: 844 335
BsaWI w'CCGG_w
Cuts at: 0 35 113 1179
Size: 35 78 1066
Fragments arranged by size:
1066 78 35
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 514 1179
Size: 511 3 665
Fragments arranged by size:
665 511 3
BseYI C'CCAG_C
Cuts at: 0 86 943 1179
Size: 86 857 236
Fragments arranged by size:
857 236 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 438 889 1179
Size: 6 432 451 290
Fragments arranged by size:
451 432 290 6
BsiHKAI G_wGCw'C
Cuts at: 0 261 476 965 1179
Size: 261 215 489 214
Fragments arranged by size:
489 261 215 214
BsmI GAATG_Cn'
Cuts at: 0 906 1179
Size: 906 273
BsmBI CGTCTCn'nnnn_
Cuts at: 0 363 1179
Size: 363 816
Bsp1286I G_dGCh'C
Cuts at: 0 18 261 323 476 740 800 965 1021
Size: 18 243 62 153 264 60 165 56
Cuts at: 1021 1179
Size: 158
Fragments arranged by size:
264 243 165 158 153 62 60 56
18
BspEI T'CCGG_A
Cuts at: 0 35 113 1179
Size: 35 78 1066
Fragments arranged by size:
1066 78 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 756 1179
Size: 756 423
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 939 953 1179
Size: 810 129 14 226
Fragments arranged by size:
810 226 129 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 236 1179
Size: 236 943
Bsu36I CC'TnA_GG
Cuts at: 0 741 1179
Size: 741 438
BtgI C'CryG_G
Cuts at: 0 1065 1179
Size: 1065 114
BtsI GCAGTG_nn'
Cuts at: 0 655 1179
Size: 655 524
EaeI y'GGCC_r
Cuts at: 0 363 842 1179
Size: 363 479 337
Fragments arranged by size:
479 363 337
EarI CTCTTCn'nnn_
Cuts at: 0 428 868 970 1052 1179
Size: 428 440 102 82 127
Fragments arranged by size:
440 428 127 102 82
EcoICRI GAG'CTC
Cuts at: 0 963 1179
Size: 963 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 623 643 814 951 1056 1130 1156
Size: 460 163 20 171 137 105 74 26
Cuts at: 1156 1179
Size: 23
Fragments arranged by size:
460 171 163 137 105 74 26 23
20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 420 923 1179
Size: 420 503 256
Fragments arranged by size:
503 420 256
EcoO109I rG'GnC_Cy
Cuts at: 0 594 617 1011 1018 1179
Size: 594 23 394 7 161
Fragments arranged by size:
594 394 161 23 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 1179
Size: 162 1017
HaeII r_GCGC'y
Cuts at: 0 810 876 1179
Size: 810 66 303
Fragments arranged by size:
810 303 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 980 1012 1179
Size: 601 32 347 32 167
Fragments arranged by size:
601 347 167 32 32
MscI TGG'CCA
Cuts at: 0 365 844 1179
Size: 365 479 335
Fragments arranged by size:
479 365 335
MslI CAynn'nnrTG
Cuts at: 0 838 1179
Size: 838 341
MspA1I CmG'CkG
Cuts at: 0 232 1179
Size: 232 947
NcoI C'CATG_G
Cuts at: 0 1065 1179
Size: 1065 114
NspI r_CATG'y
Cuts at: 0 133 1179
Size: 133 1046
PflMI CCAn_nnn'nTGG
Cuts at: 0 896 1179
Size: 896 283
PfoI T'CCnGG_A
Cuts at: 0 75 149 476 895 1179
Size: 75 74 327 419 284
Fragments arranged by size:
419 327 284 75 74
PpuMI rG'GwC_Cy
Cuts at: 0 617 1179
Size: 617 562
PspOMI G'GGCC_C
Cuts at: 0 1017 1179
Size: 1017 162
PstI C_TGCA'G
Cuts at: 0 988 1179
Size: 988 191
PvuII CAG'CTG
Cuts at: 0 232 1179
Size: 232 947
SacI G_AGCT'C
Cuts at: 0 965 1179
Size: 965 214
SapI GCTCTTCn'nnn_
Cuts at: 0 970 1179
Size: 970 209
SfcI C'TryA_G
Cuts at: 0 984 1179
Size: 984 195
SmlI C'TyrA_G
Cuts at: 0 823 1179
Size: 823 356
SspI AAT'ATT
Cuts at: 0 191 1179
Size: 191 988
StuI AGG'CCT
Cuts at: 0 181 860 1117 1179
Size: 181 679 257 62
Fragments arranged by size:
679 257 181 62
StyI C'CwwG_G
Cuts at: 0 1065 1179
Size: 1065 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 1179
Size: 33 1146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 210 242 447 479 1016 1048 1179
Size: 210 32 205 32 537 32 131
Fragments arranged by size:
537 210 205 131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 994 1179
Size: 994 185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 532 916 1170 1179
Size: 532 384 254 9
Fragments arranged by size:
532 384 254 9
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanII BbsI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BstAPI BstXI Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MscI MslI MspA1I NcoI NspI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SapI SfcI SmlI SspI
StuI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI BsaI BsaAI BsaBI
BsaHI BsaXI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI XbaI XhoI XmaI XmnI ZraI