(Linear) (Six-base) MAPSORT of: KIEE1870.seq  Check: 4947  from: 1  to: 1173



KIEE1870.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:54  ..

AccI GT'mk_AC

Cuts at:      0      72     125    1173 
   Size:         72      53    1048

  Fragments arranged by size: 

                1048      72      53
 
AclI AA'CG_TT

Cuts at:      0     626    1173 
   Size:        626     547
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490    1173 
   Size:        490     683
 
AleI CACnn'nnGTG

Cuts at:      0     784    1173 
   Size:        784     389
 
AlwNI CAG_nnn'CTG

Cuts at:      0     916    1173 
   Size:        916     257
 
ApoI r'AATT_y

Cuts at:      0     471     611     710    1173 
   Size:        471     140      99     463

  Fragments arranged by size: 

                 471     463     140      99
 
BanII G_rGCy'C

Cuts at:      0       8     229    1173 
   Size:          8     221     944

  Fragments arranged by size: 

                 944     221       8
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     165     199    1173 
   Size:        165      34     974

  Fragments arranged by size: 

                 974     165      34
 
BmgBI CAC'GTC

Cuts at:      0     786    1173 
   Size:        786     387
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1049    1173 
   Size:         40    1009     124

  Fragments arranged by size: 

                1009     124      40
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     383    1173 
   Size:        383     790
 
BsaBI GATnn'nnATC

Cuts at:      0     737    1173 
   Size:        737     436
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     961    1173 
   Size:         62     899     212

  Fragments arranged by size: 

                 899     212      62
 
BsmI GAATG_Cn'

Cuts at:      0     464    1173 
   Size:        464     709
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     229    1173 
   Size:          8     221     944

  Fragments arranged by size: 

                 944     221       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      84     933    1173 
   Size:         84     849     240

  Fragments arranged by size: 

                 849     240      84
 
BsrDI GCAATG_nn'

Cuts at:      0     599     814    1173 
   Size:        599     215     359

  Fragments arranged by size: 

                 599     359     215
 
BsrFI r'CCGG_y

Cuts at:      0     257    1173 
   Size:        257     916
 
BsrGI T'GTAC_A

Cuts at:      0     573    1173 
   Size:        573     600
 
BstZ17I GTA'TAC

Cuts at:      0      73     126    1173 
   Size:         73      53    1047

  Fragments arranged by size: 

                1047      73      53
 
EaeI y'GGCC_r

Cuts at:      0     255    1173 
   Size:        255     918
 
EarI CTCTTCn'nnn_

Cuts at:      0     182     836    1173 
   Size:        182     654     337

  Fragments arranged by size: 

                 654     337     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     490    1049    1173 
   Size:         40     450     559     124

  Fragments arranged by size: 

                 559     450     124      40
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     784    1173 
   Size:        784     389
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     187     219    1173 
   Size:        187      32     954

  Fragments arranged by size: 

                 954     187      32
 
HincII GTy'rAC

Cuts at:      0     105    1173 
   Size:        105    1068
 
HindIII A'AGCT_T

Cuts at:      0     502     691    1173 
   Size:        502     189     482

  Fragments arranged by size: 

                 502     482     189
 
MfeI C'AATT_G

Cuts at:      0     167    1173 
   Size:        167    1006
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    1173 
   Size:        475     698
 
MslI CAynn'nnrTG

Cuts at:      0     784    1173 
   Size:        784     389
 
MspA1I CmG'CkG

Cuts at:      0      32    1173 
   Size:         32    1141
 
NaeI GCC'GGC

Cuts at:      0     259    1173 
   Size:        259     914
 
NgoMIV G'CCGG_C

Cuts at:      0     257    1173 
   Size:        257     916
 
NspI r_CATG'y

Cuts at:      0      48    1141    1173 
   Size:         48    1093      32

  Fragments arranged by size: 

                1093      48      32
 
PstI C_TGCA'G

Cuts at:      0     705     933    1173 
   Size:        705     228     240

  Fragments arranged by size: 

                 705     240     228
 
SapI GCTCTTCn'nnn_

Cuts at:      0     836    1173 
   Size:        836     337
 
SfcI C'TryA_G

Cuts at:      0     701     929    1173 
   Size:        701     228     244

  Fragments arranged by size: 

                 701     244     228
 
SphI G_CATG'C

Cuts at:      0      48    1173 
   Size:         48    1125
 
SspI AAT'ATT

Cuts at:      0     639    1173 
   Size:        639     534
 
StyI C'CwwG_G

Cuts at:      0    1053    1173 
   Size:       1053     120
 
TatI w'GTAC_w

Cuts at:      0     573    1173 
   Size:        573     600
 
Tth111I GACn'n_nGTC

Cuts at:      0     912    1173 
   Size:        912     261
 
XbaI T'CTAG_A

Cuts at:      0     306    1173 
   Size:        306     867
 
XmnI GAAnn'nnTTC

Cuts at:      0     292     710    1173 
   Size:        292     418     463

  Fragments arranged by size: 

                 463     418     292
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AleI    AlwNI     ApoI    BanII     BcgI
    BmgBI     BpmI     BsaI    BsaBI     BsgI     BsmI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI  BstZ17I     EaeI     EarI  Eco57MI     FalI
    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I     NaeI
   NgoMIV     NspI     PstI     SapI     SfcI     SphI     SspI     StyI
     TatI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI     MscI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     StuI     SwaI    TaqII     TstI     XcmI     XhoI     XmaI     ZraI