Restriction map for
pFN21AE1868
(Linear) (Six-base) MAPSORT of: KIEE1868.seq Check: 1421 from: 1 to: 1167
KIEE1868.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 926 1167
Size: 926 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 202 748 1167
Size: 121 81 546 419
Fragments arranged by size:
546 419 121 81
AfeI AGC'GCT
Cuts at: 0 271 1087 1167
Size: 271 816 80
Fragments arranged by size:
816 271 80
AlwNI CAG_nnn'CTG
Cuts at: 0 225 498 1167
Size: 225 273 669
Fragments arranged by size:
669 273 225
AvaI C'yCGr_G
Cuts at: 0 33 503 629 659 1167
Size: 33 470 126 30 508
Fragments arranged by size:
508 470 126 33 30
BanI G'GyrC_C
Cuts at: 0 704 1167
Size: 704 463
BanII G_rGCy'C
Cuts at: 0 14 78 976 1167
Size: 14 64 898 191
Fragments arranged by size:
898 191 64 14
BbeI G_GCGC'C
Cuts at: 0 708 1167
Size: 708 459
BbvCI CC'TCA_GC
Cuts at: 0 469 1167
Size: 469 698
Bme1580I G_kGCm'C
Cuts at: 0 1058 1167
Size: 1058 109
BmgBI CAC'GTC
Cuts at: 0 201 1167
Size: 201 966
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 642 1167
Size: 642 525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 804 896 1167
Size: 25 779 92 271
Fragments arranged by size:
779 271 92 25
Bpu10I CC'TnA_GC
Cuts at: 0 160 469 1167
Size: 160 309 698
Fragments arranged by size:
698 309 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 313 534 1167
Size: 310 3 221 633
Fragments arranged by size:
633 310 221 3
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 953 1167
Size: 52 901 214
Fragments arranged by size:
901 214 52
BsaHI Gr'CG_yC
Cuts at: 0 208 705 1167
Size: 208 497 462
Fragments arranged by size:
497 462 208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 908 938 1167
Size: 908 30 229
Fragments arranged by size:
908 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1167
Size: 22 1145
BseYI C'CCAG_C
Cuts at: 0 376 532 556 834 1167
Size: 376 156 24 278 333
Fragments arranged by size:
376 333 278 156 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 211 1167
Size: 87 124 956
Fragments arranged by size:
956 124 87
BsiEI CG_ry'CG
Cuts at: 0 19 303 1167
Size: 19 284 864
Fragments arranged by size:
864 284 19
BsiHKAI G_wGCw'C
Cuts at: 0 1067 1167
Size: 1067 100
BsmI GAATG_Cn'
Cuts at: 0 683 1167
Size: 683 484
Bsp1286I G_dGCh'C
Cuts at: 0 14 78 976 1058 1067 1167
Size: 14 64 898 82 9 100
Fragments arranged by size:
898 100 82 64 14 9
BsrBI CCG'CTC
Cuts at: 0 831 1167
Size: 831 336
BsrDI GCAATG_nn'
Cuts at: 0 90 1092 1167
Size: 90 1002 75
Fragments arranged by size:
1002 90 75
BssSI C'ACGA_G
Cuts at: 0 241 1076 1167
Size: 241 835 91
Fragments arranged by size:
835 241 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 1040 1062 1167
Size: 258 782 22 105
Fragments arranged by size:
782 258 105 22
BtgI C'CryG_G
Cuts at: 0 19 398 1050 1167
Size: 19 379 652 117
Fragments arranged by size:
652 379 117 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 216 1167
Size: 216 951
BtsI GCAGTG_nn'
Cuts at: 0 456 815 1167
Size: 456 359 352
Fragments arranged by size:
456 359 352
DraIII CAC_nnn'GTG
Cuts at: 0 140 1062 1167
Size: 140 922 105
Fragments arranged by size:
922 140 105
EaeI y'GGCC_r
Cuts at: 0 16 128 300 1047 1167
Size: 16 112 172 747 120
Fragments arranged by size:
747 172 120 112 16
EagI C'GGCC_G
Cuts at: 0 16 300 1167
Size: 16 284 867
Fragments arranged by size:
867 284 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 17 1167
Size: 17 1150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 121 202 748 804 896 1167
Size: 25 96 81 546 56 92 271
Fragments arranged by size:
546 271 96 92 81 56 25
FspI TGC'GCA
Cuts at: 0 190 1167
Size: 190 977
HaeII r_GCGC'y
Cuts at: 0 273 323 708 1089 1167
Size: 273 50 385 381 78
Fragments arranged by size:
385 381 273 78 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 908 940 1167
Size: 908 32 227
Fragments arranged by size:
908 227 32
KasI G'GCGC_C
Cuts at: 0 704 1167
Size: 704 463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1167
Size: 1001 166
MslI CAynn'nnrTG
Cuts at: 0 582 1167
Size: 582 585
MspA1I CmG'CkG
Cuts at: 0 21 123 258 489 764 1167
Size: 21 102 135 231 275 403
Fragments arranged by size:
403 275 231 135 102 21
NarI GG'CG_CC
Cuts at: 0 705 1167
Size: 705 462
NotI GC'GGCC_GC
Cuts at: 0 300 1167
Size: 300 867
NspI r_CATG'y
Cuts at: 0 782 1167
Size: 782 385
PasI CC'CwG_GG
Cuts at: 0 979 1167
Size: 979 188
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 1167
Size: 606 561
PstI C_TGCA'G
Cuts at: 0 409 488 494 1167
Size: 409 79 6 673
Fragments arranged by size:
673 409 79 6
PvuII CAG'CTG
Cuts at: 0 258 489 764 1167
Size: 258 231 275 403
Fragments arranged by size:
403 275 258 231
SacII CC_GC'GG
Cuts at: 0 22 1167
Size: 22 1145
SfcI C'TryA_G
Cuts at: 0 405 484 490 1167
Size: 405 79 6 677
Fragments arranged by size:
677 405 79 6
SfoI GGC'GCC
Cuts at: 0 706 1167
Size: 706 461
SmaI CCC'GGG
Cuts at: 0 661 1167
Size: 661 506
SmlI C'TyrA_G
Cuts at: 0 289 328 549 1167
Size: 289 39 221 618
Fragments arranged by size:
618 289 221 39
StuI AGG'CCT
Cuts at: 0 179 1167
Size: 179 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1140 1167
Size: 1140 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 435 467 1167
Size: 435 32 700
Fragments arranged by size:
700 435 32
Tth111I GACn'n_nGTC
Cuts at: 0 150 207 1167
Size: 150 57 960
Fragments arranged by size:
960 150 57
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1017 1167
Size: 1017 150
XmaI C'CCGG_G
Cuts at: 0 659 1167
Size: 659 508
Enzymes that do cut:
AccI AcuI AfeI AlwNI AvaI BanI BanII BbeI
BbvCI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BsrBI BsrDI BssSI BstAPI BtgI BtgZI BtsI
DraIII EaeI EagI EciI Eco57MI FspI HaeII Hin4I
KasI MmeI MslI MspA1I NarI NotI NspI PasI
PflMI PstI PvuII SacII SfcI SfoI SmaI SmlI
StuI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BsaAI BsaBI
BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EarI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI
SwaI TatI XbaI XhoI XmnI ZraI