Restriction map for
pFN21AE1867
(Linear) (Six-base) MAPSORT of: KIEE1867.seq Check: 6115 from: 1 to: 870
KIEE1867.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 574 870
Size: 574 296
AatII G_ACGT'C
Cuts at: 0 776 870
Size: 776 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 250 870
Size: 237 13 620
Fragments arranged by size:
620 237 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 347 870
Size: 347 523
AlwNI CAG_nnn'CTG
Cuts at: 0 267 285 870
Size: 267 18 585
Fragments arranged by size:
585 267 18
ApaI G_GGCC'C
Cuts at: 0 495 870
Size: 495 375
ApoI r'AATT_y
Cuts at: 0 455 870
Size: 455 415
BamHI G'GATC_C
Cuts at: 0 485 870
Size: 485 385
BanI G'GyrC_C
Cuts at: 0 214 870
Size: 214 656
BanII G_rGCy'C
Cuts at: 0 166 495 590 870
Size: 166 329 95 280
Fragments arranged by size:
329 280 166 95
Bme1580I G_kGCm'C
Cuts at: 0 153 217 495 760 870
Size: 153 64 278 265 110
Fragments arranged by size:
278 265 153 110 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 650 870
Size: 650 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 870
Size: 458 412
Bpu10I CC'TnA_GC
Cuts at: 0 165 870
Size: 165 705
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 870
Size: 34 836
BsaHI Gr'CG_yC
Cuts at: 0 773 870
Size: 773 97
BsaWI w'CCGG_w
Cuts at: 0 314 870
Size: 314 556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 42 72 88 118 870
Size: 42 30 16 30 752
Fragments arranged by size:
752 42 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 109 870
Size: 7 102 761
Fragments arranged by size:
761 102 7
BseYI C'CCAG_C
Cuts at: 0 146 174 197 870
Size: 146 28 23 673
Fragments arranged by size:
673 146 28 23
BsiHKAI G_wGCw'C
Cuts at: 0 515 870
Size: 515 355
BsmI GAATG_Cn'
Cuts at: 0 399 870
Size: 399 471
Bsp1286I G_dGCh'C
Cuts at: 0 153 166 217 495 515 590 760 870
Size: 153 13 51 278 20 75 170 110
Fragments arranged by size:
278 170 153 110 75 51 20 13
BspEI T'CCGG_A
Cuts at: 0 314 870
Size: 314 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 574 870
Size: 191 383 296
Fragments arranged by size:
383 296 191
BstEII G'GTnAC_C
Cuts at: 0 178 870
Size: 178 692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 638 870
Size: 638 232
BstYI r'GATC_y
Cuts at: 0 365 485 870
Size: 365 120 385
Fragments arranged by size:
385 365 120
BtgI C'CryG_G
Cuts at: 0 243 870
Size: 243 627
DraIII CAC_nnn'GTG
Cuts at: 0 157 870
Size: 157 713
EaeI y'GGCC_r
Cuts at: 0 669 870
Size: 669 201
EarI CTCTTCn'nnn_
Cuts at: 0 25 870
Size: 25 845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 250 458 870
Size: 237 13 208 412
Fragments arranged by size:
412 237 208 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 870
Size: 22 848
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 470 502 758 790 795 827
Size: 88 32 350 32 256 32 5 32
Cuts at: 827 870
Size: 43
Fragments arranged by size:
350 256 88 43 32 32 32 32
5
HindIII A'AGCT_T
Cuts at: 0 326 870
Size: 326 544
MspA1I CmG'CkG
Cuts at: 0 102 146 753 870
Size: 102 44 607 117
Fragments arranged by size:
607 117 102 44
NcoI C'CATG_G
Cuts at: 0 243 870
Size: 243 627
NspI r_CATG'y
Cuts at: 0 117 870
Size: 117 753
PfoI T'CCnGG_A
Cuts at: 0 474 870
Size: 474 396
PspOMI G'GGCC_C
Cuts at: 0 491 870
Size: 491 379
PstI C_TGCA'G
Cuts at: 0 188 680 870
Size: 188 492 190
Fragments arranged by size:
492 190 188
PvuII CAG'CTG
Cuts at: 0 102 146 870
Size: 102 44 724
Fragments arranged by size:
724 102 44
SfcI C'TryA_G
Cuts at: 0 184 384 676 870
Size: 184 200 292 194
Fragments arranged by size:
292 200 194 184
SphI G_CATG'C
Cuts at: 0 117 870
Size: 117 753
StuI AGG'CCT
Cuts at: 0 637 870
Size: 637 233
StyI C'CwwG_G
Cuts at: 0 243 450 870
Size: 243 207 420
Fragments arranged by size:
420 243 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 711 870
Size: 166 545 159
Fragments arranged by size:
545 166 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 723 755 870
Size: 723 32 115
Fragments arranged by size:
723 115 32
Tth111I GACn'n_nGTC
Cuts at: 0 205 870
Size: 205 665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 376 716 870
Size: 376 340 154
Fragments arranged by size:
376 340 154
ZraI GAC'GTC
Cuts at: 0 774 870
Size: 774 96
Enzymes that do cut:
AarI AatII AcuI AlfI AlwNI ApaI ApoI BamHI
BanI BanII Bme1580I BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspMI BstEII BstXI BstYI BtgI DraIII EaeI EarI
Eco57MI EcoNI Hin4I HindIII MspA1I NcoI NspI PfoI
PspOMI PstI PvuII SfcI SphI StuI StyI TaqII
TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI