Restriction map for
pFN21AE1854
(Linear) (Six-base) MAPSORT of: KIEE1854.seq Check: 1803 from: 1 to: 1059
KIEE1854.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AatII G_ACGT'C
Cuts at: 0 230 1059
Size: 230 829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1059
Size: 166 893
AflIII A'CryG_T
Cuts at: 0 599 614 1059
Size: 599 15 445
Fragments arranged by size:
599 445 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 285 317 1059
Size: 285 32 742
Fragments arranged by size:
742 285 32
ApaI G_GGCC'C
Cuts at: 0 279 1059
Size: 279 780
ApaLI G'TGCA_C
Cuts at: 0 787 1059
Size: 787 272
AvaI C'yCGr_G
Cuts at: 0 234 485 859 1059
Size: 234 251 374 200
Fragments arranged by size:
374 251 234 200
BanI G'GyrC_C
Cuts at: 0 197 238 501 943 1059
Size: 197 41 263 442 116
Fragments arranged by size:
442 263 197 116 41
BanII G_rGCy'C
Cuts at: 0 279 398 650 1059
Size: 279 119 252 409
Fragments arranged by size:
409 279 252 119
BbeI G_GCGC'C
Cuts at: 0 505 1059
Size: 505 554
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 928 1059
Size: 740 188 131
Fragments arranged by size:
740 188 131
BbvCI CC'TCA_GC
Cuts at: 0 77 1059
Size: 77 982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 270 304 1059
Size: 270 34 755
Fragments arranged by size:
755 270 34
BglI GCCn_nnn'nGGC
Cuts at: 0 799 1059
Size: 799 260
BglII A'GATC_T
Cuts at: 0 935 1059
Size: 935 124
Bme1580I G_kGCm'C
Cuts at: 0 241 279 791 1059
Size: 241 38 512 268
Fragments arranged by size:
512 268 241 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 1059
Size: 394 665
Bpu10I CC'TnA_GC
Cuts at: 0 77 1059
Size: 77 982
BsaAI yAC'GTr
Cuts at: 0 357 786 1059
Size: 357 429 273
Fragments arranged by size:
429 357 273
BsaHI Gr'CG_yC
Cuts at: 0 227 502 1059
Size: 227 275 557
Fragments arranged by size:
557 275 227
BsaWI w'CCGG_w
Cuts at: 0 1049 1059
Size: 1049 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 163 193 586 616 1059
Size: 94 30 39 30 393 30 443
Fragments arranged by size:
443 393 94 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 400 946 1059
Size: 151 249 546 113
Fragments arranged by size:
546 249 151 113
BsiHKAI G_wGCw'C
Cuts at: 0 398 479 791 1059
Size: 398 81 312 268
Fragments arranged by size:
398 312 268 81
Bsp1286I G_dGCh'C
Cuts at: 0 241 279 398 479 650 791 1059
Size: 241 38 119 81 171 141 268
Fragments arranged by size:
268 241 171 141 119 81 38
BsrFI r'CCGG_y
Cuts at: 0 194 241 560 841 1059
Size: 194 47 319 281 218
Fragments arranged by size:
319 281 218 194 47
BssSI C'ACGA_G
Cuts at: 0 133 1059
Size: 133 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 251 1059
Size: 251 808
BstEII G'GTnAC_C
Cuts at: 0 360 681 1059
Size: 360 321 378
Fragments arranged by size:
378 360 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 1002 1059
Size: 678 324 57
Fragments arranged by size:
678 324 57
BstYI r'GATC_y
Cuts at: 0 581 935 1059
Size: 581 354 124
Fragments arranged by size:
581 354 124
BtsI GCAGTG_nn'
Cuts at: 0 66 1059
Size: 66 993
DraIII CAC_nnn'GTG
Cuts at: 0 360 1059
Size: 360 699
EaeI y'GGCC_r
Cuts at: 0 45 548 635 1059
Size: 45 503 87 424
Fragments arranged by size:
503 424 87 45
EarI CTCTTCn'nnn_
Cuts at: 0 208 454 1059
Size: 208 246 605
Fragments arranged by size:
605 246 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 1059
Size: 67 992
EcoICRI GAG'CTC
Cuts at: 0 396 1059
Size: 396 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1059
Size: 166 893
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 700 1059
Size: 205 495 359
Fragments arranged by size:
495 359 205
EcoO109I rG'GnC_Cy
Cuts at: 0 275 981 1059
Size: 275 706 78
Fragments arranged by size:
706 275 78
FspI TGC'GCA
Cuts at: 0 160 1059
Size: 160 899
FspAI rTGC'GCAy
Cuts at: 0 160 1059
Size: 160 899
HaeII r_GCGC'y
Cuts at: 0 505 1059
Size: 505 554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 217 249 1059
Size: 73 32 112 32 810
Fragments arranged by size:
810 112 73 32 32
KasI G'GCGC_C
Cuts at: 0 501 1059
Size: 501 558
MscI TGG'CCA
Cuts at: 0 47 637 1059
Size: 47 590 422
Fragments arranged by size:
590 422 47
MslI CAynn'nnrTG
Cuts at: 0 34 952 1059
Size: 34 918 107
Fragments arranged by size:
918 107 34
MspA1I CmG'CkG
Cuts at: 0 678 1020 1059
Size: 678 342 39
Fragments arranged by size:
678 342 39
NarI GG'CG_CC
Cuts at: 0 502 1059
Size: 502 557
NspI r_CATG'y
Cuts at: 0 258 618 957 1059
Size: 258 360 339 102
Fragments arranged by size:
360 339 258 102
PciI A'CATG_T
Cuts at: 0 614 1059
Size: 614 445
PmlI CAC'GTG
Cuts at: 0 357 1059
Size: 357 702
PpuMI rG'GwC_Cy
Cuts at: 0 981 1059
Size: 981 78
PspOMI G'GGCC_C
Cuts at: 0 275 1059
Size: 275 784
PvuII CAG'CTG
Cuts at: 0 678 1020 1059
Size: 678 342 39
Fragments arranged by size:
678 342 39
SacI G_AGCT'C
Cuts at: 0 398 1059
Size: 398 661
SfoI GGC'GCC
Cuts at: 0 503 1059
Size: 503 556
SmaI CCC'GGG
Cuts at: 0 487 1059
Size: 487 572
SphI G_CATG'C
Cuts at: 0 957 1059
Size: 957 102
StyI C'CwwG_G
Cuts at: 0 266 1059
Size: 266 793
TatI w'GTAC_w
Cuts at: 0 566 1059
Size: 566 493
XmaI C'CCGG_G
Cuts at: 0 485 1059
Size: 485 574
ZraI GAC'GTC
Cuts at: 0 228 1059
Size: 228 831
Enzymes that do cut:
AatII AcuI AflIII AloI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I
BmrI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI
Bsp1286I BsrFI BssSI BstAPI BstEII BstXI BstYI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I KasI MscI MslI MspA1I
NarI NspI PciI PmlI PpuMI PspOMI PvuII SacI
SfoI SmaI SphI StyI TatI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BlpI BmgBI BmtI
BplI BpmI BpuEI BsaI BsaBI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BstBI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI
HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI